Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars

1993 ◽  
Vol 85-85 (6-7) ◽  
pp. 697-701 ◽  
Author(s):  
J. I. Stiles ◽  
C. Lemme ◽  
S. Sondur ◽  
M. B. Morshidi ◽  
R. Manshardt
Keyword(s):  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna

scholarly journals Genetic Distance and Diversity in Table Beet and Sugar Beet Accessions Measured by Randomly Amplified Polymorphic DNA

1999 ◽  
Vol 124 (6) ◽  
pp. 630-635 ◽  
Author(s):  
Min Wang ◽  
Irwin L. Goldman
Keyword(s):  
Sugar Beet ◽  
Genetic Distance ◽  
Beta Vulgaris ◽  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna ◽  
Breeding Lines ◽  
University Of Wisconsin ◽  
Swiss Chard ◽  
The University ◽  
Standard Table

Genetic relationships among 37 accessions of Beta vulgaris, including 21 table beet, 14 sugar beet, and two Swiss chard (Beta vulgaris ssp. cicla) accessions, were evaluated using randomly amplified polymorphic DNA (RAPD). Genetic distance was estimated based on the presence or absence of polymorphic RAPD bands. Multidimensional scaling plots of genetic distance values revealed that table beet inbred lines from the University of Wisconsin Table beet Breeding Program clustered in an intermediate position between sugar beet breeding lines and standard table beet germplasm, likely because of their origin from an introgression program designed to incorporate sugar beet genes.

scholarly journals DNA Fingerprinting to Identify Nine Anthurium Pot Plant Cultivars and Examine Their Genetic Relationship

HortScience ◽  
2001 ◽  
Vol 36 (4) ◽  
pp. 758-760 ◽  
Author(s):  
D.G. Ranamukhaarachchi ◽  
R.J. Henny ◽  
C.L. Guy ◽  
Q.B. Li
Keyword(s):  
Genetic Relationship ◽  
Rapd Markers ◽  
Parental Species ◽  
Genetic Relationships ◽  
Dna Fingerprints ◽  
Randomly Amplified Polymorphic Dna ◽  
Arbitrary Primers ◽  
D Values ◽  
High Degree ◽  
Pot Plant

Randomly amplified polymorphic DNA (RAPD) markers were utilized to determine the genetic relationships of nine morphologically similar pot plant cultivars of Anthurium sp. by developing DNA fingerprints (DFP). Of 25 arbitrary primers screened, nine generated DFPs that were used in computing the genetic distance (d) and similarity coefficient (C) values. All cultivars tested exhibited a high degree of genetic similarity. `Lady Ann' and `Lady Beth' possessed the closest relationship with d and C values of 0.06 and 0.98, respectively. The next closest genetic relationship was between `Red Hot' and `Southern Blush' (d = 0.33, C = 0.89). These two cultivars exhibited a more distant relationship to the other seven cultivars as indicated by higher `d' values. However, this study showed that the nine Anthurium cultivars examined were genetically closely related. These cultivars share specific DNA bands with three possible parental species (A. andraeanum Linden ex Andre, A. antioquens L., and A. amnicola Dressler) included in this study, which may indicate similarities in their pedigree. This study shows that RAPDs can be a useful tool to distinguish Anthurium pot plant cultivars as well as identify their genetic relationships.

scholarly journals Genetic Relationships of Pyrus Species and Cultivars Native to East Asia Revealed by Randomly Amplified Polymorphic DNA Markers

2002 ◽  
Vol 127 (2) ◽  
pp. 262-270 ◽  
Author(s):  
Yuanwen Teng ◽  
Kenji Tanabe ◽  
Fumio Tamura ◽  
Akihiro Itai
Keyword(s):  
East Asia ◽  
Clustering Analysis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna ◽  
Pyrus Calleryana ◽  
Chinese White ◽  
Species Specific ◽  
Kochi Prefecture

