scholarly journals Phylogenetic relations in section Arachis based on seed protein profile

1991 ◽  
Vol 82 (5) ◽  
pp. 593-597 ◽  
Author(s):  
A. K. Singh ◽  
S. Sivaramakrishnan ◽  
Melak H. Mengesha ◽  
C. D. Ramaiah
Keyword(s):  
Seed Protein ◽  
Protein Profile ◽  
Phylogenetic Relations

scholarly journals Perform Cluster Analysis to Assess the Differences for Storage Seed Protein Profile in the Cowpea Mutants

2020 ◽  
Vol 9 (10) ◽  
pp. 1302-1309
Author(s):  
Madhu Choudhary ◽  
K. Ram Krishna ◽  
Rajwanti Saran
Keyword(s):  
Cluster Analysis ◽  
Seed Protein ◽  
Protein Profile ◽  
Perform Cluster Analysis

Assessment of Seed Protein Quality of A Transgenic Chickpea Event Expressing Cry2Aa Protein

2020 ◽  
Author(s):  
Bidyut Kumar Sarmah ◽  
Rubi Gupta ◽  
Sumita Acharjee
Keyword(s):  
Amino Acid ◽  
Seed Protein ◽  
Protein Quality ◽  
Seed Storage Protein ◽  
Protein Profile ◽  
Seed Storage ◽  
Protein Digestibility ◽  
Amino Acid Profile ◽  
Factors Affecting

Background: Evaluation of the nutritional composition of genetically modified (GM) crops is mandatory for their deregulation. Chickpea is known for its high-quality protein and demonstrating that the seed protein quality of transgenic chickpea remains unaltered is important for its acceptance. Amino acid content, seed storage protein profile and the digestibility of chickpea protein are important determinants of seed protein quality. Thus, in the present study, we assessed the effect of Bt (Cry2Aa) gene expression on the Bt chickpea seed protein quality. Methods: We assessed the amino acid profile, in vitro protein digestibility and factors affecting protein digestibility like trypsin inhibitor, tannins and phytic acid contents of the transgenic Bt chickpea expressing a codon modified Cry2Aa gene and its non-transgenic counterpart. Furthermore, the seed storage proteins were also fractionated and separated on SDS-PAGE followed by mass spectroscopy of the major peptides. Result: Amino acid profile and factors affecting protein digestibility revealed no significant variations between transgenic and non-transgenic chickpeas. Seed storage protein profile confirmed the presence of legumin, vicilin and albumin. No potential change in the digestibility pattern of seed proteins was revealed. Our findings suggest no potential unintended changes in chickpea seed protein quality due to the expression of Cry2Aa gene.

scholarly journals Morphology and seed protein profile for a new species of the genus Cleome L. (Cleomaceae) from Pakistan

2019 ◽  
Vol 78 (1) ◽  
pp. 102-106
Author(s):  
Sana Riaz ◽  
Rubina Abid ◽  
Syed Abid Ali ◽  
Iqra Munir ◽  
Muhammad Qaiser
Keyword(s):  
New Species ◽  
Related Species ◽  
Seed Protein ◽  
Protein Profile ◽  
Seed Morphology ◽  
Closely Related Species ◽  
A New Species

Abstract A new species of the genus Cleome L. from Pakistan is described and illustrated. The new species is described under the name C. karachiensis sp. nov. and compared with two closely related species i.e., Cleome brachycarpa and C. viscosa in terms of morphology, palynology, seed morphology and seed protein profile. A key to the species of genus Cleome L. from Pakistan is also provided.

Studies on genetic diversity in chickpea utilizing morphological and total seed protein markers

2015 ◽  
Author(s):  
V. Jayalakshmi ◽  
C. Kiran Kumar Reddy ◽  
G. Jyothirmayi ◽  
A. Trivikrama Reddy
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Seed Protein ◽  
Storage Proteins ◽  
Protein Profile ◽  
Similarity Index ◽  
Morphological Characters ◽  
Morphological Markers ◽  
Protein Markers ◽  
Total Seed

Genetic diversity among seventeen chickpea varieties was investigated utilizing agro morphological traits and total seed protein markers. Morphological markers grouped all the desi genotypes (9) except ICCV 10 under Cluster I, whereas kabuli genotypes (7) were grouped in three clusters. Dendogram developed based on total seed protein profile revealed that the seventeen genotypes could be grouped into two clusters broadly at a distance of 25. Similarity index between all possible pairs of seventeen genotypes showed a range of 0.074 (Vishal and ICCV-10) to 0.579 (JAKI-9218 and ICCC 37). The correspondence between different methods might be improved by analyzing more morphological characters, storage proteins and DNA markers.

