Comparisons between doubled haploid lines produced by anther culture, the Hordeum bulbosum-method and lines produced by single seed descent in barley crosses

Euphytica ◽  
1993 ◽  
Vol 66 (1-2) ◽  
pp. 135-144 ◽  
Author(s):  
�. Bj�rnstad ◽  
H. Skinnes ◽  
K. Thoresen
1989 ◽  
Vol 103 (2) ◽  
pp. 101-109 ◽  
Author(s):  
P. S. Baenziger ◽  
D. M. Wesenberg ◽  
V. M. Smail ◽  
W. L. Alexander ◽  
G. W. Schaeffer

2017 ◽  
Vol 1 (1) ◽  
pp. 99-100
Author(s):  
Oksana Ivanovna Zaitseva

Abstract The technology for in vitro anther culturing was optimized in hexaploid triticale using combination of external factors that allowed to obtain more than 100 doubled haploid lines. Investigation of genetic variation among anther culture derived doubled haploids of triticale showed the occurrence of heterozygous plants.


2018 ◽  
Vol 46 (2) ◽  
pp. 119 ◽  
Author(s):  
Muhammad Fuad Anshori ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Sintho Wahyuning Ardie ◽  
Willy Bayuardi Suwarno ◽  
...  

<p>ABSTRACT<br /><br />Doubled haploid rice formation through anther culture becomes one of the alternatives in the plant breeding. The lines need to be characterized, clustered, and selected. This study aimed at determining heritability, characters, and cluster of doubled haploid lines obtained from anther culture. This experiment was conducted at IPB Rice Field Experiment Station, Darmaga, Bogor from October 2016 until January 2017. The experiment was managed according to randomized complete block design (RCBD) with 3 replications and genotype as the single factor treatment. Genotypes used were 60 genotypes consisting of 56 doubled haploid lines and 4 control varieties. The controls were Ciherang, Inpara 5, Inpari 29, and Inpari 34 Salin Agritan. The results showed that all characters have significant effect and high heritability (above 60%), so they could be used as a selection character. The tested genotypes could be grouped into three main groups. The first group consisted of 11 genotypes, the second group consisted of 44 genotypes, and the third group consisted of 5 genotypes. The main group determinant characters were productive tillers, percentage of filled grain number, plant height, panicle length, and weight of 1,000 grains. The number of filled grain and productive tillers can be used as selection characters together with yield potential.<br /><br />Keywords: filled grain, heatmap, panicle length, productive tillers, yield potential<br /><br /></p>


2020 ◽  
Vol 56 (No. 4) ◽  
pp. 150-158
Author(s):  
Osama Zuhair Kanbar ◽  
Csaba Lantos ◽  
Paul Karumba Chege ◽  
Erzsébet Kiss ◽  
János Pauk

We investigated the anther culture (AC) efficiency of thirteen F<sub>4</sub> combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.


2016 ◽  
Vol 8 (5) ◽  
pp. 177 ◽  
Author(s):  
M. Fazaa ◽  
A. EL Sabagh ◽  
G. Anis ◽  
I. EL-Rewainy ◽  
C. Barutcular ◽  
...  

<p>Anther culture technique offers great opportunities to accelerate breeding progress. The current study aimed to develop some good doubled haploid lines (DH) through anther culture technique and to evaluate them along with their five parents including two check varieties for yield and yield related traits. Analysis of variance revealed that varietal differences were signified and wide variability present among the genotypes with respect to all the characteristics studied. Correlation analysis revealed that grain yield was highly positive correlated with panicle length. The information on the inter association among the yield attributing characters showed the nature and extent of relationship with each other. Consequently, this will help in the improvement of different characters along with yield in breeding programmes.</p>


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