MicroRNA-1 Expression and Function in Hyalomma Anatolicum anatolicum (Acari: Ixodidae) Ticks

MicroRNAs act as mRNA post-transcriptional regulators, playing important roles in cell differentiation, transcriptional regulation, growth, and development. In this study, microRNA expression profiles of Hyalomma anatolicum anatolicum ticks at different developmental stages were detected by high-throughput sequencing and functionally assessed. In total, 2,585,169, 1,252,678, 1,558,217, and 1,155,283 unique reads were obtained from eggs, larvae, nymphs, and adults, respectively, with 42, 46, 45, and 41 conserved microRNAs in these stages, respectively. Using eggs as a control, 48, 43, and 39 microRNAs were upregulated, and 3, 10, and 9 were downregulated in larvae, nymphs, and adults, respectively. MicroRNA-1 (miR-1) was expressed in high abundance throughout Ha. anatolicum development, with an average of nearly one million transcripts, and it is highly conserved among tick species. Quantitative real-time PCR (qPCR) showed that miR-1 expression gradually increased with tick development, reaching the highest level at engorgement. Differential tissue expression was detected, with significantly higher levels in the salivary glands and epidermis than in the midgut. Inhibition assays showed no significant change in body weight or spawning time or amount between experimental and control groups, but there was a significant difference (p < 0.01) in engorgement time. With miR-1 inhibition, ticks displayed obvious deformities during later development. To more fully explain the microRNA mechanism of action, the miR-1 cluster was analyzed according to the target gene; members that jointly act on Hsp60 include miR-5, miR-994, miR-969, and miR-1011. Therefore, microRNAs are critical for normal tick development, and the primary structure of the mature sequence of miR-1 is highly conserved. Nonetheless, different developmental stages and tissues show different expression patterns, with a certain role in prolonging feeding. miR-1, together with other cluster members, regulates mRNA function and may be used as a molecular marker for species origin, evolution analysis, and internal reference gene selection.

MicroRNA-1 promotes the development of and prolongs engorgement time in Hyalomma anatolicum anatolicum (Acari: Ixodidae) ticks

MicroRNAs act as mRNA posttranscriptional regulators, playing important roles in cell differentiation, transcriptional regulation, growth and development. In this study, microRNA expression profiles of Hyalomma anatolicum anatolicum ticks at different developmental stages were detected by high-throughput sequencing and functionally assessed. In total, 2,585,169, 1,252,678, 1,558,217 and 1,155,283 unique reads were obtained from eggs, larvae, nymphs and adults, respectively, with 42, 46, 45 and 41 conserved microRNAs in these stages, respectively. Using eggs as a control, 48, 43 and 39 microRNAs were upregulated and 3, 10 and 9 downregulated in larvae, nymphs and adults, respectively. microRNA-1 (miR-1) was expressed in high abundance throughout Ha. anatolicum development, with an average of nearly one million transcripts, and it is highly conserved among tick species. Quantitative real-time PCR (qPCR) showed that miR-1 expression gradually increased with tick development, reaching the highest level at engorgement. Differential tissue expression was detected, with significantly higher levels in the salivary glands and epidermis than in the midgut. Inhibition assays showed no significant change in body weight or spawning time or amount between experimental and control groups, but there was a significant difference (p<0.01) in engorgement time. With miR-1 inhibition, ticks displayed obvious deformities during later development. To more fully explain the microRNA mechanism of action, the miR-1 family was analyzed regarding target gene; members acting on Hsp60 include miR-5, miR-994, miR-969, and miR-1011, which jointly play a role. Therefore, microRNAs are critical for normal tick development, and the primary structure of the mature sequence of miR-1 is highly conserved. Nonetheless, different developmental stages and tissues show different expression patterns, with a certain role in prolonging feeding. miR-1, together with other family members, regulates mRNA function and may be used as a molecular marker for species origin and evolution analysis and internal reference gene selection.

In vitro Assessment of Period of Oviposition and Hatching in Hyalomma anatolicum anatolicum

Hyalomma anatolicum anatolocum is one of the most important tick species in India (Geeverghese and Dhanda, 1987), which assumed noticeable importance because of its role as the major vector of the hemoprotozoan parasites. Besides, the role of Hyalomma species in transmitting the Crimean-Congo hemorrhagic fever (CCHF) virus in humans has been established in different countries (Karti et al., 2004). Ticks produce severe economic losses through blood-sucking and acting as vectors of pathogens and toxins. In India alone, the cost of tick and tick-borne diseases (TTBDs) in animals has been estimated to the tune of approx. Two thousand crores (Ghosh et al., 2007). Scanty literature is available pertaining to the life cycle of H. a. anatolicum in general and from Madhya Pradesh in particular. Therefore, the present study was designed to record the period of oviposition and hatching of eggs in H. a. anatolicum.

