Compartmentation of indole-3-acetic acid metabolism in protoplasts isolated from leaves of wild-type and IAA-overproducing transgenic tobacco plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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Lead Phytoremediation Potential of Wild Type and Transgenic Tobacco Plants

ISPEC Journal of Agricultural Sciences ◽

10.46291/ispecjasvol5iss1pp168-182 ◽

2021 ◽

Vol 5 (1) ◽

pp. 168-182

Author(s):

Hatice DAGHAN ◽

Veli UYGUR ◽

Abdullah EREN

Keyword(s):

Nicotiana Tabacum ◽

Transgenic Tobacco ◽

Transgenic Tobacco Plants ◽

Wild Type ◽

Tobacco Plants ◽

Nicotiana Tabacum L ◽

Lead Phytoremediation

Genetiği değiştirilmiş bitkiler, kurşunun (Pb) kökten yer üstü kısımlarına translokasyonunu geliştirmek için büyük bir potansiyele sahip olabilir. Transgenik olmayan ( Nicotiana tabacum L. cv. Petit Havana SR1) ve transgenik (p-cV-ChMTII GFP) tütün bitkileri tarafından Pb alımının sağlanması araştırmak için Çin hamsteri metalotiyonin II gezen bir kap deneyi yapıldı . Transgenik ve transgenik olmayan tütün bitkileri, 0, 1000, 2500, 5000 mg Pb kg- 1 ile Pb (NO 3 ) 2 olarak işlenmiş topraklarda yetiştirildi. Kelimede bir büyüme bölümünde 6 hafta boyunca çiçeklenme aşamasına kadar.Bitkilerin büyümesi, klorofil içeriği, mineral besin elementleri ve düşük glutatyon (GSH) bezleri, bitkilerin Pb alım potansiyeli ile birlikte incelenmiştir. Hem transgenik hem de transgenik olmayan bitkiler için Pb uygulamasındaki artışa bağlı olarak yer üstü biyokütle çevrildi aşamalı bir düşüş gözlendi. Yaprak besinlerinin bulaştığı, aşırı Pb işlemlerinden olumsuz etkilenmiştir, bunlardan en büyük düşüşü. Sürgün Pb yüksek derecesi 76.0 mg kg kadar ulaşan -1 transgenik ve 70.9 mg kg -1 transgenik olmayan bitkilerde. Pb alımı, p-cV-ChMTII GFP'nin tütün bitkisine aktarılmasıyla iyileştirildi; ancak, Pb fitoremediasyonunda yeterli değildi.

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Enhanced Biomass Yield of and Saccharification in Transgenic Tobacco Over-Expressing β-Glucosidase

Biomolecules ◽

10.3390/biom10050806 ◽

2020 ◽

Vol 10 (5) ◽

pp. 806

Author(s):

Eun Jin Cho ◽

Quynh Anh Nguyen ◽

Yoon Gyo Lee ◽

Younho Song ◽

Bok Jae Park ◽

...

Keyword(s):

Transgenic Tobacco ◽

Biomass Yield ◽

Thermotoga Maritima ◽

Total Biomass ◽

Transgenic Tobacco Plants ◽

Wild Type ◽

Tobacco Plants ◽

Lignocellulose Conversion ◽

Nicotiana Tabacum L ◽

Genome Transcription

Here, we report an increase in biomass yield and saccharification in transgenic tobacco plants (Nicotiana tabacum L.) overexpressing thermostable β-glucosidase from Thermotoga maritima, BglB, targeted to the chloroplasts and vacuoles. The transgenic tobacco plants showed phenotypic characteristics that were significantly different from those of the wild-type plants. The biomass yield and life cycle (from germination to flowering and harvest) of the transgenic tobacco plants overexpressing BglB were 52% higher and 36% shorter than those of the wild-type tobacco plants, respectively, indicating a change in the genome transcription levels in the transgenic tobacco plants. Saccharification in biomass samples from the transgenic tobacco plants was 92% higher than that in biomass samples from the wild-type tobacco plants. The transgenic tobacco plants required a total investment (US$/year) corresponding to 52.9% of that required for the wild-type tobacco plants, but the total biomass yield (kg/year) of the transgenic tobacco plants was 43% higher than that of the wild-type tobacco plants. This approach could be applied to other plants to increase biomass yields and overproduce β-glucosidase for lignocellulose conversion.

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Phytoremediation of 2,4-dichlorophenol using wild type and transgenic tobacco plants

Environmental Science and Pollution Research ◽

10.1007/s11356-011-0724-9 ◽

2012 ◽

Vol 19 (6) ◽

pp. 2202-2211 ◽

Cited By ~ 16

Author(s):

Melina A. Talano ◽

Débora C. Busso ◽

Cintia E. Paisio ◽

Paola S. González ◽

Silvia A. Purro ◽

...

