scholarly journals Phylogenetic relations in section Arachis based on seed protein profile

1991 ◽  
Vol 82 (5) ◽  
pp. 593-597 ◽  
Author(s):  
A. K. Singh ◽  
S. Sivaramakrishnan ◽  
Melak H. Mengesha ◽  
C. D. Ramaiah
Keyword(s):  
Seed Protein ◽  
Protein Profile ◽  
Phylogenetic Relations

Assessment of Seed Protein Quality of A Transgenic Chickpea Event Expressing Cry2Aa Protein

2020 ◽  
Author(s):  
Bidyut Kumar Sarmah ◽  
Rubi Gupta ◽  
Sumita Acharjee
Keyword(s):  
Amino Acid ◽  
Seed Protein ◽  
Protein Quality ◽  
Seed Storage Protein ◽  
Protein Profile ◽  
Seed Storage ◽  
Protein Digestibility ◽  
Amino Acid Profile ◽  
Factors Affecting

Background: Evaluation of the nutritional composition of genetically modified (GM) crops is mandatory for their deregulation. Chickpea is known for its high-quality protein and demonstrating that the seed protein quality of transgenic chickpea remains unaltered is important for its acceptance. Amino acid content, seed storage protein profile and the digestibility of chickpea protein are important determinants of seed protein quality. Thus, in the present study, we assessed the effect of Bt (Cry2Aa) gene expression on the Bt chickpea seed protein quality. Methods: We assessed the amino acid profile, in vitro protein digestibility and factors affecting protein digestibility like trypsin inhibitor, tannins and phytic acid contents of the transgenic Bt chickpea expressing a codon modified Cry2Aa gene and its non-transgenic counterpart. Furthermore, the seed storage proteins were also fractionated and separated on SDS-PAGE followed by mass spectroscopy of the major peptides. Result: Amino acid profile and factors affecting protein digestibility revealed no significant variations between transgenic and non-transgenic chickpeas. Seed storage protein profile confirmed the presence of legumin, vicilin and albumin. No potential change in the digestibility pattern of seed proteins was revealed. Our findings suggest no potential unintended changes in chickpea seed protein quality due to the expression of Cry2Aa gene.

scholarly journals Morphology and seed protein profile for a new species of the genus Cleome L. (Cleomaceae) from Pakistan

2019 ◽  
Vol 78 (1) ◽  
pp. 102-106
Author(s):  
Sana Riaz ◽  
Rubina Abid ◽  
Syed Abid Ali ◽  
Iqra Munir ◽  
Muhammad Qaiser
Keyword(s):  
New Species ◽  
Related Species ◽  
Seed Protein ◽  
Protein Profile ◽  
Seed Morphology ◽  
Closely Related Species ◽  
A New Species

Abstract A new species of the genus Cleome L. from Pakistan is described and illustrated. The new species is described under the name C. karachiensis sp. nov. and compared with two closely related species i.e., Cleome brachycarpa and C. viscosa in terms of morphology, palynology, seed morphology and seed protein profile. A key to the species of genus Cleome L. from Pakistan is also provided.

Studies on genetic diversity in chickpea utilizing morphological and total seed protein markers

2015 ◽  
Author(s):  
V. Jayalakshmi ◽  
C. Kiran Kumar Reddy ◽  
G. Jyothirmayi ◽  
A. Trivikrama Reddy
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Seed Protein ◽  
Storage Proteins ◽  
Protein Profile ◽  
Similarity Index ◽  
Morphological Characters ◽  
Morphological Markers ◽  
Protein Markers ◽  
Total Seed

Genetic diversity among seventeen chickpea varieties was investigated utilizing agro morphological traits and total seed protein markers. Morphological markers grouped all the desi genotypes (9) except ICCV 10 under Cluster I, whereas kabuli genotypes (7) were grouped in three clusters. Dendogram developed based on total seed protein profile revealed that the seventeen genotypes could be grouped into two clusters broadly at a distance of 25. Similarity index between all possible pairs of seventeen genotypes showed a range of 0.074 (Vishal and ICCV-10) to 0.579 (JAKI-9218 and ICCC 37). The correspondence between different methods might be improved by analyzing more morphological characters, storage proteins and DNA markers.

