scholarly journals Heritabilitas, Karakterisasi, dan Analisis Clustergram Galur-galur Padi Dihaploid Hasil Kultur Antera

2018 ◽  
Vol 46 (2) ◽  
pp. 119 ◽  
Author(s):  
Muhammad Fuad Anshori ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Sintho Wahyuning Ardie ◽  
Willy Bayuardi Suwarno ◽  
...  

<p>ABSTRACT<br /><br />Doubled haploid rice formation through anther culture becomes one of the alternatives in the plant breeding. The lines need to be characterized, clustered, and selected. This study aimed at determining heritability, characters, and cluster of doubled haploid lines obtained from anther culture. This experiment was conducted at IPB Rice Field Experiment Station, Darmaga, Bogor from October 2016 until January 2017. The experiment was managed according to randomized complete block design (RCBD) with 3 replications and genotype as the single factor treatment. Genotypes used were 60 genotypes consisting of 56 doubled haploid lines and 4 control varieties. The controls were Ciherang, Inpara 5, Inpari 29, and Inpari 34 Salin Agritan. The results showed that all characters have significant effect and high heritability (above 60%), so they could be used as a selection character. The tested genotypes could be grouped into three main groups. The first group consisted of 11 genotypes, the second group consisted of 44 genotypes, and the third group consisted of 5 genotypes. The main group determinant characters were productive tillers, percentage of filled grain number, plant height, panicle length, and weight of 1,000 grains. The number of filled grain and productive tillers can be used as selection characters together with yield potential.<br /><br />Keywords: filled grain, heatmap, panicle length, productive tillers, yield potential<br /><br /></p>

Author(s):  
Mohammad Syafii ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Willy Bayuardi Suwarno

<p><em></em><em>Anther culture is useful tool in rice breeding. The technique shortens the time to obtain full homozygous plant in just one generation. Information related to genetic variability among the regenerants is important for further analysis including selection activities. The objective of this study was to analyze agronomic character variability and yield potential among doubled-haploid lines (DH0) derived from anther culture. This study was carried out in Center for Agricultural Biotechnology and Genetic Resources Research and Development (BB Biogen) from October 2016 to February 2017. The experiment was arranged in randomized complete block design (RCBD) with three replications. Forty eight doubled haploid lines derived from anther culture and three check varieties were evaluated. The results showed high genetic variability as well as broad sense heritability (more than 90%) for all variables tested. Several lines showed superior agronomic characters compared to check (Ciherang, Inpari 18, Inpago 10). Anther culture could generate high genetic variability for further selection.<br /><br />Keywords: anther culture, broad sense heritability, haploid technology</em></p>


2016 ◽  
Vol 17 (1) ◽  
pp. 19
Author(s):  
Rini Hermanasari ◽  
NFN Supartopo ◽  
B. Kustianto

<p>Phenotypic Performance of Yield and Yield Component of Rice Promising Lines at Tidal Swamp Area in Karang Agung, South Sumatra. An experiment was conducted in Karang Agung, in April until September 2007. The experiment aimed at evaluating phenotypic performance of yield and yield components of promising lines in tidal swamp rice field. The experiment was arranged in a Randomized Complete Block Design with 16 treatments and four replications. The promising lines tested were B9858-KA-55, B9833C-KA-14, B9852E-KA-66, B5524G-SM-61-2-1, B7003D-MR-24-3-1, KAL9414F-MR-2-KN-0, KAL9418F-MR-2-KN-0, B10214FTB- 7-2-3, IR70213-9-CPA-12-UBN-2-1-3-1, IR70215-2- CPA-2-1-B-1-2. The check varieties used were IR42, Batanghari and Lembu Sawah. The results of this experiment showed that IR61242-3B-B-2 had better performance among varieties and promising lines tested, as well as giving the highest yield potential. Meanwhile, the line B10214F-TB-7-2- 3 had better performance of number of filled grain, and 1.000 grain weight. It also showed higher yield potential compared to IR42 and Batanghari. The line KAL9418F-MR-2-KN-0 had better performance of plant height, 1.000 weight grain and harvest age, but showed lower yield potential compared to those other check varieties.</p><p> </p><p><strong>Abstrak</strong></p><p>Percobaan dilaksanakan di KP Karang Agung, Sumatera Selatan pada bulan April sampai September 2007. Penelitian bertujuan untuk mendapatkan informasi tentang penampilan fenotipik karakter hasil dan komponen hasil dari galur-galur harapan padi rawa di lahan pasang surut Karang Agung. Percobaan ditata dalam rancangan acak kelompok dengan 16 perlakuan dan empat ulangan. Galur-galur yang diuji di antaranya B9858-KA-55, B9833C-KA-14, B9852E-KA-66, B5524GSM- 61-2-1, B7003D-MR-24-3-1, KAL9414F-MR-2-KN-0, KAL9418F-MR-2-KN-0, B10214F-TB-7-2-3, IR70213-9- CPA-12-UBN-2-1-3-1, dan IR70215-2-CPA-2-1-B-1-2. Varietas pembanding digunakan IR42, Batanghari, dan Lembu Sawah. Hasil penelitian menunjukkan bahwa galur IR61242- 3B-B-2 memiliki penampilan lebih baik dari varietas pembanding untuk karakter tinggi tanaman, jumlah gabah isi, bobot 1.000 butir gabah, dan potensi hasil. Galur B10214F-TB-7-2-3 memberikan penampilan lebih baik untuk karakter jumlah gabah isi, bobot 1.000 butir, dan potensi hasil lebih tinggi daripada pembanding IR42 dan Batanghari. Galur KAL9418F-MR- 2-KN-0 memiliki penampilan lebih baik untuk karakter bobot 1.000 butir, tinggi tanaman, dan umur panen, tetapi potensi hasilnya lebih rendah dari ketiga varietas pembanding.</p>