A total of 118 Pyrus sp. (pear) and cultivars native mainly to east Asia were subjected to randomly amplified polymorphic DNA (RAPD) analysis to evaluate genetic variation and relationships among the accessions. Two hundred fifty RAPD markers were scored from 20 decamer primers. RAPD markers specific to species were identified. Clustering analysis revealed two divisions: one comprising cultivars of P. communis L., and the other including all accessions of Pyrus native to east Asia. The grouping of the species and cultivars by RAPD data largely agrees with morphological pear taxonomy. However, some noted incongruence existed between two classification methods. Pyrus calleryana Dcne. clustered together with P. koehnei Schneid., P. fauriei Schneid. and P. dimorphophylla Makino. Pyrus betulaefolia Bge. clustered with P. ×hopeiensis Yu and P. ×phaeocarpa Rehd. A noncultivated clone of P. aromatica Kikuchi et Nakai grouped with P. aromatica cultivars. Pyrus hondoensis Nakai et Kikuchi and cultivars of P. ussuriensis Max. formed a single group. Some accessions from Korea (named Korean pear) had species-specific RAPD markers and comprised an independent group. Most of the Chinese white pears clustered together with most of the Chinese sand pears. Based on the present results, the new nomenclature P. pyrifolia var. sinensis (Lindley) Teng et Tanabe for Chinese white pear was suggested. Most accessions of Japanese pears fell into one main group, whereas pear cultivars from Kochi Prefecture of Japan subclustered with some Chinese sand pears and one accession from Korea. Our results infer that some local Japanese pear cultivar populations may have been derived from cultivars native to Kochi Prefecture in Shikoku region, and that the latter may have been introduced from ancient China and/or Korea.

The use of 17 RAPD primers in some of Iranian sheep breeds

2003 ◽  
Vol 2003 ◽  
pp. 144-144
Author(s):  
M. Tahmoorespur ◽  
M. R. Nassiry ◽  
A. Mohammady
Keyword(s):  
Genome Mapping ◽  
Genetic Relationships ◽  
Rapd Marker ◽  
Marker Development ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Sheep Breeds ◽  
Livestock Species ◽  
Target Sites ◽  
Polymerase Chain

The randomly amplified polymorphic DNA technique was developed by Williams et al,(1990) and Welsh and McClelland et al.(1990). The technique is based on polymerase chain reaction using primers homologous to random target sites in the genome. The main advantages of RAPD assays are that it is simple, less labour intensive, comparatively less expensive and safe than other methods. The RAPD hase been used for various applications including species identification, establishing genetic relationships, estimating genetic diversity and genome mapping in various livestock species including sheep. These studies reflected the effectiveness of rapd as potential genetic marker. The objective of the current study was to investigate RAPD marker development to distinguish genetic differences and similarity between and within some Iranian sheep breeds.

scholarly journals Detection of genetic relationships among fourArtemia species using randomly amplified polymorphic DNA (RAPD)

1999 ◽  
Vol 8 (4) ◽  
pp. 383-383
Author(s):  
Yi Sun ◽  
Yi-Cheng Zhong ◽  
Wen-Qin Song ◽  
Run-Sheng Zhang ◽  
Rui-Yang Chen ◽  
...  
Keyword(s):  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna

scholarly journals Identifying Blueberry Cultivars and Evaluating Their Genetic Relationships Using Randomly Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat- (SSR-) anchored Primers

1997 ◽  
Vol 122 (1) ◽  
pp. 74-78 ◽  
Author(s):  
A. Levi ◽  
L.J. Rowland
Keyword(s):  
Genetic Similarity ◽  
Genetic Relationships ◽  
Vaccinium Corymbosum ◽  
Similarity Matrix ◽  
Randomly Amplified Polymorphic Dna ◽  
Pedigree Data ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain ◽  
Simple Sequence

Fifteen highbush (or highbush hybrid) blueberry cultivars (Vaccinium corymbosum Linnaeus), two rabbiteye blueberry cultivars (V. ashei Reade), and one southern lowbush (V. darrowi Camp) selection from the wild were examined using seventeen 10-base RAPD and seven 15- to 18-base SSR-anchored primers (primers comprised of SSR motifs) in polymerase chain reactions (PCRs). Fifteen RAPD and three SSR markers resulting from these reactions were chosen to construct a DNA fingerprinting table to distinguish among the genotypes included in this study. Similarity values were calculated based on 132 RAPD and 51 SSR bands, and a dendrogram was constructed based on the similarity matrix. The V. ashei cultivars and V. darrowi selection grouped out separately from the V. corymbosum cultivars as expected. However, estimates of relative genetic similarity between genotypes within the V. corymbosum group did not agree well with known pedigree data and, thus, indicated that RAPD and SSR data did not accurately assess the genetic relationships of cultivars within this species.

