Electromicrobial regeneration of pyridine nucleotides and other preparative redox transformations with Clostridium thermoaceticum

1995 ◽  
Vol 42 (6) ◽  
pp. 916-922 ◽  
Author(s):  
M. Schulz ◽  
H. Leichmann ◽  
H. Günther ◽  
H. Simon
Keyword(s):  
Pyridine Nucleotides ◽  
Clostridium Thermoaceticum ◽  
Redox Transformations

scholarly journals SPECTROPHOTOMETRIC ESTIMATION OF PYRIDINE NUCLEOTIDES IN ANIMAL TISSUES

1950 ◽  
Vol 185 (2) ◽  
pp. 741-747 ◽  
Author(s):  
Philip. Feigelson ◽  
J.N. Williams ◽  
C.A. Elvehjem
Keyword(s):  
Pyridine Nucleotides ◽  
Animal Tissues

scholarly journals Creating enzymes and self-sufficient cells for biosynthesis of the non-natural cofactor nicotinamide cytosine dinucleotide

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xueying Wang ◽  
Yanbin Feng ◽  
Xiaojia Guo ◽  
Qian Wang ◽  
Siyang Ning ◽  
...  
Keyword(s):  
Biological Sciences ◽  
Escherichia Coli ◽  
Nicotinamide Adenine Dinucleotide ◽  
Chemical Biology ◽  
Reduced Form ◽  
Redox Transformations ◽  
Nicotinamide Mononucleotide ◽  
Self Sufficiency ◽  
Binding Pockets ◽  
Pathway Regulation

AbstractNicotinamide adenine dinucleotide (NAD) and its reduced form are indispensable cofactors in life. Diverse NAD mimics have been developed for applications in chemical and biological sciences. Nicotinamide cytosine dinucleotide (NCD) has emerged as a non-natural cofactor to mediate redox transformations, while cells are fed with chemically synthesized NCD. Here, we create NCD synthetase (NcdS) by reprograming the substrate binding pockets of nicotinic acid mononucleotide (NaMN) adenylyltransferase to favor cytidine triphosphate and nicotinamide mononucleotide over their regular substrates ATP and NaMN, respectively. Overexpression of NcdS alone in the model host Escherichia coli facilitated intracellular production of NCD, and higher NCD levels up to 5.0 mM were achieved upon further pathway regulation. Finally, the non-natural cofactor self-sufficiency was confirmed by mediating an NCD-linked metabolic circuit to convert L-malate into D-lactate. NcdS together with NCD-linked enzymes offer unique tools and opportunities for intriguing studies in chemical biology and synthetic biology.

scholarly journals Nicotinamide Effectively Suppresses Fusarium Head Blight in Wheat Plants

2021 ◽  
Vol 22 (6) ◽  
pp. 2968
Author(s):  
Yasir Sidiq ◽  
Masataka Nakano ◽  
Yumi Mori ◽  
Takashi Yaeno ◽  
Makoto Kimura ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fungal Pathogen ◽  
Antifungal Compounds ◽  
Pyridine Nucleotides ◽  
Fungal Metabolites ◽  
Dna Hypomethylation ◽  
Metabolome Analysis ◽  
Head Blight ◽  
Ergosterol Peroxide ◽  
Nicotinamide Mononucleotide

Pyridine nucleotides such as a nicotinamide adenine dinucleotide (NAD) are known as plant defense activators. We previously reported that nicotinamide mononucleotide (NMN) enhanced disease resistance against fungal pathogen Fusarium graminearum in barley and Arabidopsis. In this study, we reveal that the pretreatment of nicotinamide (NIM), which does not contain nucleotides, effectively suppresses disease development of Fusarium Head Blight (FHB) in wheat plants. Correspondingly, deoxynivalenol (DON) mycotoxin accumulation was also significantly decreased by NIM pretreatment. A metabolome analysis showed that several antioxidant and antifungal compounds such as trigonelline were significantly accumulated in the NIM-pretreated spikes after inoculation of F. graminearum. In addition, some metabolites involved in the DNA hypomethylation were accumulated in the NIM-pretreated spikes. On the other hand, fungal metabolites DON and ergosterol peroxide were significantly reduced by the NIM pretreatment. Since NIM is relative stable and inexpensive compared with NMN and NAD, it may be more useful for the control of symptoms of FHB and DON accumulation in wheat and other crops.

scholarly journals Pyridine Nucleotides in the Thyroid

1963 ◽  
Vol 238 (10) ◽  
pp. 3366-3368 ◽  
Author(s):  
Ira Pastan ◽  
Phyllis Johnson ◽  
Esther Kendig ◽  
James B. Field
Keyword(s):  
Pyridine Nucleotides

scholarly journals OXIDATION OF PYRIDINE NUCLEOTIDES COUPLED TO PHOSPHORYLATION

1957 ◽  
Vol 229 (2) ◽  
pp. 771-779
Author(s):  
Sudha Joshi ◽  
R.W. Newburgh ◽  
Vernon H. Cheldelin
Keyword(s):  
Pyridine Nucleotides
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