scholarly journals Extraction and characterization of yeast extract bioethanol byproduct from empty palm oil bunch for raw material of cosmetic products

2018 ◽  
Vol 67 ◽  
pp. 03040
Author(s):  
Muhamad Sahlan ◽  
Muhammad Saefuddin ◽  
Muryanto ◽  
Heri Hermansyah ◽  
Anondho Wijanarko
Keyword(s):  
Yeast Extract ◽  
Raw Materials ◽  
Raw Material ◽  
Yeast Fermentation ◽  
Yeast Saccharomyces Cerevisiae ◽  
Chromatography Method ◽  
Column Adsorption ◽  
Liquid Chromatography Method ◽  
Feasibility Test ◽  
Placental Extract

Ethanolic fermentation can produce byproducts such as yeast containing intracellular amino acid that is used as a raw material of cosmetics. Residual yeast fermentation as sludge was dissolved and extracted by autolysis at 50°C for 24 hours, so we get the product in the form of intracellular content of the yeast Saccharomyces cerevisiae. Purification of dye and odor yeast extract was conducted by using an activated carbon column adsorption with ratio 1.5:10 yeast extract solution (g / mL) for six times recycle or until it reaches the absorbance value of 0.020. The content of yeast extract in the form of amino acids was analyzed by High-Pressure Liquid Chromatography method. Analysis of the feasibility test yeast extract as cosmetic raw materials made through the pigment deposition method by inhibit tyrosinase activity. 0.05 g yeast extract before adsorption (pale yellow) produce 62% inhibition of tyrosinase 3130 U / mL. Dry yeast extract after adsorption (odorless) had 96% inhibition of tyrosinase 313 U / mL, whereas placental extract by 89% inhibition of tyrosinase 313 U / mL. These results indicate odorless yeast extract can replace placental extract as an alternative to cosmetic raw materials.

scholarly journals Conversion Characteristics of Some Major Cannabinoids from Hemp (Cannabis sativa L.) Raw Materials by New Rapid Simultaneous Analysis Method

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4113
Author(s):  
Byeong Ryeol Ryu ◽  
Md. Jahirul Islam ◽  
Md. Obyedul Kalam Azad ◽  
Eun-Ji Go ◽  
Md. Hafizur Rahman ◽  
...  
Keyword(s):  
High Performance ◽  
Cannabis Sativa ◽  
Raw Materials ◽  
Raw Material ◽  
Natural State ◽  
Gradient System ◽  
Uv Spectrum ◽  
Chromatography Method ◽  
Advanced Analysis ◽  
Liquid Chromatography Method

This study was carried out to develop a high-performance liquid chromatography method for short-time analysis of the main cannabinoids in the inflorescence of hemp (Cannabis sativa L.). We also performed decarboxylation of the raw material using our advanced analysis technique. In this study, the UV spectrum was considered to analyze each of the four common cannabinoids, solvents, and samples, where the uniform elution of acidic cannabinoids without peak tailing and acids was tested. Optimal results were obtained when readings were taken at a wavelength of 220 nm using water and methanol containing trifluoroacetic acid as mobile phases in a solvent gradient system. The established conditions were further validated by system suitability, linearity, precision, detection limit, and quantitation limit tests. The decarboxylation index (DT50) confirmed that Δ9-THCA decarboxylated faster than CBDA, and both maintained a linear relationship with time and temperature. In addition, the loss of cannabidiol was better prevented during the decarboxylation process in the natural state than in the extracted state.

scholarly journals New Liquid Chromatography Method with Ultraviolet Detection for Analysis of Anthocyanins and Anthocyanidins in Vaccinium myrtillus Fruit Dry Extracts and Commercial Preparations

2007 ◽  
Vol 90 (4) ◽  
pp. 911-919 ◽  
Author(s):  
Ciro Cassinese ◽  
Eric De Combarieu ◽  
Mario Falzoni ◽  
Nicola Fuzzati ◽  
Roberto Pace ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Raw Materials ◽  
High Specificity ◽  
Pharmaceutical Products ◽  
Vaccinium Myrtillus ◽  
Vision Disorders ◽  
Chromatography Method ◽  
Liquid Chromatography Method ◽  
Materials Used ◽  
High Degree

