Analysis of Low Molecular Proteins Obtained from Human Placental Extract Considered as New Strategic Biomaterial for Pulp-Dentinal Regeneration

Abstract:: The placenta that maintains and regulates the growth of fetus, consists of various biological treasures nutrients such as cytomedines, vitamins, trace elements, amino acids, peptides, growth factors and other biologically active constituents. Their therapeutic usefulness can well define in the terms of biochemical mechanisms of various components present in it. Biomedical waste derived extract is also a panacea for treatment of various diseases. Placental therapy has been reported specifically to have potent action on recovery of diseases and tissue regeneration. Placental bioactive components and their multi targeting identity prompted us to compile the précised information on placental extract products. However, some findings are needed to be explored by scientific community to prove their clinical potential with clinically significant statistical conclusions. In the light of available information and the usefulness of the placental extract, it is necessary for the development of various formulations for various unmet meet for the treatment as well as access their adverse effects as well as contradictions and precisely evaluated in the short and in the long-term periods.

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Inhibition of Prolactin Secretion of Rat Placental Extract

Experimental Biology and Medicine ◽

10.3181/00379727-172-41521 ◽

1983 ◽

Vol 172 (1) ◽

pp. 29-33

Author(s):

R. F. Laherty ◽

J. M. Budd ◽

H. H. Srebnik

Keyword(s):

Prolactin Secretion ◽

Placental Extract

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Effect of Placental Extract on Omentum Mesenchymal Stem Cells Differentiation in NMRI Mice

Journal of Molecular Biology Research ◽

10.5539/jmbr.v7n1p176 ◽

2017 ◽

Vol 7 (1) ◽

pp. 176

Author(s):

Maryam Sadat Nezhadfazel ◽

Kazem Parivar ◽

Nasim Hayati Roodbari ◽

Mitra Heydari Nasrabadi

Keyword(s):

Cell Cycle ◽

Stem Cells ◽

Flow Cytometry ◽

Endothelial Cells ◽

Mesenchymal Stem Cells ◽

Control Group ◽

Fat Cell ◽

Nmri Mice ◽

Differentiated Cells ◽

Placental Extract

Omentum mesenchymal stem cells (OMSCs) could be induced to differentiate into cell varieties under certain conditions. We studied differentiation of OMSCs induced by using placenta extract in NMRI mice. Mesenchymal stem cells (MSCs) were isolated from omentum and cultured with mice placenta extract. MSCs, were assessed after three passages by flow cytometry for CD90, CD44, CD73, CD105, CD34 markers and were recognized their ability to differentiate into bone and fat cell lines. Placenta extract dose was determined with IC50 test then OMSCs were cultured in DMEM and 20% placenta extract.The cell cycle was checked. OMSCs were assayed on 21 days after culture and differentiated cells were determined by flow cytometry and again processed for flow cytometry. CD90, CD44, CD73, CD105 markers were not expressed, only CD34 was their marker. OMSCs were morphologically observed. Differentiated cells are similar to the endothelial cells. Therefore, to identify differentiated cells, CD31 and FLK1 expression were measured. This was confirmed by its expression. G1 phase of the cell cycle shows that OMSCs compared to the control group, were in the differentiation phase. The reason for the differentiation of MSCs into endothelial cells was the sign of presence of VEGF factor in the medium too high value of as a VEGF secreting source.

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Specificity of a placental factor inhibiting breathing in fetal sheep

Reproduction Fertility and Development ◽

10.1071/rd9960423 ◽

1996 ◽

Vol 8 (3) ◽

pp. 423 ◽

Cited By ~ 3

Author(s):

RE Alvaro ◽

V Rehan ◽

Almeida V de ◽

Z Haider ◽

M Robertson ◽

...

Keyword(s):