Keyword(s):

Transgenic Tobacco ◽

Transgenic Tobacco Plants ◽

Wild Type ◽

Tobacco Plants

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Transgenic tobacco plants with improved cyanobacterial Rubisco expression but no extra assembly factors grow at near wild-type rates if provided with elevated CO2

The Plant Journal ◽

10.1111/tpj.13098 ◽

2015 ◽

Vol 85 (1) ◽

pp. 148-160 ◽

Cited By ~ 56

Author(s):

Alessandro Occhialini ◽

Myat T. Lin ◽

P. John Andralojc ◽

Maureen R. Hanson ◽

Martin A. J. Parry

Keyword(s):

Transgenic Tobacco ◽

Elevated Co2 ◽

Transgenic Tobacco Plants ◽

Wild Type ◽

Tobacco Plants ◽

Assembly Factors

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Effect of alachlor on the metabolome of wild type and GmGSTU4 overexpressing transgenic tobacco plants (Nicotiana tabacum L.)

Frontiers in Pharmacology ◽

10.3389/conf.fphar.2010.60.00144 ◽

2010 ◽

Vol 1 ◽

Cited By ~ 1

Author(s):

Irini A

Keyword(s):

Nicotiana Tabacum ◽

Transgenic Tobacco ◽

Transgenic Tobacco Plants ◽

Wild Type ◽

Tobacco Plants ◽

Nicotiana Tabacum L

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Root growth of transgenic tobacco plants with constitutive expression of the expansin gene PnEXPA3 under the action of stress factors.

Biomics ◽

10.31301/2221-6197.bmcs.2020-48 ◽

2020 ◽

Vol 12 (4) ◽

pp. 545-551

Author(s):

Z.A. Berezhneva ◽

B.R. Kuluev

Keyword(s):

Heavy Metals ◽

Transgenic Tobacco ◽

Constitutive Expression ◽

Stress Factors ◽

Cultivated Plants ◽

Transgenic Tobacco Plants ◽

Wild Type ◽

Tobacco Plants ◽

Expansin Gene ◽

Agricultural Plants

Plant α-expansin genes are of great interest for genetic engineering, since they can be used to improve the growth of the roots of agricultural plants. Previously, we obtained transgenic tobacco plants Nicotiana tabacum overexpressing the PnEXPA3 expansin gene from black poplar Populus nigra. In this work, the growth of roots of transgenic plants was studied under normal conditions and under the influence of stress factors such as hypothermia, salinity and heavy metals. Constitutive expression of the PnEXPA3 gene improved the growth of roots of transgenic tobacco plants as compared to the wild type under normal conditions and under hypothermia (+10°C), salinity (50 and 100 mM NaCl) and the action of heavy metals (200 and 400 μM acetate cadmium). The totality of the obtained data suggests that the constitutive expression of the PnEXPA3 gene can be used to improve the growth of roots of cultivated plants both under normal conditions and the action of stress factors.

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The rolB-Like Part of the Agrobacterium rhizogenes orf8 Gene Inhibits Sucrose Export in Tobacco

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2002.15.9.956 ◽

2002 ◽

Vol 15 (9) ◽

pp. 956-962 ◽

Cited By ~ 12

Author(s):

Marie Umber ◽

Lars Voll ◽

Andreas Weber ◽

Pierre Michler ◽

Léon Otten

Keyword(s):

Acetic Acid ◽

Agrobacterium Rhizogenes ◽

Mode Of Action ◽

Sucrose Transporter ◽

Naphthalene Acetic Acid ◽

Wild Type ◽

Sucrose Transport ◽

Tobacco Plants ◽

Indole 3 Acetic Acid ◽

Lines Of Evidence

Many Agrobacterium T-DNA genes belong to the highly diverse rolB family. The mode of action of most of these genes is still unknown. rolB-like sequences also are present at the 5′ ends of the T-DNA-located iaaM genes and the iaaM homolog orf8, whereas iaaM genes from Pseudomonas and Erwinia spp. lack such sequences. iaaMgenes encode tryptophan monooxygenases; these enzymes convert tryptophan into indole-3-acetamide, a precursor of indole-3-acetic acid. Tobacco plants expressing the rolB-like part of the A4 orf8 gene (2×35S-A4-Norf8 plants) accumulate glucose, fructose, sucrose, and starch and resemble sucrose transporter (NtSUT1) antisense plants. Different lines of evidence indicate that 2×35S-A4-Norf8 plants export less sucrose from source leaves. Glucose, fructose, sucrose, and starch accumulate in source leaves during sink-source transition, whereas sink tissues like petioles and midveins contain lower levels than normal. Petiole exudation experiments demonstrate a significant decrease in export of label after 14C-sucrose infiltration and after 14CO2 labeling. Grafting of stunted homozygous 2×35S-A4-Norf8 plants onto wild-type rootstocks restores growth, indicating that unloading is not affected. Growth of 2×35S-A4-Norf8 seedlings is inhibited on naphthalene acetic acid-containing media, suggesting a link between sucrose transport and auxin sensitivity.

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