A blue-light photoreceptor mediates the fluence-rate-dependent expression of genes encoding the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase in light-grown Phaseolus vulgaris primary leaves

Planta ◽  
1993 ◽  
Vol 192 (1) ◽  
Author(s):  
TimI. Sawbridge ◽  
Enrique L�pez-Juez ◽  
MarcR. Knight ◽  
GarethI. Jenkins
Keyword(s):  
Phaseolus Vulgaris ◽  
Blue Light ◽  
Small Subunit ◽  
Fluence Rate ◽  
Bisphosphate Carboxylase ◽  
Rate Dependent ◽  
Blue Light Photoreceptor ◽  
Expression Of Genes ◽  
Genes Encoding

Changes in protein and gene expression during induction of the CO2-concentrating mechanism in wild-type and mutant Chlamydomonas

1991 ◽  
Vol 69 (5) ◽  
pp. 1008-1016 ◽  
Author(s):  
Martin H. Spalding ◽  
Thomas L. Winder ◽  
James C. Anderson ◽  
Anne M. Geraghty ◽  
Laura F. Marek
Keyword(s):  
Transcript Abundance ◽  
Small Subunit ◽  
Wild Type ◽  
Bisphosphate Carboxylase ◽  
Phosphoglycolate Phosphatase ◽  
Concentrating Mechanism ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
In The Wild ◽  
Inorganic Carbon Transport

Several changes occur in wild-type Chlamydomonas reinhardtii upon exposure to limiting CO2, including induction of several polypeptides. Polypeptide induction was previously shown to correlate with appearance of the active CO2-concentrating mechanism (CCM) of this alga. In this paper induction of polypeptides by limiting CO2 was investigated in mutants with lesions in the CCM. Mutants with lesions in the ca-1 and pmp-1 loci exhibited alterations in polypeptide induction, but it was concluded that the alterations probably do not represent their primary genetic lesions. Other changes that occur in this alga in response to limiting CO2 were also investigated. Based on a lack of significant change in the transcript abundance of ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunit genes in the wild type, it was concluded that the previously reported transient decline in synthesis of both subunits is controlled at the translational level. A transient increase in the activity of the photorespiratory enzyme phosphoglycolate phosphatase was observed in the wild type but not in a mutant, cia-5, that lacks induction of the CCM. In addition, changes in expression of genes encoding periplasmic carbonic anhydrase, a 36-kDa membrane-associated protein and a chlorophyll-binding protein occurred in the wild type but not in cia-5 in response to limiting CO2. The absence of these changes in cia-5 was attributed to a lack of either the signal itself or transduction of the signal responsible for adaptation to limiting CO2, which led to speculation that a larger range of responses are regulated by the same signal than was previously recognized. Key words: photosynthesis, photorespiration, algae, inorganic carbon transport, transcription, translation.

scholarly journals Genomic organization, sequence analysis and expression of all five genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from tomato

1987 ◽  
Vol 209 (2) ◽  
pp. 247-256 ◽  
Author(s):  
Mamoru Sugita ◽  
Thianda Manzara ◽  
Eran Pichersky ◽  
Anthony Cashmore ◽  
Wilhelm Gruissem
Keyword(s):  
Sequence Analysis ◽  
Genomic Organization ◽  
Small Subunit ◽  
Bisphosphate Carboxylase ◽  
Genes Encoding

scholarly journals Ribonucleotide Reduction in Mycobacterium tuberculosis: Function and Expression of Genes Encoding Class Ib and Class II Ribonucleotide Reductases

2003 ◽  
Vol 71 (11) ◽  
pp. 6124-6131 ◽  
Author(s):  
Stephanie S. Dawes ◽  
Digby F. Warner ◽  
Liana Tsenova ◽  
Juliano Timm ◽  
John D. McKinney ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Class Ii ◽  
Growth Conditions ◽  
Small Subunit ◽  
Reverse Transcription Pcr ◽  
Hypoxic Conditions ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Class Ib

