Chemical characterization and antifungal photodynamic effect of methanolic extract and alkaloidal fraction isolated from callus culture of Annona squamosa (Annonaceae)

Introduction: Trypanosoma cruzi is the agent of Chagas’ disease and affects approximately 6-8 million people worldwide. The search for new anti-T. cruzi drugs are relevant because only two drugs exist actually. The objective of this study was to investigate the effect of the extracts from the seeds of Lonchocarpus cultratus on T. cruzi, its cytotoxicity as well as to elucidate its chemical profile. Methodology: The characterization of the extracts was done using 1H-RMN. T. cruzi forms were treated with increasing concentrations of the extracts and after, the percentage of inhibition and IC50 or LC50 were calculated. Murine peritoneal macrophages were treated with different concentrations of the extracts to evaluate the cellular viability. The hemotoxicity was accessed by verifying the levels of hemolysis caused by the extracts on human red blood cells. Results: Chalcones isocordoin and lonchocarpin were detected in the dichloromethane extract, and chalcone lonchocarpin was detected in the hexane extract. The dichloromethane extract showed higher activity against all the forms of T. cruzi compared to the other two extracts, but the hexane showed the best selectivity index. The cytotoxicity observed in murine macrophages was confirmed in human erythrocytes, with dichloromethane extract having the highest toxicity. The methanolic extract showed the lowest anti-T. cruzi activity but was nontoxic to peritoneal murine macrophages and red blood cells. Conclusions: L. cultratus extracts have the potential to be explored for the development of new anti-trypanosomal drugs. This study was the first to demonstrate the action of extracts from the genus Lonchocarpus on infecting forms of T. cruzi.

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Phosphodiesterase-1 Inhibitory Activity of Two Flavonoids Isolated fromPistacia integerrimaJ. L. Stewart Galls

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/506564 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 10

Author(s):

Abdur Rauf ◽

Muhammad Saleem ◽

Ghias Uddin ◽

Bina S. Siddiqui ◽

Haroon Khan ◽

...

Keyword(s):

Inhibitory Activity ◽

In Silico ◽

Molecular Mechanisms ◽

Methanolic Extract ◽

Chemical Constituents ◽

Folk Medicine ◽

Chemical Characterization ◽

Active Constituents ◽

Isolation And Characterization

Pistacia integerrimais one of twenty species among the genusPistacia. Long horn-shaped galls that develop on this plant are harvested and used in Ayurveda and Indian traditional medicine to make “karkatshringi”, a herbal medicine used for the treatment of asthma and different disorders of respiratory tract. However, until now, the molecular mechanisms of action of “karkatshringi” and its chemical characterization are partially known. This study deals with the isolation and characterization of the active constituents from the methanolic extract ofP. integerrimagalls and it was also oriented to evaluatein vitroandin silicotheir potential enzymatic inhibitory activity against phosphodiesterase-1 (PDE1), a well-known enzyme involved in airway smooth muscle activity and airway inflammation. Our results showed that the methanolic extract ofP. integerrimagalls and some of its active constituents [naringenin (1) and 3,5,7,4′-tetrahydroxy-flavanone (2)] are ablein vitroto inhibit PDE1 activity (59.20 ± 4.95%, 75.90 ± 5.90%, and 65.25 ± 5.25%, resp.) and demonstratein silicoan interesting interaction with this enzymatic site. Taken together, our results add new knowledge of chemical constituents responsible for the biological activity ofP. integerrimaand contextually legitimate the use of this plant in folk medicine.

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Chemical characterization and anti-arthritic appraisal of Monotheca buxifolia methanolic extract in Complete Freund’s Adjuvant-induced arthritis in Wistar rats

Inflammopharmacology ◽

10.1007/s10787-020-00783-7 ◽

2021 ◽

Author(s):

Muhammad Furqan Akhtar ◽

Khushdil Khan ◽

Ammara Saleem ◽

Mirza Muhammad Faran Ashraf Baig ◽

Azhar Rasul ◽

...

