Design and Characterization of Cold Plasma Ozonator for Wastewater Treatment

Influent characterization and biosorption experiments were carried out with settled influent of seven wastewater treatment plants to study the influence of O2 in the selector in relation to the success of developing good settling properties of the sludge. In previous years working selectors were installed and/or pilot plant research was carried out at these wastewater treatment plants. Characterization of the influent was done with help of standard COD and BOD measurements with help of a coarse filter. The research has elucidated that the presence of O2 in the selector, at initial sludge loadings of 3.5-6.5 kg BOD/kg MLSS.d, is important for producing good settling properties of the sludge when the sum of readily biodegradable COD and rapidly hydrolysable COD is greater than 40%. When the sum of sludge COD and slow hydrolysable COD is greater than 50% an unaerated selector can be used.

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Microstructural characterization of white charcoal for rapid reduction of chemical oxygen demand and automatically adjust pH to neutral in wastewater treatment

Journal of Materials Research and Technology ◽

10.1016/j.jmrt.2021.04.082 ◽

2021 ◽

Author(s):

Nuchanaporn Pijarn ◽

Janpen Intaraprasert ◽

Sukanya Ophap ◽

Thanida Uma ◽

Siripan Deekarnkol ◽

...

Keyword(s):

Wastewater Treatment ◽

Chemical Oxygen Demand ◽

Oxygen Demand ◽

Microstructural Characterization ◽

Rapid Reduction

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Characterization of efficient xylanases from industrial-scale pulp and paper wastewater treatment microbiota

AMB Express ◽

10.1186/s13568-020-01178-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jia Wang ◽

Jiawei Liang ◽

Yonghong Li ◽

Lingmin Tian ◽

Yongjun Wei

Keyword(s):

Wastewater Treatment ◽

Xylanase Activity ◽

Pulp And Paper ◽

Optimal Conditions ◽

Paper Wastewater ◽

Termite Hindgut ◽

Uncultured Microorganisms ◽

Metagenomic Approach ◽

Gh10 Family

AbstractXylanases are widely used enzymes in the food, textile, and paper industries. Most efficient xylanases have been identified from lignocellulose-degrading microbiota, such as the microbiota of the cow rumen and the termite hindgut. Xylanase genes from efficient pulp and paper wastewater treatment (PPWT) microbiota have been previously recovered by metagenomics, assigning most of the xylanase genes to the GH10 family. In this study, a total of 40 GH10 family xylanase genes derived from a certain PPWT microbiota were cloned and expressed in Escherichia coli BL21 (DE3). Among these xylanase genes, 14 showed xylanase activity on beechwood substrate. Two of these, PW-xyl9 and PW-xyl37, showed high activities, and were purified to evaluate their xylanase properties. Values of optimal pH and temperature for PW-xyl9 were pH 7 and 60 ℃, respectively, while those for PW-xyl37 were pH 7 and 55 ℃, respectively; their specific xylanase activities under optimal conditions were 470.1 U/mg protein and 113.7 U/mg protein, respectively. Furthermore, the Km values of PW-xyl9 and PW-xyl37 were determined as 8.02 and 18.8 g/L, respectively. The characterization of these two xylanases paves the way for potential application in future pulp and paper production and other industries, indicating that PPWT microbiota has been an undiscovered reservoir of efficient lignocellulase genes. This study demonstrates that a metagenomic approach has the potential to screen efficient xylanases of uncultured microorganisms from lignocellulose-degrading microbiota. In a similar way, other efficient lignocellulase genes might be identified from PPWT treatment microbiota in the future.

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The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host

FEMS Microbiology Ecology ◽

10.1093/femsec/fiab029 ◽

2021 ◽

Vol 97 (4) ◽

Author(s):

Rute Ferreira ◽

Rui Amado ◽

Jorge Padrão ◽

Vânia Ferreira ◽

Nicolina M Dias ◽

...

Keyword(s):

Wastewater Treatment ◽

Bacterial Communities ◽

Potential Application ◽

Deleterious Effect ◽

Wastewater Treatment Plants ◽

Lytic Phage ◽

Filamentous Bacterium ◽

Sphaerotilus Natans ◽

Isolation And Characterization

ABSTRACT Bacteriophages (phages) are ubiquitous entities present in every conceivable habitat as a result of their bacterial parasitism. Their prevalence and impact in the ecology of bacterial communities and their ability to control pathogens make their characterization essential, particularly of new phages, improving knowledge and potential application. The isolation and characterization of a new lytic phage against Sphaerotilus natans strain DSM 6575, named vB_SnaP-R1 (SnaR1), is here described. Besides being the first sequenced genome of a Sphaerotilus natans infecting phage, 99% of its 41507 bp genome lacks homology with any other sequenced phage, revealing its uniqueness and previous lack of knowledge. Moreover, SnaR1 is the first Podoviridae phage described infecting this bacterium. Sphaerotilus natans is an important filamentous bacterium due to its deleterious effect on wastewater treatment plants (WWTP) and thus, phages may play a role as novel biotechnological tools against filamentous overgrowth in WWTP. The lytic spectrum of SnaR1 was restricted to its host strain, infecting only one out of three S. natans strains and infection assays revealed its ability to reduce bacterial loads. Results suggest SnaR1 as the prototype of a new phage genus and demonstrates its potential as a non-chemical alternative to reduce S. natans DSM 6575 cells.