ABSTRACT Mycobacterium tuberculosis, the causative agent of tuberculosis, possesses a class Ib ribonucleotide reductase (RNR), encoded by the nrdE and nrdF2 genes, in addition to a putative class II RNR, encoded by nrdZ. In this study we probed the relative contributions of these RNRs to the growth and persistence of M. tuberculosis. We found that targeted knockout of the nrdF2 gene could be achieved only in the presence of a complementing allele, confirming that this gene is essential under normal, in vitro growth conditions. This observation also implied that the alternate class Ib small subunit encoded by the nrdF1 gene is unable to substitute for nrdF2 and that the class II RNR, NrdZ, cannot substitute for the class Ib enzyme, NrdEF2. Conversely, a ΔnrdZ null mutant of M. tuberculosis was readily obtained by allelic exchange mutagenesis. Quantification of levels of nrdE, nrdF2, nrdF1, and nrdZ gene expression by real-time, quantitative reverse transcription-PCR with molecular beacons by using mRNA from aerobic and O2-limited cultures showed that nrdZ was significantly induced under microaerophilic conditions, in contrast to the other genes, whose expression was reduced by O2 restriction. However, survival of the ΔnrdZ mutant strain was not impaired under hypoxic conditions in vitro. Moreover, the lungs of B6D2/F1 mice infected with the ΔnrdZ mutant had bacterial loads comparable to those of lungs infected with the parental wild-type strain, which argues against the hypothesis that nrdZ plays a significant role in the virulence of M. tuberculosis in this mouse model.

Expression of Nuclear Genes Encoding the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase

1983 ◽  
pp. 47-59 ◽  
Author(s):  
Gloria Coruzzi ◽  
Richard Broglie ◽  
Gayle Lamppa ◽  
Nam-Hai Chua
Keyword(s):  
Small Subunit ◽  
Nuclear Genes ◽  
Bisphosphate Carboxylase ◽  
Genes Encoding

Translational regulation of light-induced ribulose 1,5-bisphosphate carboxylase gene expression in amaranth

1986 ◽  
Vol 6 (7) ◽  
pp. 2347-2353
Author(s):  
J O Berry ◽  
B J Nikolau ◽  
J P Carr ◽  
D F Klessig
Keyword(s):  
Translational Regulation ◽  
Small Subunit ◽  
In Vitro Translation ◽  
Mrna Levels ◽  
Mrna Accumulation ◽  
Bisphosphate Carboxylase ◽  
Genes Encoding ◽  
Translational Level

The regulation of the genes encoding the large and small subunits of ribulose 1,5-bisphosphate carboxylase was examined in amaranth cotyledons in response to changes in illumination. When dark-grown cotyledons were transferred into light, synthesis of the large- and small-subunit polypeptides was initiated very rapidly, before any increase in the levels of their corresponding mRNAs. Similarly, when light-grown cotyledons were transferred to total darkness, synthesis of the large- and small-subunit proteins was rapidly depressed without changes in mRNA levels for either subunit. In vitro translation or in vivo pulse-chase experiments indicated that these apparent changes in protein synthesis were not due to alterations in the functionality of the mRNAs or to protein turnover, respectively. These results, in combination with our previous studies, suggest that the expression of ribulose 1,5-bisphosphate carboxylase genes can be adjusted rapidly at the translational level and over a longer period through changes in mRNA accumulation.

scholarly journals Translational regulation of light-induced ribulose 1,5-bisphosphate carboxylase gene expression in amaranth.

1986 ◽  
Vol 6 (7) ◽  
pp. 2347-2353 ◽  
Author(s):  
J O Berry ◽  
B J Nikolau ◽  
J P Carr ◽  
D F Klessig
Keyword(s):  
Translational Regulation ◽  
Small Subunit ◽  
In Vitro Translation ◽  
Mrna Levels ◽  
Mrna Accumulation ◽  
Bisphosphate Carboxylase ◽  
Genes Encoding ◽  
Translational Level

The regulation of the genes encoding the large and small subunits of ribulose 1,5-bisphosphate carboxylase was examined in amaranth cotyledons in response to changes in illumination. When dark-grown cotyledons were transferred into light, synthesis of the large- and small-subunit polypeptides was initiated very rapidly, before any increase in the levels of their corresponding mRNAs. Similarly, when light-grown cotyledons were transferred to total darkness, synthesis of the large- and small-subunit proteins was rapidly depressed without changes in mRNA levels for either subunit. In vitro translation or in vivo pulse-chase experiments indicated that these apparent changes in protein synthesis were not due to alterations in the functionality of the mRNAs or to protein turnover, respectively. These results, in combination with our previous studies, suggest that the expression of ribulose 1,5-bisphosphate carboxylase genes can be adjusted rapidly at the translational level and over a longer period through changes in mRNA accumulation.

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