Quantitative analysis of revascularisation and new bone formation in experimental bone grafts

1993 ◽  
Vol 17 (2) ◽  
Author(s):  
H. Yano ◽  
S. Masumi ◽  
T. Fukunaga ◽  
S. Ikebe
Keyword(s):  
Quantitative Analysis ◽  
Bone Formation ◽  
Bone Grafts ◽  
New Bone Formation

Quantitative Analysis of New Bone Formation in Various Bone Grafts by In - Vivo Tetracycline Labeling

1985 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Sang Cheol Seong ◽  
Young Min Kim ◽  
Han Koo Lee ◽  
In Ho Choi ◽  
Moon Sang Chung ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Bone Formation ◽  
Bone Grafts ◽  
New Bone Formation ◽  
Tetracycline Labeling

Evaluation of Gamma Irradiated Human Bone in Nude Rats

2005 ◽  
Vol 288-289 ◽  
pp. 269-272
Author(s):  
Y. Yu ◽  
Jin Biao Chen ◽  
J.L. Yang ◽  
D.A.F. Morgan ◽  
W.R. Walsh
Keyword(s):  
Bone Formation ◽  
Gamma Irradiation ◽  
Soft Tissues ◽  
Bone Grafts ◽  
Human Bone ◽  
New Bone Formation ◽  
Nude Rats ◽  
Defect Area ◽  
Post Operation ◽  
Gamma Irradiated

Deep-frozen morselized human bone grafts showed osteoconductivity and osteoinductivity when implanted into tibial window defects of nude rats. The osteoconductivity was assessed by measuring the total area of newly formed bone bridged by the implanted bone grafts in the entire defect area. The osteoinductivity was evidenced by the presence of active osteoblast-like cells and new bone formation around the implanted bone grafts, which were surrounded by soft tissues distant from the host cortex. Gamma irradiation at the doses of 15 or 25 kGray reduced the osteoconductivity (ANOVA and LSD tests, p<0.05) at 3 weeks post operation. The 25 kGray group had a significantly lower level of new bone formation compared with the 0 and 15 kGray groups. The evidences of osteoinductivity were only noted in the 0 and 15 kGray groups. Our data indicate that 25 kGray gamma irradiation reduces the osteoconductive and osteoinductive properties of the morselized human bone graft.

Retracted: New bone formation in bone defects after melatonin and porcine bone grafts: experimental study in rabbits

2014 ◽  
Vol 26 (4) ◽  
pp. 399-406 ◽  
Author(s):  
José-Luis Calvo-Guirado ◽  
Gerardo Gómez-Moreno ◽  
José-Eduardo Maté-Sánchez ◽  
Laura López-Marí ◽  
Rafael Delgado-Ruiz ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Formation ◽  
Bone Grafts ◽  
Bone Defects ◽  
New Bone Formation

New Bone Formation in the Maxillary Sinus Without Bone Grafts

2008 ◽  
Vol 17 (3) ◽  
pp. 321-331 ◽  
Author(s):  
Dong-Seok Sohn ◽  
Ji-soo Lee ◽  
Mi-ra Ahn ◽  
Hong-In Shin
Keyword(s):  
Bone Formation ◽  
Maxillary Sinus ◽  
Bone Grafts ◽  
New Bone Formation

Effect of recombinant human bone morphogenic protein 9 (rhBMP9) loaded onto bone grafts versus barrier membranes on new bone formation in a rabbit calvarial defect model

2017 ◽  
Vol 105 (10) ◽  
pp. 2655-2661 ◽  
Author(s):  
Masako Fujioka-Kobayashi ◽  
Eizaburo Kobayashi ◽  
Benoit Schaller ◽  
Matthias Mottini ◽  
Richard J. Miron ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Grafts ◽  
Bone Morphogenic Protein ◽  
Human Bone ◽  
Calvarial Defect ◽  
New Bone Formation ◽  
Defect Model ◽  
Barrier Membranes ◽  
Recombinant Human Bone

Revascularization and new bone formation in heat-treated bone grafts

1999 ◽  
Vol 119 (1-2) ◽  
pp. 57-61 ◽  
Author(s):  
K. Shimizu ◽  
S. Masumi ◽  
H. Yano ◽  
T. Fukunaga ◽  
S. Ikebe ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Grafts ◽  
New Bone Formation ◽  
Heat Treated

scholarly journals Settable Polymeric Autograft Extenders in a Rabbit Radius Model of Bone Formation

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3960
Author(s):  
Lauren A. Boller ◽  
Madison A.P. McGough ◽  
Stefanie M. Shiels ◽  
Craig L. Duvall ◽  
Joseph C. Wenke ◽  
...  
Keyword(s):  
Bone Formation ◽  
Gold Standard ◽  
Bone Growth ◽  
Bone Grafts ◽  
Polymer Composition ◽  
Cellular Infiltration ◽  
New Bone Formation ◽  
Defect Model ◽  
Patient Morbidity

Autograft (AG) is the gold standard for bone grafts, but limited quantities and patient morbidity are associated with its use. AG extenders have been proposed to minimize the volume of AG while maintaining the osteoinductive properties of the implant. In this study, poly(ester urethane) (PEUR) and poly(thioketal urethane) (PTKUR) AG extenders were implanted in a 20-mm rabbit radius defect model to evaluate new bone formation and graft remodeling. Outcomes including µCT and histomorphometry were measured at 12 weeks and compared to an AG (no polymer) control. AG control examples exhibited new bone formation, but inconsistent healing was observed. The implanted AG control was resorbed by 12 weeks, while AG extenders maintained implanted AG throughout the study. Bone growth from the defect interfaces was observed in both AG extenders, but residual polymer inhibited cellular infiltration and subsequent bone formation within the center of the implant. PEUR-AG extenders degraded more rapidly than PTKUR-AG extenders. These observations demonstrated that AG extenders supported new bone formation and that polymer composition did not have an effect on overall bone formation. Furthermore, the results indicated that early cellular infiltration is necessary for harnessing the osteoinductive capabilities of AG.