Abstract The Vaccinium myrtillus fruits (bilberry) are a well-known anthocyanins source, and their extracts are widely used in dietary botanicals and pharmaceutical products for the treatment of vascular and vision disorders. Different analytical methods used for standardization of the bilberry extracts and their preparations are available from pharmacopeias and from the literature. However, the methods reported in the literature do not allow the detection of free anthocyanidins, which are markers of poor product quality. A new liquid chromatography method was developed and validated for the identification and quantification of both anthocyanins and anthocyanidins present in bilberry extracts and products. The method shows a good reproducibility and, due to its high specificity, is suitable to identify unequivocally the botanical raw materials used for manufacturing and to evaluate the extract composition, thus ensuring a high degree of product consistency and quality. Forty typical bilberry preparations belonging to 24 different brands were purchased in the marketplace and evaluated for their quality by using the developed method. Results revealed marked differences among the brands despite a common origin and labeling.

scholarly journals BIOTECHNOLOGY OF ALCOHOLIC FERMENTATION WITH YEAST RECIRCULATION

2019 ◽  
Vol 13 (3) ◽  
Author(s):  
L. Levandovsky ◽  
O. Vitriak ◽  
M. Demichkovska
Keyword(s):  
Alcoholic Fermentation ◽  
Anaerobic Fermentation ◽  
Raw Materials ◽  
Initial Period ◽  
Fuel Ethanol ◽  
Raw Material ◽  
Secondary Products ◽  
Yeast Saccharomyces Cerevisiae ◽  
Industrial Environment ◽  
Materials Used

In recent decades, there has been a tendency in the world to increase ethanol production significantly in order to solve energy problems, that is, to use it as a biofuel. The factors determining the production cost of targeted biotechnological products include the output of these products from the raw materials used. One of the modern and effective ways to intensify alcoholic fermentation and reduce the cost of fuel ethanol is yeast recirculation. The research objects were: raw material (sugarbeet molasses), molasses wort, yeast Saccharomyces cerevisiae of the strain M-5, fermented wash and its distillates. In the raw materials, intermediate products, and fermented wash, the techno-chemical parameters recommended by the current technology regulations for obtaining spirit from molasses have been determined. Acoholic fermentation was carried out in an industrial environment, in a battery of series-connected fermentors. Recirculation of yeast was carried out by separating it from the final stage of fermentation, concentrating it on the separator, and introducing it into the first fermentor. The experimental data obtained prove that for wort fermentation, it is effective to use yeast that recirculates in the anaerobic stage. It has been established that the alcohol-forming power of recycled yeast increases as the yeast adapts to the environment in which it has been staying for a long time. The yeast becomes more active biochemically, with more efficient metabolism. Its need for continuously cultured biomass is reduced, the share of aerobically assimiliated sugars decreases, and, consequently, the losses during yeast generation are fewer. At the same time, accelerating the initial period of anaerobic fermentation helps inhibit the biosynthesis of glycerol, the formation of which consumes the largest amount of sugar among all the secondary products. The parameters of molasses wort fermentation, with yeast biomass recirculating, have been determined in an industrial environment. It has been established that the alcohol output from the raw materials increases as the synthesis of secondary metabolic products weakens. The advantages of this fermentation method will be used in further studies, namely when fermenting molasses wort, with an increased concentration of dry matter, in order to reduce the specific heat energy consumption in production and to make it cheaper. The developed biotechnology of alcohol can be usefully employed to produce fuel ethanol, and increasing its production will contribute to Ukraine’s energy self-sufficience.

Isolation and Determination of Phenolic Glycosides and Anthraquinones from Rhizomes of Various Reynoutria Species

Planta Medica ◽  
2018 ◽  
Vol 84 (15) ◽  
pp. 1118-1126 ◽  
Author(s):  
Izabela Nawrot-Hadzik ◽  
Sebastian Granica ◽  
Krzysztof Domaradzki ◽  
Łukasz Pecio ◽  
Adam Matkowski
Keyword(s):  
High Performance ◽  
Raw Materials ◽  
Reversed Phase ◽  
Array Detector ◽  
Sucrose Esters ◽  
Chromatography Method ◽  
Reynoutria Japonica ◽  
Flight Mass Spectrometry ◽  
Liquid Chromatography Method ◽  
Reynoutria Sachalinensis

