In vitro detection of acaricide resistance in Boophilus microplus (Acari: Ixodidae)

2003 ◽  
pp. 265-271
Author(s):  
Mario A. Villarino ◽  
G. Gale Wagner ◽  
John E. George
Keyword(s):  
Boophilus Microplus ◽  
Acaricide Resistance

In vitro Efficacy of Deltamethrin against Hyalomma anatolicum anatolicum

2019 ◽  
Vol 15 (01) ◽  
pp. 55-56
Author(s):  
Pratibha Thakur ◽  
R K Bagherwal ◽  
A K Jayraw ◽  
A K Soni
Keyword(s):  
Tick Control ◽  
Boophilus Microplus ◽  
Acaricide Resistance ◽  
Hyalomma Anatolicum Anatolicum ◽  
Nerve Ganglion ◽  
Cattle Health ◽  
College Farm

Tick and tick-borne diseases such as theileriosis,babesiosis, and Anaplasmosis pose a constant threato cattle health. Rhipicephalus (Boophilus) microplus andHyalomma anatolicum are the most commonly foundcattle tick species and cause significant economic lossesto dairy and leather industries by adversely affecting themilk production and quality of hides. Tick control strategyinvolves mainly the use of synthetic acaricides which resultsin the development of acaricide resistance. Deltamethrinacts on the parasites by contact and has strong lipophilicaction thus penetrates the cuticle nerve ganglion and block the motor activity. The presentstudy was aimed at the determination of in vitro efficacyof Deltamethrin against H. anatolicum ticks collected fromhealthy cattle of college farm and nearby dairy farms.

scholarly journals Acaricide resistance of Rhipicephalus (Boophilus) microplus in State of Mato Grosso do Sul, Brazil

2011 ◽  
Vol 20 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Renato Andreotti ◽  
Felix David Guerrero ◽  
Mariana Aparecida Soares ◽  
Jacqueline Cavalcante Barros ◽  
Robert John Miller ◽  
...  
Keyword(s):  
Gene Mutation ◽  
Pyrethroid Resistance ◽  
Immersion Test ◽  
The State ◽  
Boophilus Microplus ◽  
Acaricide Resistance ◽  
Mato Grosso ◽  
Pcr Assays ◽  
Mato Grosso Do Sul

This study was conducted to obtain an epidemiological view of acaricide resistance in populations of Rhipicephalus (Boophilus) microplus in the State of Mato Grosso do Sul. Twenty-four tick samples were collected from municipalities in the State where farmers had reported concerns about resistance to or failure of tick control. These ticks were subjected to in vitro resistance detection assays using the adult immersion test (AIT). The efficacy of alpha-cypermethrin, cypermethrin and amitraz treatments on samples collected throughout the State was generally poor. AIT showed efficacy > 90% from the use of DDVP + chlorfenvinphos) (20 out of 21 municipalities), dichlorvos + cypermethrin (10 out of 16 municipalities) and cypermethrin + citronella + chlorpyrifos + piperonyl butoxide (20 out of 21 municipalities). PCR assays were used to detect the presence of pyrethroid resistance-associated sodium channel gene mutation. Larvae from three different populations that had previously been diagnosed as pyrethroid-resistant, through AIT, were evaluated. The PCR assays showed that the pyrethroid resistance-associated gene mutation was absent from these three populations. This study confirms that the emergence of resistance is a constant challenge for the livestock industry, and that development of resistance continues to be a major driver for new antiparasitic drugs to be developed.

In vitro Detection of Acaricide Resistance in Boophilus microplus (Acari: Ixodidae)

2002 ◽  
Vol 28 (1-4) ◽  
pp. 265-271 ◽  
Author(s):  
Mario A. Villarino ◽  
G. Gale Wagner ◽  
John E. George
Keyword(s):  
Boophilus Microplus ◽  
Acaricide Resistance

scholarly journals In vitro activity of the essential oil from Hesperozygis myrtoides on Rhipicephalus (Boophilus) microplus and Haemonchus contortus

2017 ◽  
Vol 27 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Caroline V.V. Castilho ◽  
Rafaela R. Fantatto ◽  
Yousmel A. Gaínza ◽  
Humberto R. Bizzo ◽  
Nancy S. Barbi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Haemonchus Contortus ◽  
Boophilus Microplus ◽  
Vitro Activity ◽  
In Vitro Activity

scholarly journals In vitro activity of the hydroalcoholic extract of Chenopodium ambrosioides against engorged females of Rhipicephalus (Boophilus) microplus

