Variation of characters in near-isogenic lines of wheat with added genes for leaf rust resistance

Euphytica ◽  
1990 ◽  
Vol 45 (1) ◽  
pp. 87-92
Author(s):  
P. M. Fried ◽  
H. Winzeler



Crop Science ◽  
2008 ◽  
Vol 48 (4) ◽  
pp. 1441-1451 ◽  
Author(s):  
Juan Carlos Brevis ◽  
Oswaldo Chicaiza ◽  
Imtiaz A. Khan ◽  
Lee Jackson ◽  
Craig F. Morris ◽  
...  


1995 ◽  
Vol 73 (7) ◽  
pp. 1081-1088 ◽  
Author(s):  
J. A. Kolmer

A heterogeneous population of Puccinia recondita f.sp. tritici developed from randomly mated pycnial infections on Thalictrum speciosissimum was selected for 12 generations on three multilines composed of Thatcher wheat lines near-isogenic for leaf rust resistance genes. Multiline 1 was composed of 20% each of lines near-isogenic for resistance genes Lr2a, Lr3ka, Lr11, Lr17, and Lr24. Multiline 2 was composed of equal proportions each of Thatcher and the five near-isogenic lines in multiline 1. Multiline 3 was composed of 50% Thatcher and 10% each of the five near-isogenic lines. Diversity of virulence phenotypes as measured by the Shannon index declined most in the population selected on multiline 1. Phenotypes of P. r. tritici with virulence to three of the five resistance genes in the multilines were the predominant isolates in all three populations after 12 generations of selection. Frequency of phenotypes with virulence to resistance genes Lr2a and Lr11 significantly increased in all three populations. Virulence to Lr11 significantly increased in the multiline 3 population, and virulence to Lr24 increased in populations from multilines 2 and 3. Frequency of phenotypes with virulence to Lr3ka did not significantly change in any of the populations. Virulence phenotypes with an intermediate number of virulences appeared to have fitness advantage relative to other isolates on the three host multilines. These results indicate that use of wheat multilines may not necessarily stabilize selection for virulence complexity in P. r. tritici populations. Key words: mixtures, Triticum aestivum, wheat leaf rust.







Author(s):  
Subodh Kumar ◽  
Subhash C. Bhardwaj ◽  
Om P. Gangwar ◽  
Akanksha Sharma ◽  
Naeela Qureshi ◽  
...  


Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 655-664 ◽  
Author(s):  
Li Huang ◽  
Steven A Brooks ◽  
Wanlong Li ◽  
John P Fellers ◽  
Harold N Trick ◽  
...  

Abstract We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.



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