AbstractGiant knotweeds of the genus Reynoutria (syn. Fallopia)–Reynoutria japonica, Reynoutria sachalinensis, and a hybrid of them, Reynoutria x bohemica–are noxious invasive plants in Europe and North America. R. japonica is a traditional East Asian (Japan and China) drug (Polygoni cuspidati rhizoma). Recently, it has been included in European Pharmacopoeia as one of the traditional Chinese medicinal herbs. In this study, a reversed-phase high performance liquid chromatography method with diode array detector and time-of-flight mass spectrometry was developed and validated for the profiling of rhizomes from European invasive populations and Polygoni cuspidati rhizoma purchased in China. Twenty-five compounds were identified, mainly stilbenes, anthraquinones, flavan-3-ols, and phenylpropanoid esters. Tatariside B, hydropiperoside, vanicoside C, a new compound (3,6-O-di-p-coumaroyl)-β-fructofuranosyl-(2 → 1)-(2′-O-acetyl-6′-O-feruloyl)-β-glucopyranoside) were reported for the first time in these raw materials. Six compounds from three phytochemical classes–stilbenes: piceid and resveratrol; anthraquinones: emodin and physcion; hydroxycinnamic sucrose esters: vanicosides A and B–were quantified using the validated method. R. japonica from China contained twice as many stilbenoids than samples from Poland (piceid 14.83 mg/g dm vs. 7.45 mg/g and resveratrol 1.29 mg/g vs. 0.65 mg/g). R. sachalinensis rhizomes contained lower quantities of anthraquinones and no detectable stilbenes, which together with higher amounts of hydroxycinnamic glycosides makes it easily distinguishable from the other two. The phytochemical profile of R. x bohemica was intermediate between the two parent species.

scholarly journals Forced degradation studies and stability-indicating liquid chromatography method for determination of tirofiban hydrochloride and synthetic impurities

2020 ◽  
Vol 49 (2) ◽  
Author(s):  
Adriane Lettnin Roll Feijó ◽  
Fernanda Macke Hellwig ◽  
Clésio Soldateli Paim ◽  
Marcelo Donadel Malesuik
Keyword(s):  
Liquid Chromatography ◽  
Degradation Products ◽  
Gradient Elution ◽  
Pharmaceutical Products ◽  
Raw Material ◽  
Forced Degradation ◽  
Chromatography Method ◽  
Stability Indicating ◽  
Liquid Chromatography Method

This study aimed to develop and validate a stability-indicating liquid chromatography method for the determination of tirofiban hydrochloride and two synthetic impurities (impurity A and impurity C). The method utilizes a RP-18 column (250 mm × 4.6 mm; 5 μm) with the PDA detector for quantitation. A mixture of triethylamine 0.1% (acidified to pH 5.5 with phosphoric acid) and acetonitrile was used as the mobile phase at a flow rate of 1 mL min−1 with gradient elution. The method presented satisfactory linearity, precision, accuracy and robustness, as well as low limits of detection and quantification, which demonstrate sensitivity in the determination of tirofiban and impurities A and C. It was selective for the determination of the drug and impurities analysed, without interference of the degradation products generated under forced conditions, demonstrating the stability-indicating capacity of the proposed method. Tirofiban showed to be practically stable to oxidative (30% H2O2 for 24 h) and thermal (75 ºC for 24 h) conditions, but presented degradation to UVA light and acid hydrolysis, obeying the first order kinetics for both. In this way, it can be used as a stability-indicating method in the quality control of the raw material of tirofiban hydrochloride, as well as of the finished product. The obtained results demonstrate the importance of deepening the studies in this area, in order to guarantee the quality of commercialized pharmaceutical products.

scholarly journals QUANTITATIVE DETERMINATION OF ARTEANNUIN B – A SESQUITERPENE LACTONE FROM ARTEMISIA ANNUA

2021 ◽  
pp. 163-170
Author(s):  
Rimma Fail'yevna Mukhamatkhanova ◽  
Vyacheslav Vadimovich Uzbekov ◽  
Dilnoza Karimberdievna Mutalova ◽  
Munirakhon Akhmatkhon Kizi Mamatkhanova ◽  
Il'dar Dzhamil'yevich Sham’yanov ◽  
...  
Keyword(s):  
High Performance ◽  
Standard Sample ◽  
Separation Efficiency ◽  
Artemisia Annua ◽  
Raw Material ◽  
Chromatography Method ◽  
Marker Compound ◽  
Liquid Chromatography Method ◽  
System Separation

