Assessment of genome relationships in the genus Oryza L. based on seed-protein profile analysis

1992 ◽  
Vol 85 (1) ◽  
pp. 127-132 ◽  
Author(s):  
R. Sarkar ◽  
S. N. Raina
Keyword(s):  
Seed Protein ◽  
Profile Analysis ◽  
Protein Profile ◽  
Genus Oryza

scholarly journals Root iTRAQ protein profile analysis of two Citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Huan-Xin Jiang ◽  
Lin-Tong Yang ◽  
Yi-Ping Qi ◽  
Yi-Bin Lu ◽  
Zeng-Rong Huang ◽  
...  
Keyword(s):  
Aluminum Toxicity ◽  
Profile Analysis ◽  
Protein Profile ◽  
Aluminum Tolerance ◽  
Citrus Species

scholarly journals Detection of Papillary Thyroid Carcinoma With Serum Protein Profile Analysis

2008 ◽  
Vol 134 (2) ◽  
pp. 198 ◽  
Author(s):  
William H. Moretz ◽  
Christine G. Gourin ◽  
David J. Terris ◽  
Zhong-Sheng Xia ◽  
Zhongmin Liu ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Serum Protein ◽  
Profile Analysis ◽  
Protein Profile ◽  
Papillary Thyroid ◽  
Serum Protein Profile

scholarly journals Phylogenetic relations in section Arachis based on seed protein profile

1991 ◽  
Vol 82 (5) ◽  
pp. 593-597 ◽  
Author(s):  
A. K. Singh ◽  
S. Sivaramakrishnan ◽  
Melak H. Mengesha ◽  
C. D. Ramaiah
Keyword(s):  
Seed Protein ◽  
Protein Profile ◽  
Phylogenetic Relations

Comparison of Restriction Enzyme Analysis, Arbitrarily Primed PCR, and Protein Profile Analysis Typing for Epidemiologic Investigation of an Ongoing Clostridium difficileOutbreak

1998 ◽  
Vol 36 (10) ◽  
pp. 2957-2963 ◽  
Author(s):  
Mary Ellen Rafferty ◽  
Aldona L. Baltch ◽  
Raymond P. Smith ◽  
Lawrence H. Bopp ◽  
Carol Rheal ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
General Hospital ◽  
Restriction Enzyme ◽  
Profile Analysis ◽  
Protein Profile ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Typing Methods ◽  
Arbitrarily Primed Pcr ◽  
Predominant Strain

During an outbreak of diarrhea in a general hospital in 1992, 166Clostridium difficile isolates from 102 patients were typed by restriction enzyme analysis (REA), arbitrarily primed PCR (AP-PCR), and protein profile analysis (PP) techniques. A total of 18 types and 5 subtypes were identified by REA, 32 types were identified by AP-PCR, and 9 types were identified by PP. Analysis of the data indicated the presence of a predominant strain among 76, 75, and 84% of the isolates by REA, AP-PCR, and PP, respectively. Subsequently, 45C. difficile isolates which had been collected in 1990 from 33 patients in the same hospital following a significant increase in the number of cases of diarrhea caused by C. difficile were studied by REA, AP-PCR, and PP typing techniques. Thirteen types and one subtype were identified by REA, 12 types were identified by AP-PCR, and 5 types were identified by PP. As with the isolates from 1992, a dominant strain was identified. This strain was represented by 53, 64, and 70% of the total number of isolates when the strains were typed by REA, AP-PCR, and PP, respectively. Every isolate (210 of 211) from both 1990 and 1992 that was available for typing was typeable by all three methods. Furthermore, the same dominant strain was identified in both 1990 and 1992 by each method. This study demonstrates that each of the three typing methods can be useful in epidemiologic investigations of C. difficileoutbreaks and that one strain can be dominant in an institution over a number of years.

Culture-Confirmed Reinfection of a Person with Different Strains of Borrelia burgdorferi Sensu Stricto

1998 ◽  
Vol 36 (4) ◽  
pp. 1015-1019 ◽  
Author(s):  
William T. Golde ◽  
Barbara Robinson-Dunn ◽  
Mary Grace Stobierski ◽  
Daniel Dykhuizen ◽  
Ing-Nang Wang ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Profile Analysis ◽  
Protein Profile ◽  
Erythema Migrans ◽  
Diagnostic Testing ◽  
Serum Antibody ◽  
Surface Protein ◽  
Punch Biopsy ◽  
Skin Punch Biopsy ◽  
Different Strains

