Analysis of B-L?-chain gene expression in two chicken cDNA libraries

SUMMARYInfection of the snail,Biomphalaria glabrata, by the free-swimming miracidial stage of the human blood fluke,Schistosoma mansoni, and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and continued human transmission. We performed a genome-wide expression analysis of theS. mansonimiracidia and developing sporocyst using Long Serial Analysis of Gene Expression (LongSAGE). Five cDNA libraries were constructed from miracidia andin vitrocultured 6- and 20-day-old sporocysts maintained in sporocyst medium (SM) or in SM conditioned by previous cultivation with cells of theB. glabrataembryonic (Bge) cell line. We generated 21 440 SAGE tags and mapped 13 381 to theS. mansonigene predictions (v4.0e) either by estimating theoretical 3′ UTR lengths or using existing 3′ EST sequence data. Overall, 432 transcripts were found to be differentially expressed amongst all 5 libraries. In total, 172 tags were differentially expressed between miracidia and 6-day conditioned sporocysts and 152 were differentially expressed between miracidia and 6-day unconditioned sporocysts. In addition, 53 and 45 tags, respectively, were differentially expressed in 6-day and 20-day cultured sporocysts, due to the effects of exposure to Bge cell-conditioned medium.

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Myosin Heavy Chain Gene Expression in Fetal Rat Hearts after Maternal Alcohol Consumption. 351

Pediatric Research ◽

10.1203/00006450-199704001-00371 ◽

1997 ◽

Vol 41 ◽

pp. 61-61

Author(s):

Somkiat Sopontammarak ◽

Davida A Robinson ◽

Rene A Arcilla ◽

Madhu Gupta

Keyword(s):

Gene Expression ◽

Alcohol Consumption ◽

Heavy Chain ◽

Chain Gene ◽

Heavy Chain Gene ◽

Myosin Heavy Chain Gene ◽

Maternal Alcohol ◽

Maternal Alcohol Consumption ◽

Fetal Rat ◽

Rat Hearts

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Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasitic juveniles of the southern root-knot nematode Meloidogyne incognita

Nematology ◽

10.1163/156854101750236259 ◽

2001 ◽

Vol 3 (2) ◽

pp. 129-139 ◽

Cited By ~ 38

Author(s):

Jaap Bakker ◽

Fred Gommers ◽

Geert Smant ◽

Pierre Abad ◽

Marie-Noëlle Rosso ◽

...

Keyword(s):

Gene Expression ◽

Cdna Library ◽

Meloidogyne Incognita ◽

Sequence Data ◽

Homo Sapiens ◽

Large Fraction ◽

Nematode Species ◽

Cdna Libraries ◽

Parasitic Nematodes ◽

Root Knot Nematode

AbstractExpressed sequence tags (EST) have been widely used to assist in gene discovery in various organisms (e.g., Arabidopsis thaliana, Caenorhabditis elegans, Mus musculus, and Homo sapiens). In this paper we describe an EST project, which aims to investigate gene expression in Meloidogyne incognita at the onset of parasitism. Approximately 1000 5′-end sequence tags were produced from a cDNA library made of freshly hatched preparasitic second stage juveniles (J2). The EST were identified in the primary transformants of the cDNA library, and assigned to nine different functional groups, including (candidate) parasitism genes. A large fraction of the EST (45%) did not have a putative homologue in public databases. Sixty five percent of the EST that could be clustered into a functional group had putative homologues in other nematode species. EST were found for virtually all parasitism related genes that have been cloned from M. incognita to date. In addition, several novel genes were tagged, including a xylanase and a chitinase gene. The efficiency of EST projects, which produce sequence data for thousands of genes in months time without any difficult pre-selections of mRNA pools, makes random sequencing cDNA libraries a superior method to identify candidates for parasitism related genes in plant-parasitic nematodes. The sequences in this paper are retrievable from Genbank with the accession numbers BE191640 to BE191741, BE217592 to BE217720, BE225324 to BE225598, BE238852 to BE239221, and BE240829 to BE240865.

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