scholarly journals Estimation of the Effect of Accelerating New Bone Formation of High and Low Molecular Weight Hyaluronic Acid Hybrid: An Animal Study

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1708
Author(s):  
Po-Jan Kuo ◽  
Hsiu-Ju Yen ◽  
Chi-Yu Lin ◽  
Hsuan-Yu Lai ◽  
Chun-Hung Chen ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Bone Formation ◽  
Bone Graft ◽  
Animal Study ◽  
Bone Grafts ◽  
Bone Defects ◽  
Low Molecular Weight ◽  
Potential Candidate ◽  
New Bone Formation

Osteoconduction is an important consideration for fabricating bio-active materials for bone regeneration. For years, hydroxyapatite and β-calcium triphosphate (β-TCP) have been used to develop bone grafts for treating bone defects. However, this material can be difficult to handle due to filling material sagging. High molecular weight hyaluronic acid (H-HA) can be used as a carrier to address this problem and improve operability. However, the effect of H-HA on bone formation is still controversial. In this study, low molecular weight hyaluronic acid (L-HA) was fabricated using gamma-ray irradiation. The viscoelastic properties and chemical structure of the fabricated hybrids were evaluated by a rheological analysis nuclear magnetic resonance (NMR) spectrum. The L-MH was mixed with H-HA to produce H-HA/L-HA hybrids at ratios of 80:20, 50:50 and 20:80 (w/w). These HA hybrids were then combined with hydroxyapatite and β-TCP to create a novel bone graft composite. For animal study, artificial bone defects were prepared in rabbit femurs. After 12 weeks of healing, the rabbits were scarified, and the healing statuses were observed and evaluated through micro-computer tomography (CT) and tissue histological images. Our viscoelastic analysis showed that an HA hybrid consisting 20% H-HA is sufficient to maintain elasticity; however, the addition of L-HA dramatically decreases the dynamic viscosity of the HA hybrid. Micro-CT images showed that the new bone formations in the rabbit femur defect model treated with 50% and 80% L-HA were 1.47 (p < 0.05) and 2.26 (p < 0.01) times higher than samples filled with HA free bone graft. In addition, a similar tendency was observed in the results of HE staining. These results lead us to suggest that the material with an H-HA/L-HA ratio of 50:50 exhibited acceptable viscosity and significant new bone formation. Thus, it is reasonable to suggest that it may be a potential candidate to serve as a supporting system for improving the operability of granular bone grafts and enhancing new bone formations.

scholarly journals New Bone Formation around Implants Inserted on Autologous and Xenografts Irradiated or not with IR Laser Light: A Histomorphometric Study in Rabbits

2013 ◽  
Vol 24 (3) ◽  
pp. 218-223 ◽  
Author(s):  
Luiz Guilherme P. Soares ◽  
Edival Barreto de Magalhaes Junior ◽  
Cesar Augusto B. Magalhaes ◽  
Cimara Fortes Ferreira ◽  
Aparecida Maria C. Marques ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Grafts ◽  
Systemic Effect ◽  
Low Level Laser Therapy ◽  
New Bone Formation ◽  
Contact Mode ◽  
Bone Implant ◽  
Histomorphometric Study ◽  
Significant Difference ◽  
Autologous Bone Grafts

Use of biomaterials and light on bone grafts has been widely reported. This work assessed the influence of low-level laser therapy (LLLT) on bone volume (BV) and bone implant contact (BIC) interface around implants inserted in blocks of bovine or autologous bone grafts (autografts), irradiated or not, in rabbit femurs. Twenty-four adult rabbits were divided in 8 groups: AG: autograft; XG: xenograft; AG/L: autograft + laser; XG/L: xenograft + laser; AG/I: autograft + titanium (Ti) implant; XG/I: xenograft + Ti implant; AG/I/L: autograft + Ti implant + laser; and XG/I/L: xenograft + Ti implant + laser. The animals received the Ti implant after incorporation of the grafts. The laser parameters in the groups AG/L and XG/L were λ=780 nm, 70 mW, CW, 21.5 J/cm 2 , while in the groups AG/I/L and XG/I/L the following parameters were used: λ=780 nm, 70 mW, 0.5 cm 2 (spot), 4 J/cm 2 per point (4), 16 J/cm 2 per session, 48 h interval × 12 sessions, CW, contact mode. LLLT was repeated every other day during 2 weeks. To avoid systemic effect, only one limb of each rabbit was double grafted. All animals were sacrificed 9 weeks after implantation. Specimens were routinely stained and histomorphometry carried out. Comparison of non-irradiated and irradiated grafts (AG/L versus AG and XG/L versus XG) showed that irradiation increased significantly BV on both grafts (p=0.05, p=0.001). Comparison between irradiated and non-irradiated grafts (AG/I/L versus AG/I and XG/I/L versus XG/I) showed a significant (p=0.02) increase of the BIC in autografts. The same was seen when xenografts were used, without significant difference. The results of this investigation suggest that the use of LLLT is effective for enhancing new bone formation with consequent increase of bone-implant interface in both autologous grafts and xenografts.

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