Assessment of genome relationships in the genus Oryza L. based on seed-protein profile analysis

1992 ◽  
Vol 85 (1) ◽  
pp. 127-132 ◽  
Author(s):  
R. Sarkar ◽  
S. N. Raina
Keyword(s):  
Seed Protein ◽  
Profile Analysis ◽  
Protein Profile ◽  
Genus Oryza

scholarly journals Biochemical characterization based on SDS-page analysis and correlation among traits in opium poppy (Papaver somniferum L.) germplasm

Genetika ◽  
2015 ◽  
Vol 47 (3) ◽  
pp. 1029-1050 ◽  
Author(s):  
Nidhi Verma ◽  
Sudhir Shukla ◽  
Kusum Yadav ◽  
Brij Mishra ◽  
Anu Rastogi
Keyword(s):  
Protein Content ◽  
Seed Protein ◽  
Biochemical Characterization ◽  
Seed Storage Protein ◽  
Protein Profile ◽  
Similarity Index ◽  
Papaver Somniferum ◽  
Opium Poppy ◽  
Seed Protein Content ◽  
Variable Regions

This research aimed to investigate the genetic diversity based on the pattern of seed storage protein and to establish correlation between alkaloid and seed protein content among 124 germplasm lines of opium poppy (Papaver somniferum L.). Twenty seven polypeptide bands in range of 6 to 205 kDa were recorded. Similarity index was calculated by using Jaccard?s Similarity index and cluster analysis was performed using UPGMA model. Polymorphism was observed in three variable regions i.e., high, medium and low molecular weight, among which bands of 10.4, 20, 22, 24, 30, 32, 33, 49 and 205 kDa?s were common in all the germplasms but other bands showed variation. All the 124 germplasms were broadly grouped into 13 clusters based on average linkage method. None of the trait showed significant correlation with seed protein content. The differential protein profile of the germplasms revealed wide variability and diversity among the studied germplasms which could be further used in hybridization programme to obtain maximum gain.

scholarly journals Evaluation of Genetic Diversity of Seven Species of Solanum in Nigeria Using SDS-PAGE

2019 ◽  
pp. 1-6
Author(s):  
Busirat Odunayo Ogundairo ◽  
Muyiwa Segun Ayodele
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Seed Protein ◽  
Protein Profile ◽  
Generic Status ◽  
Molecular Weights ◽  
Sds Page ◽  
The Family ◽  
Intraspecific Relationships ◽  
Poly Acrylamide

Seed protein profile of 12 accessions belonging to 7 species of the genus Solanum of the family solanaceae were investigated through poly acrylamide gel electrophoresis. The accessions were selected based on their overlapping morphological associations observed on the field. Occurrence of two protein bands of molecular weights 100 kda and 20 kda in all accession showed interspecific relationships and could be used as generic bands among the genus Solanum. All morphologically similar accessions manifested similarities in their protein bands except S. melongena. Dendrogram based on upgma also revealed the generic status and intraspecific relationships of the accession of S. aethiopicum and S. macrocarpon.

scholarly journals Revealing Antioxidant and Antidiabetic Potency of Melinjo (Gnetum Gnemon) Seed Protein Hydrolysate at Different Stages of Seed Maturation

2019 ◽  
Vol 7 (2) ◽  
pp. 479-487 ◽  
Author(s):  
Anang Supriyadi ◽  
Laras Sekar Arum ◽  
Ari Satia Nugraha ◽  
Anak Agung Istri Ratnadewi ◽  
Tri Agus Siswoyo
Keyword(s):  
Seed Protein ◽  
Protein Hydrolysate ◽  
Protein Profile ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antidiabetic Activity ◽  
Degree Of Hydrolysis ◽  
Protein Extract ◽  
Sds Page ◽  
Gnetum Gnemon

roteins hydrolyzed from melinjo seeds (Gnetum gnemon) at green (GM), yellow (YM) and red (RM) stages of maturity were studied for their effectiveness in antioxidant and antidiabetic activities. The seed protein extract was hydrolyzed using alcalase 2.4L, and the resulting hydrolysates with the highest degree of hydrolysis, protein profile, and the most potent contributors to antioxidant and invitro antidiabetic activities were identified. The degree of hydrolysis value of hydrolysates ranged from 52-84%, and the SDS-PAGE protein profile showed two distinct bands in which the band with molecular weight of 30 kDa degraded more intensively. Antioxidant capacity was measured using different standard methods, including radical cation 2,2-azinobis-(3-ethylbenzothizoline-6-sulphonate) (ABTS•+) assay, hydroxyl radical (OH•), and superoxide anion (O2•-) scavenging. The green hydrolysate (GMH) had significantly higher (p<0.05) free radical scavenging (ABTS•+, OH•, and O2•-) activities than that of the yellow hydrolysate (YMH) and red hydrolysate (RMH). However, invitro antidiabetic testing was performed based on the inhibitory activity of α-amylase and α-glucosidase. GMH was found to be more effective than YMH and RMH. These results showed that the antioxidant and antidiabetic activity in hydrolyzed GM protein has high potential to be utilized as natural nutraceuticals.

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