In recent years, the utility of serum-based diagnostic testing for Lyme disease has improved substantially; however, recovery by culture of the bacterium from skin biopsies of suspected patients is still the only definitive laboratory test. Reinfection of patients has been assumed to occur but as yet has not been documented by serial isolates from the same person. We present a case of culture-confirmed reinfection of a patient in Menominee County, Michigan. Borrelia burgdorferi was isolated from the skin punch biopsy specimens during each episode of erythema migrans (EM) and was subjected to molecular strain typing, genetic analysis of two outer surface protein genes, protein profile analysis, and serum antibody response testing. Results show that these isolates are distinct strains of the bacterium and that the two episodes of EM were caused by independent infections. This report describes the documented, culture-confirmed reinfection of a human by two different strains of B. burgdorferi.

scholarly journals Two-dimensional gel electrophoretic protein profile analysis during seed development of Ocotea catharinensis: a recalcitrant seed species

2010 ◽  
Vol 22 (1) ◽  
pp. 23-33 ◽  
Author(s):  
Leonardo L.C. Dias ◽  
Tiago S. Balbuena ◽  
Vanildo Silveira ◽  
Claudete Santa-Catarina ◽  
Andrej Schevchenko ◽  
...  
Keyword(s):  
Seed Development ◽  
Protein Identification ◽  
Protein Extraction ◽  
Profile Analysis ◽  
Protein Profile ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Recalcitrant Seed ◽  
And Storage ◽  
Cotyledonary Stage

The aim of the present work was to characterize changes in the protein profile throughout seed development in O. catharinensis, a recalcitrant species, by two-dimensional gel electrophoresis. Protein extraction was undertaken by using a thiourea/urea buffer, followed by a precipitation step with 10% TCA. Comparative analysis during seed development showed that a large number of proteins were exclusively detected in each developmental stage. The cotyledonary stage, which represents the transition phase between embryogenesis and the beginning of metabolism related to maturation, presents the highest number of stage-specific spots. Protein identification, through MS/MS analysis, resulted in the identification of proteins mainly related to oxidative metabolism and storage synthesis. These findings contribute to a better understanding of protein metabolism during seed development in recalcitrant seeds, besides providing information on established markers that could be useful in defining and improving somatic embryogenesis protocols, besides monitoring the development of somatic embryos in this species.

scholarly journals Evaluation of biochemical and serological methods to identify and clustering yeast cells of oral Candida species by CHROMagar test, SDS-PAGE and ELISA

2004 ◽  
Vol 64 (2) ◽  
pp. 317-326 ◽  
Author(s):  
J. A. de O. Rodrigues ◽  
J. F. Höfling ◽  
F. C. A. Tavares ◽  
K. M. R. Duarte ◽  
R. B. Gonçalves ◽  
...  
Keyword(s):  
Candida Species ◽  
Profile Analysis ◽  
Protein Profile ◽  
Negative Control ◽  
Yeast Cells ◽  
Sds Page ◽  
Differential Medium ◽  
Oral Yeasts ◽  
Species Specific ◽  
High Level

The purpose of this work was to evaluate biochemical and serological methods to characterize and identify Candida species from the oral cavity. The strains used were five Candida species previously identified: C. albicans, C. guilliermondii, C. parapsilosis, C. krusei, C. tropicalis, and Kluyveromyces marxianus, as a negative control. The analyses were conducted through the SDS-PAGE associated with statistical analysis using software, chromogenic medium, and CHROMagar Candida (CA), as a differential medium for the isolation and presumptive identification of clinically important yeasts and an enzyme-linked immunoabsorbent assay (ELISA), using antisera produced against antigens from two C. albicans strains. This method enabled the screening of the three Candida species: C. albicans, C. tropicalis, and C. Krusei, with 100% of specificity. The ELISA using purified immunoglobulin G showed a high level of cross-reaction against protein extracts of Candida species. The SDS-PAGE method allowed the clustering of species-specific isolates using the Simple Matching coefficient, S SM = 1.0. The protein profile analysis by SDS-PAGE increases what is known about the taxonomic relationships among oral yeasts. This methodology showed good reproducibility and allows collection of useful information for numerical analysis on information relevant to clinical application, and epidemiological and systematical studies.

Objective Diagnosis of Cervical Cancer by Tissue Protein Profile Analysis

2011 ◽  
Author(s):  
Ajeetkumar Patil ◽  
Sujatha Bhat ◽  
Lavanya Rai ◽  
V. B. Kartha ◽  
Santhosh Chidangil ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Profile Analysis ◽  
Protein Profile ◽  
Tissue Protein
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