The HPLC (High performance liquid chromatography) method for the determination of arteannuin B in the herb Artemisia annua has been developed. The method is suitable for its validation characteristics, such as specificity parameters (convergence of the retention times of artianuin B in the standard and sample), suitability of the chromatographic system (separation efficiency), linearity (R2 = 0.99929 at y = 9.38x + 245.98), range of application (from 50 to 150%), accuracy in terms of repeatability (RSD = 3.45%) and correctness (Z = 100.36 ± 0.56%), determined experimentally, are within the recommended values. As a marker compound, it was proposed to use a standard sample of arteannuin B. For this, a standard sample of arteannuin B was obtained with a purity of at least 99%, which is confirmed IR, 1HNMR, 13CNMR spectral data. Organoleptic and physicochemical parameters were determined. It has been established that the presence of impurities in an amount of not more than 1% is allowed in a standard sample of arteannuin B. It was established that the content of arteannuin B in the raw material should be at least 0.2%. The development and validation HPLC-UV method for the determination of arteannuin B have to used to standardization the herb of Artemisia annua.

scholarly journals Research of the chemical composition of Artemisia tschernieviana Bess. by gas chromatography method with mass detection

2021 ◽  
Vol 14 (3) ◽  
pp. 282-286
Author(s):  
O. I. Panasenko ◽  
V. I. Mozul ◽  
O. M. Denysenko ◽  
I. I. Aksonova ◽  
V. V. Holovkin
Keyword(s):  
Chemical Composition ◽  
Propionic Acid ◽  
Succinic Anhydride ◽  
Raw Materials ◽  
Raw Material ◽  
Inflammatory Activity ◽  
Mass Detection ◽  
Chromatography Method ◽  
Mass Spectrometric Detector ◽  
Anti Inflammatory

The aim of the work was to study chromato-mass spectroscopic of Artemisia tschernieviana Bess. grass and to establish possible prospects for the use of this plant in medical practice. Materials and methods. Artemisia tschernieviana Bess. grass was chosen as the object of research. Raw materials were collected in July 2021 in the flowering phase (Zaporizhzhia). The tincture was obtained by maceration and the raw material was extracted with methyl alcohol at room temperature for 10 days according to the method of making tinctures. The study of the chemical composition of Artemisia tschernieviana Bess. Grass was carried out using gas chromatograph Agilent 7890B GC System (Agilent, Santa Clara, CA, USA) with mass spectrometric detector Agilent 5977 BGC/MSD (Agilent, Santa Clara, CA, USA) and chromatographic column DB-5ms (30 m × 250 μm × 0.25 μm). Results. During the study it was found that the composition of Artemisia tschernieviana Bess. grass includes 21 compounds (2 in the isomeric state). β-myrcene (17.22 %), sabinene (14.54 %), 2-(4a,8-dimethyl-7-oxo-1,2,3,4,4a,7-hexahydronaphthalen-2-yl) propionic acid (11.57 %), 2-dodecen-1-yl-succinic anhydride (10.84 %) and chamazulene (9.02 %) accounted for the largest percentage. According to the literature review, myrcene has antibacterial, anesthetic and analgesic effects. Sabinene is characterized by antibacterial action. 2-(4a,8-dimethyl-7-oxo-1,2,3,4,4a,7-hexahydronaphthalen-2-yl)-propionic acid is a fatty acid with possible antibacterial and anti-inflammatory activity. 2-dodecen-1-yl-succinic anhydride is used to produce anhydride-modified form of insulin that can be used in the treatment of diabetes. Hamazulen has anti-inflammatory activity. Conclusions. In view of all the above, we can conclude that Artemisia tschernieviana Bess. grass can be recommended for further research as a source of potential anti-inflammatory and antibacterial agents.

scholarly journals Development and Validation of a Column High-Performance Liquid Chromatography Method for Quantification of Ganaphaliin A and B in Inflorescences of Gnaphalium liebmannii Sch. Bp ex Klatt

2011 ◽  
Vol 94 (4) ◽  
pp. 1076-1081 ◽  
Author(s):  
Fernando Rodríguez-Ramos ◽  
Víctor H Sánchez-Estrada ◽  
Alejandro Alfaro-Romero ◽  
Gabriela Rubí Tapia-Álvarez ◽  
Andrés Navarrete
Keyword(s):  
High Performance ◽  
Hplc Method ◽  
Raw Material ◽  
Array Detector ◽  
Chromatography Method ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Liquid Chromatography Method ◽  
Development And Validation