2018 ◽  
Vol 84 (0) ◽  
Author(s):  
Eduardo Oliveira ◽  
Manoela da Silva ◽  
Lew Sprenger ◽  
Daniela Pedrassani
Keyword(s):  
High Efficiency ◽  
Boophilus Microplus ◽  
Vitro Activity ◽  
Economic Losses ◽  
Cattle Tick ◽  
Distilled Water ◽  
In Vitro Activity ◽  
Chenopodium Ambrosioides ◽  
Cattle Ticks

ABSTRACT: Rhipicephalus (Boophilus) microplus, known as the cattle tick, is a cause of great economic losses for dairy cattle farming because of its high frequency of occurrence and the difficulty in controlling it. The aim of this study was to evaluate the in vitro activity of Chenopodium ambrosioides extract on R. (B.) microplus. For this purpose, 125 females were selected and classified into five groups according to their weight, in order to ensure that the females used presented homogeneous weight. The treatments comprised 40 and 60% extracts of Chenopodium ambrosioides, distilled water, ethanol (70ºGL) and 12.5% amitraz. The extracts of C. ambrosioides (40 and 60%) showed effectiveness of 99.7 and 100% and higher percentages of dead females than the other treatments: 64 and 96%, respectively (p<0.001). In the groups exposed to distilled water and ethanol (70º GL), 92 and 88% of the females maintained oviposition. In the females exposed to 40 and 60% extracts, oviposition of 36 and 4% occurred, respectively. It was concluded that the extract of Chenopodium ambrosioides, at both concentration evaluated, had high efficiency against engorged females of cattle ticks.

scholarly journals Control of bovine tick [Rhipicephalus (Boophilus) microplus] with Brunfelsia uniflora leaf extract

2019 ◽  
pp. 903-910
Author(s):  
Elaine Yae Yamashita Sugauara ◽  
ElisângelaYumi Sugauara ◽  
Rosangela Rumi Sugauara ◽  
Wanessa de Campos Bortolucci ◽  
Herika Line Marko de Oliveira ◽  
...  
Keyword(s):  
Ethyl Ester ◽  
Leaf Extract ◽  
Acid Ethyl Ester ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Ex Situ ◽  
In Vitro Tests ◽  
Free Living ◽  
Crude Leaf Extract

Bovine tick has caused losses in livestock production profitability in Brazil. However, tick control has caused resistance of these ectoparasites against utilized acaricides. Alternative tick controls have been utilizing plants as sources of effective botanical acaricides. Brunfelsia uniflora is a Brazilian plant with antimicrobial and antioxidant activity; however, there are no reports on its acaricidal activity. Therefore, this study aimed to evaluate the chemical composition of B. uniflora leaf ethanolic extract and its efficiency to control bovine tick in vitro and free-living stage ex situ. The crude leaf extract was analyzed by gas chromatographer coupled to mass spectrometer (GC-MS) with identification of 17 compounds. The major compounds were phytol (22.96%), 9,12,15-octadecatrienoic acid, ethyl ester (Z,Z,Z) (21.18%), hexadecanoic acid, ethyl ester (12.74%) and vitamin E (8.77%). The crude extract presented acaricidal activity in vitro against ingurgitated adult females, larvae and eggs of bovine tick. The LC99.9 for larvae was 103.21 mg mL-1 in in vitro tests and was 100% efficient for ex situ larva test (free-living stage). B. uniflora leaf extract is an alternative for the control of the bovine tick cycle, mainly in the free-living stage (non-parasitic stage) under field conditions.