High Voltage ◽

Low Voltage ◽

Fetal Liver ◽

Fetal Life ◽

Krebs Solution ◽

Fetal Sheep ◽

Electrocortical Activity ◽

Fetal Breathing ◽

Pregnant Ewe ◽

Placental Extract

We have found previously that the infusion of a placental extract inhibits breathing induced by 100% O2 plus umbilical cord occlusion in the fetal sheep, suggesting that a placental factor is responsible for the inhibition of fetal breathing. To test whether this factor is specific to the placenta and whether it also inhibits spontaneous fetal breathing (occurring in the absence of cord occlusion), we administered extracts from the placenta, muscle and liver of the pregnant ewe, extracts of fetal liver, and Krebs solution to 16 chronically instrumented fetal sheep at 135 +/- 5 days of gestation. Infusions were made during low-voltage electrocortical activity, 5 to 15 min after a switch from high voltage, when breathing was well established. Within 90 s of the infusion of the placental extract in the carotid artery of the fetus, breathing decreased in 79% (33/42) of the experiments and was completely abolished in 71% (30/42) of them (P < 0.0001 compared with the other infusates). No apnoeas were observed with the Krebs solution (0/19) and the maternal muscle (0/20). Extracts of maternal and fetal liver abolished breathing in only 17% (4/23) and 21% (6/29) of the experiments respectively (NS compared with Krebs solution). There were no significant changes in blood gas tensions, pH, blood pressure and heart rate associated with the infusion of the extracts. The electrocortical activity (ECoG) switched from low to high voltage in 50% of the experiments using placental extract compared with 0% with Krebs solution and maternal muscle, and with 9% and 17% with maternal and fetal liver respectively (P < 0.005). Breathing output (integral of EMGdi x f) during and after the infusions significantly decreased only with the placental extract. These findings indicate the presence of a factor produced by the placenta which inhibits fetal breathing and may be responsible for the normal inhibition of breathing observed in fetal life.

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Extraction and characterization of yeast extract bioethanol byproduct from empty palm oil bunch for raw material of cosmetic products

E3S Web of Conferences ◽

10.1051/e3sconf/20186703040 ◽

2018 ◽

Vol 67 ◽

pp. 03040

Author(s):

Muhamad Sahlan ◽

Muhammad Saefuddin ◽

Muryanto ◽

Heri Hermansyah ◽

Anondho Wijanarko

Keyword(s):

Yeast Extract ◽

Raw Materials ◽

Raw Material ◽

Yeast Fermentation ◽

Yeast Saccharomyces Cerevisiae ◽

Chromatography Method ◽

Column Adsorption ◽

Liquid Chromatography Method ◽

Feasibility Test ◽

Placental Extract

Ethanolic fermentation can produce byproducts such as yeast containing intracellular amino acid that is used as a raw material of cosmetics. Residual yeast fermentation as sludge was dissolved and extracted by autolysis at 50°C for 24 hours, so we get the product in the form of intracellular content of the yeast Saccharomyces cerevisiae. Purification of dye and odor yeast extract was conducted by using an activated carbon column adsorption with ratio 1.5:10 yeast extract solution (g / mL) for six times recycle or until it reaches the absorbance value of 0.020. The content of yeast extract in the form of amino acids was analyzed by High-Pressure Liquid Chromatography method. Analysis of the feasibility test yeast extract as cosmetic raw materials made through the pigment deposition method by inhibit tyrosinase activity. 0.05 g yeast extract before adsorption (pale yellow) produce 62% inhibition of tyrosinase 3130 U / mL. Dry yeast extract after adsorption (odorless) had 96% inhibition of tyrosinase 313 U / mL, whereas placental extract by 89% inhibition of tyrosinase 313 U / mL. These results indicate odorless yeast extract can replace placental extract as an alternative to cosmetic raw materials.

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Radioimmunoassay of inhibin based on synthetic human inhibin alpha-chain peptide

Clinical Chemistry ◽

10.1093/clinchem/37.1.40 ◽

1991 ◽

Vol 37 (1) ◽

pp. 40-46 ◽

Cited By ~ 1

Author(s):

M J Sinosich ◽

S Sieg ◽

A Zakher ◽

N Ling ◽

D M Saunders ◽

...

Keyword(s):

Specific Activity ◽

Biological Fluids ◽

Mammalian Species ◽

Protein A ◽

Divalent Metal Cations ◽

Polyethylene Glycol 200 ◽

Separation Phase ◽

Ovarian Inhibin ◽

Placental Extract

Abstract Polyclonal rabbit antisera were produced against cyclic human inhibin [(Cys6, Tyr7) alpha-(6-30)NH2] peptide, covalently conjugated to bovine serum albumin. The tyrosine residue introduced at position 7 facilitated the oxidative incorporation of radiolabel (125I) to yield a tracer with specific activity of 73.9 Ci/g. These reagents were used to develop a homologous equilibrium radioimmunoassay for human inhibin, with polyethylene glycol, 200 g/L, serving as the separation phase. At a detection limit of 2 micrograms/L (n = 7), immunoactive inhibin was detectable in human pre-ovulatory follicular fluid (128 micrograms/L), seminal plasma (2374 micrograms/L), amniotic fluid (66 micrograms/L), and placental extract (347 micrograms/L). We also demonstrated inhibin immunoreactivity in biological fluids from other mammalian species: macaque, chimpanzee, porcine, and bovine, but not rodent (guinea pig). Although the antisera were raised against a nonbioactive inhibin peptide, immunoglobulins fractionated on Protein A-Sepharose neutralized the bioactivity of human ovarian inhibin. Further characterization of inhibin immuno- and bioactivity was undertaken with immobilized heparin, divalent metal cations, and dye ligands. Only heparin-Sepharose distinguished between immuno- and bioactive inhibin.

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