DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids

2021 ◽  
pp. 475-493
Author(s):  
Akimitsu Okamoto
Keyword(s):  
Nucleic Acids ◽  
Fluorescence Imaging

scholarly journals Improved Synthesis Strategy for Peptide Nucleic Acids (PNA) appropriate for Cell-specific Fluorescence Imaging

2012 ◽  
Vol 9 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Rüdiger Pipkorn ◽  
Manfred Wiessler ◽  
Waldemar Waldeck ◽  
Ute Hennrich ◽  
Kiyoshi Nokihara ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescence Imaging ◽  
Peptide Nucleic Acids ◽  
Specific Fluorescence ◽  
Synthesis Strategy

Abstract 3880: Synthesis of complex oligonucleotide libraries enables flexible and precise high-resolution fluorescence imaging of cellular nucleic acids

2011 ◽  
Author(s):  
Robert A. Ach ◽  
N. Alice Yamada ◽  
Peter Tsang ◽  
Alicia Scheffer-Wong ◽  
Laurakay Bruhn
Keyword(s):  
High Resolution ◽  
Nucleic Acids ◽  
Fluorescence Imaging

scholarly journals Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids

2019 ◽  
Vol 49 (1) ◽  
Author(s):  
Akimitsu Okamoto
Keyword(s):  
Gene Expression ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Fluorescence Imaging ◽  
Cell Function ◽  
Microscopic Analysis ◽  
Next Generation ◽  
Nucleic Acid Probes ◽  
Specific Fluorescence ◽  
Methylated Dna

AbstractFluorescence imaging of nucleic acids is a very important technique necessary to understand gene expression and the resulting changes in cell function. This mini-review focuses on sequence-specific fluorescence imaging of intracellular RNA and methylated DNA using fluorescent nucleic acid probes. A couple of functional fluorescent nucleic acid probes developed by our laboratory are introduced and the examples of their application to fluorescence imaging of intracellular nucleic acids are described.

Vinylnaphthalene-bearing hexaoxazole as a fluorescence turn-on type G-quadruplex ligand

2021 ◽  
Vol 19 (37) ◽  
pp. 8035-8040
Author(s):  
Yue Ma ◽  
Yuki Wakabayashi ◽  
Naruyuki Watatani ◽  
Ryota Saito ◽  
Takatsugu Hirokawa ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Fluorescence Imaging ◽  
Fluorescence Intensity ◽  
Turn On ◽  
G Quadruplex ◽  
Fluorescence Turn On

Cyclic hexaoxazoles bearing vinyl naphthalene moiety is developed as a fluoresence turn-on ligand selectively against G-quadruplex.

Electron Microscopy of Nucleic Acids

1974 ◽  
Vol 32 ◽  
pp. 302-303
Author(s):  
Norman Davidson
Keyword(s):  
Electron Microscopy ◽  
Nucleic Acids ◽  
Basic Protein ◽  
Molecular Biologist ◽  
Topological Properties ◽  
Protein Film ◽  
Polynucleotide Chain

The basic protein film technique for mounting nucleic acids for electron microscopy has proven to be a general and powerful tool for the working molecular biologist in characterizing different nucleic acids. It i s possible to measure molecular lengths of duplex and single-stranded DNAs and RNAs. In particular, it is thus possible to as certain whether or not the nucleic acids extracted from a particular source are or are not homogeneous in length. The topological properties of the polynucleotide chain (linear or circular, relaxed or supercoiled circles, interlocked circles, etc. ) can also be as certained.

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

Nucleic Acid Molecules Prepared by Monolayer Techniques

1972 ◽  
Vol 30 ◽  
pp. 178-179
Author(s):  
Dimitrij Lang
Keyword(s):  
Electron Microscopy ◽  
Standard Deviation ◽  
Nucleic Acid ◽  
Cytochrome C ◽  
Nucleic Acids ◽  
Contour Length ◽  
Sample Standard Deviation ◽  
Molecular Weights ◽  
Length Measurements ◽  
Protein Monolayer

The success of the protein monolayer technique for electron microscopy of individual DNA molecules is based on the prevention of aggregation and orientation of the molecules during drying on specimen grids. DNA adsorbs first to a surface-denatured, insoluble cytochrome c monolayer which is then transferred to grids, without major distortion, by touching. Fig. 1 shows three basic procedures which, modified or not, permit the study of various important properties of nucleic acids, either in concert with other methods or exclusively:1) Molecular weights relative to DNA standards as well as number distributions of molecular weights can be obtained from contour length measurements with a sample standard deviation between 1 and 4%.

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

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