Abstract An HPLC method was developed for the simultaneous determination of gnaphaliin A and B, active compounds of Gnaphalium liebmannii Sch. Bp ex Klatt. The HPLC separation was performed on an Inertsil ODS-3 (150 × 4.6 mm id, 5 μm) RP C18 column operated at 40°C; the isocratic mobile phase was 0.02% aqueous orthophosphoric acid– methanol–acetonitrile (50 + 30 + 20, v/v/v), with a run time of 20 min and flow rate of 1.5 mL/min. Detection with a photodiode array detector (PDAD) was at 270 nm. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ for gnaphaliin A and B were found to be in the range of 0.4–0.5 and 1.0–1.4 μg/mL, respectively. This is the frst report of an analytical method developed for the quantitative analysis of flavones from Gnaphalium species by HPLC-PDAD with applications for raw material and commercial products.

scholarly journals Digestibility of nutrients, feed conversion and living mass gain at adding enzyme compositions based on cellulase and amylase to the compound feed

1970 ◽  
pp. 194-204
Author(s):  
L.P. Chornolata ◽  
V.Y. Novakovska
Keyword(s):  
Raw Materials ◽  
Average Daily Gain ◽  
Mass Gain ◽  
Metabolic Energy ◽  
Laboratory Research ◽  
Feed Conversion ◽  
Immunological Parameters ◽  
Chromatography Method ◽  
Enzyme Preparations ◽  
Liquid Chromatography Method

Purpose. Creation of enzyme preparations, composed of amylase and cellulase and their use in rations of fattening pigs, aimed at increasing productive action and digestibility of the main nutrients of feed. Methods. In the process of the research general scientific methods were used: deduction, scientific hypothesis and abstraction. Specific methods were used for the laboratory research: zootechnical analysis, liquid chromatography method, spectrophotometry, titrometry, balance experiments were performed as well. Methods of variation statistics and the program Statistica 7.0 were used to process the obtained experimental data. Results. The best results were revealed in the fourth balance experiment. It was found that in terms of productivity, the consumption of 5 g of cellulase and 1 g of amylase in the main diet, increases the average daily gain of animals by 21.5% (P<0,01) compared to the control. The level of feed conversion increased due to a decrease in the costs of digestible protein by 19.4%, IVF and metabolic energy – by 4.1%. Thus, the coefficients of digestibility of dry matter increased by 6%, organic matter by – 5.7 %, crude protein by – 5.7% (P<0,05), crude fat – by 11.7%, crude fiber – by 32% (P<0,05), nitrogen-free extractives – by 3.3% compared to the control. Conclusions. Based on the increase in productivity and increase in the level of digestibility of feed nutrients, the efficiency and optimal norms of amylase and cellulase-based enzymes introduction have been established. The use of new enzyme supplements for pigs feeding made from domestic raw materials and the study of the effect of enzyme composition in the dosage of 5 g of cellulase and 1 g of amylase per 1 kg of feed on productive, nutritional, clinical, morphological, immunological parameters of pigs requires further study.

scholarly journals Ethanol is a strategic raw material

2002 ◽  
Vol 56 (3) ◽  
pp. 89-104 ◽  
Author(s):  
Josip Baras ◽  
Slobodan Gacesa ◽  
Dusanka Pejin
Keyword(s):  
Ethanol Production ◽  
Raw Materials ◽  
Motor Fuel ◽  
Review Article ◽  
Raw Material ◽  
Yeast Fermentation ◽  
Bioethanol Production ◽  
Renewable Raw Material ◽  
General Data ◽  
Further Development

The first part of this review article considers general data about ethanol as an industrial product, its qualities and uses. It is emphasized that, if produced from biomass as a renewable raw material, its perspectives as a chemical raw material and energent are brilliant. Starchy grains, such as corn, must be used as the main raw materials for ethanol production. The production of bioethanol by the enzyme-catalyzed conversion of starch followed by (yeast) fermentation, distillation is the process of choice. If used as a motor fuel, anhydrous ethanol can be directly blended with gasoline or converted into an oxygenator such as ETBE. Finally, bioethanol production in Yugoslavia and the possibilities for its further development are discussed.

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