ANÁLISE IN VITRO DA EFICÁCIA CARRAPATICIDA E DA ATIVIDADE REPELENTE DA ÁGUA DE MANIPUERIA SOBRE Boophilus microplus NO EXTREMO SUL DA BAHIA

2020 ◽  
pp. 126-138
Author(s):  
Breno Meirelles Costa Brito Passos ◽  
Lívia Santos Lima Lemos ◽  
Gisele Lopes de Oliveira ◽  
Jeilly Vivianne Ribeiro da S. B. de Carvalho ◽  
Paulo Sérgio Onofre ◽  
...  
Keyword(s):  
Boophilus Microplus

scholarly journals Eficácia de diferentes formulações de acaricidas sobre larvas de Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) e Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Acari: Ixodidae)

Biotemas ◽  
2017 ◽  
Vol 30 (1) ◽  
pp. 65
Author(s):  
Luciano Melo Souza ◽  
Marco Antonio Andrade Belo ◽  
Ives Charlie Silva
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Boophilus Microplus ◽  
De Se

http://dx.doi.org/10.5007/2175-7925.2017v30n1p65O parasitismo por ácaros sempre foi objeto de preocupações e perdas relacionadas à produção animal, assim como à transmissibilidade de agentes etiológicos de importantes doenças; e aproximadamente 95% das formas evolutivas dos carrapatos em fase não parasitária encontram-se no ambiente. Com base na importância de se controlar estágios larvares dos ixodídeos no ambiente, este estudo avaliou a eficácia de acaricidas in vitro sobre larvas de Rhipicephalus (Boophilus) microplus e Rhipicephalus sanguineus. Foram testadas: Cipermetrina 15% (T1); Deltametrina 25% (T2); associação de Cipermetrina 5% + Diclorvós 45% + Butóxido de piperolina 25% (T3); associação de Cipermetrina 15% + Clorpiriphos 25% + Citronela 1% (T4) e Amitraz 12,5% (T5). Para cada tratamento foram realizadas nove repetições contendo 20 larvas. As observações de motilidade foram realizadas: 1, 5, 10, 20 e 30 minutos após o tratamento (MPT); 1, 2, 6, 12 e 24 h após o tratamento (HPT); 5 dias após o tratamento (DPT). Os testes das larvas de R. (B.) microplus e R. sanguineus com deltametrina 25% serviram de controle positivo e demonstraram perda de motilidade de 100% das larvas com duas HPT para ambas de ixodídeos. O R. (B.) microplus apresentou maior sensibilidade aos desafios contendo a cipermetrina 15% e 5% associada ao diclorvós e butóxido de piperonila. Larvas de R. sanguineus apresentaram maior sensibilidade em menor tempo ao tratamento com amitraz a 12,5%. Contudo, apesar das diferenças no efeito acaricida observadas no decorrer dos tempos, obteve-se eficácia de 100% para todos os compostos inseticidas sobre os instares das duas espécies 12 HPT, demonstrando que, quando utilizados nas doses recomendadas pelos fabricantes, os produtos comerciais podem ser eficazes em estratégias de controle ambiental das larvas.

scholarly journals Avaliação in vitro de uma cepa de campo de Boophilus microplus (Acari : Ixodidae) resistente à amitraz

2003 ◽  
Vol 33 (4) ◽  
pp. 737-742 ◽  
Author(s):  
Minerva Santamaría Vargas ◽  
Noé Soberanes Céspedes ◽  
Hugo Fragoso Sánchez ◽  
João Ricardo Martins ◽  
Carlos Octávio Cordovés Céspedes
Keyword(s):  
Rio Grande ◽  
Boophilus Microplus ◽  
Rio Grande Do Sul

O uso dos acaricidas químicos ainda se constitui no principal instrumento de controle do carrapato bovino Boophilus microplus. No sul do Brasil, o amitraz aplicado por imersão e aspersão é o ingrediente ativo mais utilizado, nos últimos anos, contra as cepas de carrapatos resistentes aos organofosforados (OF) e piretróides sintéticos (PS). Em conseqüência, torna-se importante a realização de investigações que possam contribuir para prolongar a vida útil desse ixodicida. No presente estudo, foi analisado o comportamento toxicológico de uma cepa de B. microplus colhida na localidade de Alegrete, Rio Grande do Sul, usando-se químicos da família dos OF, PS, misturas OF/PS, além do amitraz. Os resultados indicaram que a cepa denominada "Santa Luiza" apresentou fatores de resistência (FR) que variaram entre 2,3 e 3,95 para OF, 23,3 e 147,56 para PS e de 3,76 a 21.57 para amitraz em testes realizados com larvas de carrapatos. A caracterização e purificação de uma cepa de B. microplus resistente ao amitraz permitem seu uso como cepa de referência para a avaliação biológica de ixodicidas alternativos, além de padronizar e validar métodos de diagnósticos toxicológicos que possam detectar resistência frente a esse acaricida.

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