Callus Culture Systems in Salix L.: The Limited Database

2020 ◽  
pp. 83-91
Author(s):  
Afsheen Shahid ◽  
Mohammad Anis
Keyword(s):  
Callus Culture ◽  
Culture Systems

Alterations of ultrastructure and elemental composition in cultured human epidermal keratinocytes after treatment with nitrofurazone and silver sulfadiazine

1987 ◽  
Vol 45 ◽  
pp. 676-677
Author(s):  
A. R. Crooker ◽  
M. C. Myers ◽  
T. L. Beard ◽  
E. S. Graham
Keyword(s):  
Electron Microscopy ◽  
Elemental Composition ◽  
Pyrolytic Carbon ◽  
Human Keratinocytes ◽  
Silver Sulfadiazine ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Culture Systems ◽  
Cytotoxicity Endpoints

Cell culture systems have become increasingly popular as a means of screening toxic agents and studying toxic mechanisms of drugs and other chemicals at the cellular and subcellular levels. These in vitro tests can be conducted rapidly in a broad range of relevant mammalian culture systems; a variety of biological and biochemical cytotoxicity endpoints can be examined. The following study utilized human keratinocytes to evaluate the relative cytotoxicities of nitrofurazone (NF) and silver sulfadiazine (SS), the active ingredients of FURACIN(R) Topical Cream and SILVADENE(R) Cream, respectively. These compounds are anti-infectives used in the treatment of burn patients. Cell ultrastructure and elemental composition were utilized as cytotoxicity endpoints.Normal Human Epidermal Keratinocytes (HK) were prepared from the EpiPackTM culture system (Clonetics Corporation, Boulder, CO). For scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cells were seeded on sterile 35 mm Falcon plastic dishes; for elemental microanalysis, cells were plated on polished pyrolytic carbon discs (E. Fullam, Latham, NY) placed in the culture dishes.

1421: The Fidelity of Cellular Differentiation in Popular Prostate Development Culture Systems

2005 ◽  
Vol 173 (4S) ◽  
pp. 385-385
Author(s):  
Jason Doles ◽  
Robert Lipinski ◽  
Wade Bushman
Keyword(s):  
Cellular Differentiation ◽  
Culture Systems ◽  
Prostate Development

Antioxidant and antihyperlipidemic activity of Tephrosia purpurea callus culture

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
M Mujeeb ◽  
M Aqil ◽  
AK Najmi ◽  
M Akhtar ◽  
N Ahmad ◽  
...  
Keyword(s):  
Callus Culture ◽  
Tephrosia Purpurea ◽  
Antihyperlipidemic Activity

Use of 3D culture systems to generate human induced pluripotent stem cell-derived [beta]-cells in vitro

2018 ◽  
Author(s):  
Fantuzzi Federica ◽  
Toivonen Sanna ◽  
Schiavo Andrea Alex ◽  
Pachera Nathalie ◽  
Rajaei Bahareh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Beta Cells ◽  
Pluripotent Stem Cell ◽  
3D Culture ◽  
Induced Pluripotent Stem Cell ◽  
Culture Systems ◽  
Induced Pluripotent
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