Abies seed protein profile divergent from other Pinaceae

Taxon ◽  
1991 ◽  
Vol 40 (3) ◽  
pp. 435-440 ◽  
Author(s):  
Uwe Jensen ◽  
Chen Lixue
Keyword(s):  
Seed Protein ◽  
Protein Profile

scholarly journals Value of Seed Protein Profile in the Taxonomy of cultivars of Capsicum in Nigeria

2020 ◽  
Vol 36 (2) ◽  
pp. 1-8
Author(s):  
A.O. Adepoju ◽  
A.T.J. Ogunkunle ◽  
M.A. Azeez ◽  
A.G. Femi-Adepoju
Keyword(s):  
Seed Protein ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Taxonomic Status ◽  
Polymorphic Band ◽  
Dichotomous Key ◽  
Capsicum Spp ◽  
Total Seed ◽  
Genus Capsicum

Over a decade, the taxonomy of the genus Capsicum in Nigeria  has remained largely unrevised, unclassified and unidentified. As such, there is a dearth of information on the proper identification of Capsicum spp and relatives found in the country. The aim of this study was to re-examine the taxonomic status of the Capsicum in Nigeria in order to establish genetic diversity between them for proper identification and classification. Sodium dodecyl  polyacrylamide gel electrophoresis of total seed protein was performed on five varieties of Nigerian Capsicum spp., following standard procedures. Six protein bands were observed across the five cultivars of Capsicum, of which 12-14 Kda was the only polymorphic band. Only C. fructescens var. ijosi and C. fructescens var. sombo were unique for manifesting 20-24 and 15-16 Kda bands respectively. Dendrogram of analysis obtained resolved the taxa into two distinct groups. In the first group were cultivars of C. fructescens var. ijosi and sombo while in the second group were C. chinense, which was distinctly separated from C. fructescens var. bawa and C. annum. Artificial dichotomous key was constructed for the identification of members of the genus Capsicum available in Nigeria based on the protein profiles of their seeds. Keywords: Capsicum, seed protein, electrophoresis, identification, diversity.

scholarly journals Biochemical characterization based on SDS-page analysis and correlation among traits in opium poppy (Papaver somniferum L.) germplasm

Genetika ◽  
2015 ◽  
Vol 47 (3) ◽  
pp. 1029-1050 ◽  
Author(s):  
Nidhi Verma ◽  
Sudhir Shukla ◽  
Kusum Yadav ◽  
Brij Mishra ◽  
Anu Rastogi
Keyword(s):  
Protein Content ◽  
Seed Protein ◽  
Biochemical Characterization ◽  
Seed Storage Protein ◽  
Protein Profile ◽  
Similarity Index ◽  
Papaver Somniferum ◽  
Opium Poppy ◽  
Seed Protein Content ◽  
Variable Regions

This research aimed to investigate the genetic diversity based on the pattern of seed storage protein and to establish correlation between alkaloid and seed protein content among 124 germplasm lines of opium poppy (Papaver somniferum L.). Twenty seven polypeptide bands in range of 6 to 205 kDa were recorded. Similarity index was calculated by using Jaccard?s Similarity index and cluster analysis was performed using UPGMA model. Polymorphism was observed in three variable regions i.e., high, medium and low molecular weight, among which bands of 10.4, 20, 22, 24, 30, 32, 33, 49 and 205 kDa?s were common in all the germplasms but other bands showed variation. All the 124 germplasms were broadly grouped into 13 clusters based on average linkage method. None of the trait showed significant correlation with seed protein content. The differential protein profile of the germplasms revealed wide variability and diversity among the studied germplasms which could be further used in hybridization programme to obtain maximum gain.

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