In vitro Efficacy of Deltamethrin against Hyalomma anatolicum anatolicum

Tick and tick-borne diseases such as theileriosis,babesiosis, and Anaplasmosis pose a constant threato cattle health. Rhipicephalus (Boophilus) microplus andHyalomma anatolicum are the most commonly foundcattle tick species and cause significant economic lossesto dairy and leather industries by adversely affecting themilk production and quality of hides. Tick control strategyinvolves mainly the use of synthetic acaricides which resultsin the development of acaricide resistance. Deltamethrinacts on the parasites by contact and has strong lipophilicaction thus penetrates the cuticle nerve ganglion and block the motor activity. The presentstudy was aimed at the determination of in vitro efficacyof Deltamethrin against H. anatolicum ticks collected fromhealthy cattle of college farm and nearby dairy farms.

Studies on Period of Oviposition and Hatching of Eggs in Hyalomma anatolicum anatolicum

The engorged Hyalomma anatolicum anatolicum females were collected from healthy cattle of college farm as well as nearby private dairy farms and villages of Mhow to evaluate the period of oviposition and hatching of eggs of H. a. anatolicum under laboratory conditions. The present study recorded the period of oviposition and hatching of eggs (Mean ± SE) as 9.4 ± 0.54 and 21.9 ± 0.69 days, respectively.

In vitro efficacy of cypermethrin against Hyalomma anatolicum anatolicum

The present investigation was undertaken to evaluate in vitro efficacy of cypermethrin against H. a.anatolicum. The engorged H. a.anatolicum females dropped on ground were collected from dairy farms of nearby villages of Mhow and were maintained in the laboratory till hatching of the eggs and larvae were used for further study. Larvae were placed in filter paper packets impregnated with 100, 200, 400, and 800 ppm concentrations of cypermethrin for 24 hours which revealed the per cent mortality 89.84, 93.70, 100 and 100%, while the corrected percent mortality and efficacy was found 89.30, 93.36, 100 and 100%

Investigation of ticks infecting sheep and seasonal changes of their population in Eyvan city

<p>Ticks are arthropods that are the most important pests in the veterinary industry, which transmit important and dangerous diseases of humans and animals. Eyvan city located in the northern half of Ilam province and one of the most important centers of agriculture and animal husbandry is the province of Ilam. Hence, it is essential to identify the correct method for struggle tick and study the season's abundance and type of host. This study was carried out at the level of shambles in the city of Eyvan and from the first of April of 2016 to March of 2017. From the 1,500 sheep of different age, 220 viruses (14.66%) were infect with ticks. The average number of ticks in infected sheep was 2.9 ticks per sheep. The results showed that in sheep, 220 (38.66%) were male and 350 (61.40%) were female ticks. The tick identified on the sheep was related to 5 species and 3 ticks. The most frequency was related to the hyalomma genus, which includes <em>Hyalomma asiaticum asiaticum</em> (34.21%), <em>Hyalomma anatolicum anatolicum</em> (19.30%), also, the highest infection rate was in sheep in the city of Eyvan in June. In general, the prevalence and severity of infection were low but a relatively high species diversity was observed.</p>

Prevalence of Ixodid Ticks in Cattle Population of Indore, Madhya Pradesh

A survey study was conducted to evaluate the prevalence of ixodid ticks in cattle population of Indore, Madhya Pradesh. Every month, ticks were collected from 9511 cattle belonging to randomly selected 5 villages of each tehsil, viz. Indore, Mhow, Depalpur, Sanwer and Hatod. The current study revealed an overall tick prevalence of 58.87% with significantly high prevalence of Rhipicephalus (Boophilus) microplus (42.89%) than Hyalomma anatolicum anatolicum (11.82%) and mixed infestation (4.16%). Season-wise, significantly high prevalence was recorded in monsoon (71.08%) than summer (62.28%) and winter (52.12%). Amongst the various age groups, significantly high tick infestation was encountered in 1-3 year-old (78.63%) than >3 year-old (56.79%) and is less than 1 year-old cattle (52.92%). Sex-wise, significantly high tick infestation was documented in males (66.01%) than their female (58.06%) counterparts. Tehsil-wise, significantly high prevalence was evident in Mhow tehsil (69.12%) as compared to Indore (61.40%), Depalpur (57.27%), Sanwer (55.28%) and Hatod (50.14%) tehsils.