2016 ◽  
Vol 8 (5) ◽  
pp. 177 ◽  
Author(s):  
M. Fazaa ◽  
A. EL Sabagh ◽  
G. Anis ◽  
I. EL-Rewainy ◽  
C. Barutcular ◽  
...  

<p>Anther culture technique offers great opportunities to accelerate breeding progress. The current study aimed to develop some good doubled haploid lines (DH) through anther culture technique and to evaluate them along with their five parents including two check varieties for yield and yield related traits. Analysis of variance revealed that varietal differences were signified and wide variability present among the genotypes with respect to all the characteristics studied. Correlation analysis revealed that grain yield was highly positive correlated with panicle length. The information on the inter association among the yield attributing characters showed the nature and extent of relationship with each other. Consequently, this will help in the improvement of different characters along with yield in breeding programmes.</p>


Agric ◽  
2021 ◽  
Vol 33 (1) ◽  
pp. 57-66
Author(s):  
Kiki Kusyaeri Hamdani ◽  
Yati Haryati

New superior varieties (VUB) are a reliable technological innovation to increase rice productivity. This study aims to determine the yield potential of some lowland rice VUB. The assessment was carried out on land owned by a member of the Sumber Rejeki Farmer Group, Cintaratu Village, Lakbok District, Ciamis Regency at Dry Season II in June-September 2020. The study used a randomized complete block design (RCBD) with six varieties of treatment and was repeated ten times. The varieties tested were Inpari 32, Inpari 42, Padjadjaran, Cakrabuana, Inpari IR Nutrizinc, and Siliwangi varieties. The variables observed included the growth component, yield component, and yield component. Data were analyzed using the F test followedby the Duncan Multiple Range Test at the Q=5% level. In addition, a correlation test was conducted between the growth components, yield components, and yields. The results of the study indicated that the new superior rice varieties studied had different performance in growth, number of tillers, yield, and yield components. Inpari 42 variety produced the highest productivity, namely 6.88 ton ha-1 which was supported by the number of grains per panicle, percentage of filled grains per panicle, and percentage of empty grain per panicle which were better than other varieties. Plant height and number of grains per panicle were positively correlated with yield.


2012 ◽  
Vol 12 (2) ◽  
pp. 93 ◽  
Author(s):  
Budi Winarto ◽  
Nurhayati Ansori Mattjik ◽  
Agus Purwito ◽  
Budi Marwoto

