Droplet-Based Microfluidic Technology for Cell Analysis

Microfluidic technology has emerged from the MEMS (Micro-Electro-Mechanical System)-technology as an important research field. During the last decade, various microfluidic technologies have been developed to open up a new era for biological studies. To understand the function of single cells, it is very important to monitor the dynamic behavior of a single cell in a living environment. Cell trapping in single cell analysis is urgently demanded There have been some review papers focusing on drug screen and cell analysis. However, cell trapping in single cell analysis has rarely been covered in the previous reviews. The present paper focuses on recent developments of cell trapping and highlights the mechanisms, governing equations and key parameters affecting the cell trapping efficiency by contact-based and contactless approach. The applications of the cell trapping method are discussed according to their basic research areas, such as biology and tissue engineering. Finally, the paper highlights the most promising cell trapping method for this research area.

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Recent advances in single-cell analysis: Encapsulation materials, analysis methods and integrative platform for microfluidic technology

Talanta ◽

10.1016/j.talanta.2021.122671 ◽

2021 ◽

pp. 122671

Author(s):

Jiadi Sun ◽

Lu Gao ◽

Liping Wang ◽

Xiulan Sun

Keyword(s):

Single Cell ◽

Single Cell Analysis ◽

Cell Analysis ◽

Materials Analysis ◽

Analysis Methods ◽

Recent Advances ◽

Microfluidic Technology

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Fabrication and Applications of Microfluidic Devices: A Review

International Journal of Molecular Sciences ◽

10.3390/ijms22042011 ◽

2021 ◽

Vol 22 (4) ◽

pp. 2011

Author(s):

Adelina-Gabriela Niculescu ◽

Cristina Chircov ◽

Alexandra Cătălina Bîrcă ◽

Alexandru Mihai Grumezescu

Keyword(s):

Fluid Dynamics ◽

Cell Culture ◽

Material Science ◽

Biomedical Applications ◽

Microfluidic Devices ◽

Cell Analysis ◽

Microfluidic Chips ◽

Drug Encapsulation ◽

Microfluidic Technology ◽

Nanoparticle Preparation

Microfluidics is a relatively newly emerged field based on the combined principles of physics, chemistry, biology, fluid dynamics, microelectronics, and material science. Various materials can be processed into miniaturized chips containing channels and chambers in the microscale range. A diverse repertoire of methods can be chosen to manufacture such platforms of desired size, shape, and geometry. Whether they are used alone or in combination with other devices, microfluidic chips can be employed in nanoparticle preparation, drug encapsulation, delivery, and targeting, cell analysis, diagnosis, and cell culture. This paper presents microfluidic technology in terms of the available platform materials and fabrication techniques, also focusing on the biomedical applications of these remarkable devices.

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