Keyword(s):

Wistar Rats ◽

Methanolic Extract ◽

Chemical Characterization ◽

Complete Freund’S Adjuvant ◽

Adjuvant Induced Arthritis ◽

Monotheca Buxifolia ◽

Freund’S Adjuvant ◽

Complete Freund's Adjuvant ◽

Freund's Adjuvant

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ANTICANCER EFFECT OF METHANOLIC EXTRACT OF ANNONA SQUAMOSA ON MAMMARY CARCINOMA IN RATS WITH REFERENCE TO GLYCOPROTEIN COMPONENTS, LYSOSOMAL, AND MARKER ENZYMES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i9.17836 ◽

2017 ◽

Vol 10 (9) ◽

pp. 180

Author(s):

Nandhakumar Elumalai ◽

Indumathi Parameswaran ◽

Sachidanandam T Panchanatham

Keyword(s):

Body Weight ◽

Mammary Carcinoma ◽

Lysosomal Enzymes ◽

Methanolic Extract ◽

The Body ◽

Active Principle ◽

Dependent Manner ◽

Marker Enzymes ◽

Annona Squamosa ◽

Anticancer Effect

Objectives: The aim is to evaluate the anticancer potential of Annona squamosa (AS) against 7, 12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinoma in rats.Methods: The tumor was induced in Sprague-Dawley rats by gastric intubation of 25 mg DMBA in 1 ml olive oil. After 3 months of induction period, the methanolic extract of AS at different doses of 100, 150, 200, 250, and 300 mg/kg body weight were administered orally a dose per day to mammary carcinoma-bearing rats for 45 days. The serum and tissue levels of glycoprotein components as well as the activities of marker enzymes and lysosomal enzymes were measured in DMBA-induced mammary carcinoma-bearing rats.Results: Administration of AS resulted in decrease in the levels of marker and lysosomal enzymes and also alterations in the body weight and tumor volume were also restored to near normalcy in a dose-dependent manner. The results of the present study indicate that AS has anticancer effect and it exhibits its potential effect at the dosage of 200 mg/kg body weight in mammary carcinoma-bearing rats.Conclusions: Based on the results obtained, it can be concluded that the methanol extract of AS possesses anticancer properties. Further study is needed to isolate the active principle of this extract responsible for anticancer activity to develop the future pharmaceuticals.

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Chemical Characterization of the Fruit of Annona squamosa L. Occurring in the Amazon

Journal of Food Composition and Analysis ◽

10.1006/jfca.2000.0968 ◽

2001 ◽

Vol 14 (2) ◽

pp. 227-232 ◽

Cited By ~ 32

Author(s):

Eloisa Helena A. Andrade ◽

Maria das Graças B. Zoghbi ◽

José Guilherme S. Maia ◽

Heinz Fabricius ◽

Friedhelm Marx

Keyword(s):

Chemical Characterization ◽

Annona Squamosa

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Evaluation of Wound Healing Activity of Methanolic extract of Annona Squamosa Leaves in Hydrogel delivery system

American Journal of PharmTech Research ◽

10.46624/ajptr.2018.v8.i3.015 ◽

2018 ◽

Vol 8 (3) ◽

pp. 190-198

Author(s):

Himesh Soni ◽

Jitender Malik ◽

Abhay Pratap Yadav ◽

Bhavana Yadav

Keyword(s):

Wound Healing ◽

Delivery System ◽

Methanolic Extract ◽

Annona Squamosa ◽

Wound Healing Activity

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Bio-Guided Fractionation of Retama raetam (Forssk.) Webb & Berthel Polar Extracts

Molecules ◽

10.3390/molecules26195800 ◽

2021 ◽

Vol 26 (19) ◽

pp. 5800

Author(s):

Mariem Saada ◽

Hanen Wasli ◽

Inès Jallali ◽

Rim Kboubi ◽

Karl Girard-Lalancette ◽

...

Keyword(s):

Methanolic Extract ◽

Antibacterial Properties ◽

Chemical Characterization ◽

Polar Compounds ◽

Bioactive Molecules ◽

Prenylated Flavonoids ◽

Retama Raetam ◽

Polar Extracts ◽

Layer Chromatography

The fractionation of the methanolic extract (MeOH-E) of Retama raetam (Forssk.) Webb & Berthel and further analysis by thin layer chromatography resulted in four fractions (F1, F2, F3 and F4) that, in parallel with the MeOH-E, were screened for antioxidant, cytotoxic, antidiabetic and antibacterial properties. In addition, chemical characterization of their bioactive molecules was performed using LC-DAD-ESI/MSn. The results indicated that F3 was the most promising regarding antioxidant and cytotoxicity abilities, possibly due to its richness in flavonoids class, particularly isoflavones. In turn, F1 was characterized by the presence of the most polar compounds from MeOH-E (organic acids and piscidic acid) and showed promising abilities to inhibit α-amylase, while F4, which contained prenylated flavonoids and furanoflavonoids, was the most active against the tested bacteria. The gathered results emphasize the distinct biological potentials of purified fractions of Retama raetam.

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