Improvement of selected induction culture media on callus induction in anther culture of anthurium and a histologicalstudy on its callus formation were studied at the tissue culture laboratory of the Indonesian Ornamental CropsResearch Institute from February to October 2008. The objectives of the study were to optimize selected media forcallus formation, reveal cell origin of callus derived from anther culture and shoot formation process. Selectedmedia improved in the study were 1) MMS-TBN containing 0,5 mg/l TDZ, 1,0 mg/l BAP and 0,01 mg/l NAA (Winartomedium, WM) and 2) MMS III supplemented with 1,5 mg/l TDZ, 0,75 mg/l BAP and 0,02 mg/l NAA (Winarto andRachmawati medium, WRM). Improvement treatments were carried out by omission and application of 2,4-D in 0.5mg/l and reduction of medium strength of full, half, quarter, one eighth, one sixteenth, and zero strength. Afactorial experiment was arranged using a randomized complete block design with four replications. Results ofthis study indicated that the highest callus induction was clearly established in WRM. The medium stimulatedpotential growth of anther (PGA) up to 81% with 49% of percentage of anther regeneration (PAR) and 2.7 number ofcallus formed per replication (NCF). Significant improvement in callus formation was also recorded by reduction ofmedium strength of WRM to one eighth compared to others. The reduction induced PGA up to 58% with 29% of PARand 1.8 NCF. From histological studies it was well recognized that regenerated callus on half anthers cultured wasoriginated from middle layer cells of anther wall. The morphogenic response of anther wall cells caused primarilyon no androgenesis effect in microspore cells.


2021 ◽  
Vol 23 (1) ◽  
pp. 6
Author(s):  
Fajriyatus Shoidah ◽  
Adnan Adnan

<p>New wetlands field generally has low productivity, so a technology is needed for improve productivity. New Superior Variety (NSV) is a technology that is considered easy to be adopted by farmers, so knowing a NSV that is able to adapt to the characteristics and conditions of new wetlands field is a must. The study of adaptive NSV on new wetlands field in Boven Digoel Regency is still limited so that the NSV recommendation for farmers is also still limited. The purpose of this study was to examine the growth and production of new superior varieties on the new wetlands fields in Boven Digoel Regency. The study was conducted on new wetlands in Asiki Village, Jair District during April-August 2019. Randomized Complete Block Design was employed with 3 replications using Inpari 36, Inpari 37, Inpago Rindang 1, Inpago Rindang 2 dan Inpara 8. The results showed different varieties perform different agronomy characters observed (plant height, number of tillers, panicle length, number of grains per panicle, filled grain, per panicle, empty grain per panicle and 1000 grain weight). Inpari 37 produced 5.3 ton/ha Dried Unhulled Rice, the highest production compared to other varieties, so that it could potentially be developed in Boven Digoel’s new wetlands field. Productivity may still be increased by improving soil fertility conditions.</p>


1980 ◽  
Vol 60 (1) ◽  
pp. 79-85 ◽  
Author(s):  
PIERRE TURCOTTE ◽  
C. A. ST-PIERRE ◽  
KEH MING HO

Pedigree and doubled haploid lines from seven crosses of barley (Hordeum vulgare L.) were compared over 2 years. The lines were tested, in a randomized complete block design, in row plots at Ste-Foy in 1977 and in hill plots at Brawley, California in 1978. There are significant differences between the two methods of pure line production for grain yield, 1000-kernel weight, plant height, resistance to lodging and date of maturity. Furthermore, these significant differences between doubled haploid and pedigree lines seem to be tied to wide crosses. After showing that doubled haploids are superior for resistance to lodging, we conclude that the use of doubled haploid lines must be seriously considered in a barley breeding program on the basis of improved selection efficiency and the short time required to get homozygous lines.


1989 ◽  
Vol 103 (2) ◽  
pp. 101-109 ◽  
Author(s):  
P. S. Baenziger ◽  
D. M. Wesenberg ◽  
V. M. Smail ◽  
W. L. Alexander ◽  
G. W. Schaeffer

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Samuel Oppong Abebrese ◽  
Alex Yeboah ◽  
Wilson Dogbe ◽  
Paul Kofi Ayirebi Dartey ◽  
Richard Akromah ◽  
...  

Hybrid rice technology is one of the promising, sustainable, and proven technologies for increasing rice production and productivity with a yield advantage of 15–30% over modern inbred varieties. The potential of hybrid rice has so far not been exploited in Ghana. This study was undertaken to evaluate the yield potential, reaction to diseases, and physical grain attributes of some introduced hybrids. The trials were laid out in a randomized complete block design (RCBD) with three replicates across three locations. Data were taken on grain yield, yield components, reaction to diseases, and grain physical characteristics. Four promising hybrids (SWARNA 2, ARGH 1501, ARGH 1502, and ARGH 1503) with a mean yield advantage of 15–20.8% over the best inbred check “AgraRice” were identified. With few exceptions, the hybrids were broadly adapted and had adequate resistance to blast and bacterial leaf blight. Most of the test hybrids had long slender grains which make them acceptable to the Ghanaian market but lacked aroma.


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