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Detection and molecular characterization of enteric adenovirus in treated wastewater in the Brazilian Federal District

SN Applied Sciences ◽

10.1007/s42452-021-04678-2 ◽

2021 ◽

Vol 3 (7) ◽

Author(s):

T. S. C. Quintão ◽

F. G. Silva ◽

A. L. Pereira ◽

W. N. Araújo ◽

P. M. Oliveira ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Wastewater Treatment ◽

Wastewater Treatment Plants ◽

Federal District ◽

Treated Wastewater ◽

Conventional Pcr ◽

Rainfall Index ◽

Enteric Adenovirus ◽

Enteric Adenoviruses

AbstractHuman enteric viruses, such as enteric adenoviruses (HAdV), are known to be involved with gastrointestinal disorders, especially acute gastroenteritis. Several studies have used HAdV as an indicator of water quality, since they are considered highly stable and widely distributed viruses in water matrices. The aim of this study was to detect and genotype HAdVs in water matrices impacted by discharges of treated effluents from wastewater treatment plants (WWTPs). Wastewater treatment plants from the sanitary system of the Brazilian Federal District were assessed in 2018 and 2019. Samples were collected upstream and downstream from discharge points for each WWTP. Viral concentration based on adsorption-elution and conventional PCR was used for molecular detection, and positive samples were sequenced for phylogenetic analysis. Pluviosity data for the period in which the samples were collected were obtained. Our results demonstrated the presence of HAdVs in 27.2% (61/224) of the samples. The positivity was significantly higher in downstream samples compared to upstream. Moreover, the HAdV positivity was higher in downstream samples collected from receiving water bodies impacted by secondary-level WWTPs in comparison with those impacted by tertiary-level WWTPs. Phylogenetic analysis demonstrated the presence of genotypes 40 and 41, with prevalence of HAdV genotype 41. Despite the predominance of HAdV-41, an increasing frequency of the HAdV-40 was associated with higher pluviosity. In conclusion, this study is the first documentation in the Brazilian Federal District dealing with the prevalence and diversity of HAdVs in several WWTP, along with their correlation with rainfall index.

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LAYERED DOUBLE HYDROXIDES: NANOMATERIALS FOR APPLICATIONS IN AGRICULTURE

Revista Brasileira de Ciência do Solo ◽

10.1590/01000683rbcs2015081 ◽

2015 ◽

Vol 39 (1) ◽

pp. 1-13 ◽

Cited By ~ 39

Author(s):

Luíz Paulo Figueredo Benício ◽

Rejane Alvarenga Silva ◽

Júnia Aparecida Lopes ◽

Denise Eulálio ◽

Rodrigo Morais Menezes dos Santos ◽

...

Keyword(s):

Wastewater Treatment ◽

Layered Double Hydroxides ◽

Slow Release ◽

Structural Characteristics ◽

Synthesis Methods ◽

Double Hydroxides ◽

Near Future

The current research aims to introduce Layered Double Hydroxides (LDH) as nanomaterials to be used in agriculture, with particular reference to its use as storage and slow release matrix of nutrients and agrochemicals for plant growing. Structural characteristics, main properties, synthesis methods and characterization of LDH were covered in this study. Moreover, some literature data have been reported to demonstrate their potential for storage and slow release of nitrate, phosphate, agrochemicals, besides as being used as adsorbent for the wastewater treatment. This research aims to expand, in near future, the investigation field on these materials, with application in agriculture, increasing the interface between chemistry and agronomy.

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Estimation of Kinetic Parameters of Heterotrophic Biomass under Aerobic Conditions and Characterization of Wastewater for Activated Sludge Modelling

Water Science & Technology ◽

10.2166/wst.1992.0118 ◽

1992 ◽

Vol 25 (6) ◽

pp. 125-139 ◽

Cited By ~ 174

Author(s):

J. Kappeler ◽

W. Gujer

Keyword(s):

Wastewater Treatment ◽

Activated Sludge ◽

Kinetic Parameters ◽

Specific Growth ◽

Wastewater Treatment Plants ◽

Simple Method ◽

Batch Tests ◽

Different Types ◽

Heterotrophic Biomass

To predict the behaviour of biological wastewater treatment plants, the Activated Sludge Model No. 1 is often used. For the application of this model kinetic parameters and wastewater composition must be known. A simple method to estimate kinetic parameters of heterotrophic biomass and COD wastewater fractions is presented. With three different types of batch-tests these parameters and fractions can be determined by measuring oxygen respiration. Our measurements showed that the maximum specific growth rate µmax of heterotrophic biomass depends on temperature, reactor configuration and SRT. In typical wastewater treatment plants of Switzerland the amount of readily biodegradable substrate was generally small (about 9 % of the COD in primary effluent). The same method can also be used to determine kinetic parameters of nitrifying biomass.

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