scholarly journals Identification of Poinsettia Cultivars Using RAPD Markers

HortScience ◽  
1997 ◽  
Vol 32 (1) ◽  
pp. 122-124 ◽  
Author(s):  
Jing-Tian Ling ◽  
Roger Sauve ◽  
Nick Gawel
Keyword(s):  
Cluster Analysis ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
The Other ◽  
Euphorbia Pulcherrima ◽  
Randomly Amplified Polymorphic Dna ◽  
Upgma Cluster Analysis ◽  
Leaf Tissues ◽  
Components Analysis

Randomly amplified polymorphic DNA (RAPD) techniques were used to compare the DNA from leaf tissues of nine commercial poinsettia (Euphorbia pulcherrima Wild ex Klotzsch) cultivars. Amplification occurred in 57 out of 60 (95%) tested primers. Nine primers that revealed polymorphisms among cultivars were selected for further evaluation. Forty-eight RAPD bands were scored from these primers, and 33 (69%) were polymorphic. All tested cultivars could be discriminated with seven bands generated from primers OPB7 and OPC13. Results of a UPGMA cluster analysis and principal components analysis placed the nine cultivars into two groups: one group consisted of `Jingle Bells', `Supjibi', and `V-17 Angelika', the other of `V-14 Glory', `Red Sails', `Jolly Red', and `Freedom'. `Lilo Red' and `Pink Peppermint' belonged to the latter group, but were relatively distant from other cultivars in that group. These results indicate that RAPDs are efficient for identification of poinsettia cultivars and for determination of the genetic relationships among cultivars.

Fingerprinting of cauliflower cultivars using RAPD markers

2004 ◽  
Vol 55 (2) ◽  
pp. 117 ◽  
Author(s):  
Ida A. Astarini ◽  
Julie A. Plummer ◽  
Rachel A. Lancaster ◽  
Guijun Yan
Keyword(s):  
Phylogenetic Analysis ◽  
Brassica Oleracea ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna ◽  
Routine Identification ◽  
Polymorphic Bands

Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 cultivars of cauliflower (Brassica oleracea var. botrytis L.). Forty decamer primers were examined, among which 15 primers produced polymorphism. Twenty-five polymorphic bands were observed, ranging in size from 428 to 1646 bp. A fingerprinting key was generated using these polymorphic bands. A dendogram was constructed using neighbour-joining analysis based on phylogenetic analysis using parsimony (PAUP). Results indicate that RAPD markers can be used for the routine identification of cauliflower cultivars within B. oleracea var. botrytis L.

scholarly journals RAPD analysis of genetic relatedness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Turkey

2011 ◽  
Vol 38 (No. 4) ◽  
pp. 134-141 ◽  
Author(s):  
S. Bayazit ◽  
B. Imrak ◽  
A. Küden ◽  
M. Kemal Güngör
Keyword(s):  
Rapd Markers ◽  
Genetic Relatedness ◽  
Geographical Origin ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna ◽  
Cydonia Oblonga ◽  
A Minor ◽  
Almost All ◽  
Different Parts

Quince (Cydonia oblonga Mill.) is a minor fruit crop, which is primarily used for marmalade, jam, sauce and as rootstocks for pears. Different cultivated and local quince genotypes are grown in almost all parts of Turkey for fruit usage. In this study, randomly amplified polymorphic DNA (RAPD) technology was used to study the genetic relationships among 13 quince accessions selected from different parts of Turkey. Thirty decamer primers were used and 14 of them did not produce any polymorphism. The remaining 16 primers ranged in their amplification fragments between one (P-402, P-437, OPA 10, OPA 16, OPA 18 and OPA-19) and five (OPA-06 and OPA-07). The size of fragments varied from 100 to 1500 bp. Similarity values among the studied genotypes ranged between 0.483 and 0.925. The resulting dendrogram clustered into two groups (0.69 similarity value) based on evaluation of genetic similarities and differences. The results suggest that RAPD analysis could be used to distinguish and determine genetic variation among quince accessions. Also, the obtained clustering based on RAPD markers agreed to some extent with the geographical origin of the studied set of quince accessions.

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