Partial inactivation of wheat nucleolus organisers by the nucleolus organiser chromosomes from Aegilops umbellulata

Chromosoma ◽  
1982 ◽  
Vol 84 (5) ◽  
pp. 687-700 ◽  
Author(s):  
G. Martini ◽  
M. O'Dell ◽  
R. B. Flavell
Keyword(s):  
Aegilops Umbellulata ◽  
Nucleolus Organiser ◽  
Partial Inactivation

Partial Inactivation of Angiotensin II by Plasmin

1969 ◽  
Vol 21 (01) ◽  
pp. 166-167
Author(s):  
J Prokopowicz ◽  
K Worowski ◽  
S Niewiarowski
Keyword(s):  
Angiotensin Ii ◽  
Partial Inactivation

scholarly journals Ragged N-termini and other variants of class A β-lactamases analysed by chromatofocusing

1991 ◽  
Vol 273 (3) ◽  
pp. 503-510 ◽  
Author(s):  
A Matagne ◽  
B Joris ◽  
J Van Beeumen ◽  
J M Frère
Keyword(s):  
Experimental Error ◽  
Catalytic Properties ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Gram Positive Bacteria ◽  
Class A ◽  
Partial Inactivation ◽  
Asparagine Residue

Four beta-lactamases excreted by Gram-positive bacteria exhibited microheterogeneity when analysed by chromatofocusing or ion-exchange chromatography. Ragged N-termini were in part responsible for the charge variants, but deamidation of an asparagine residue was also involved, at least for the Bacillus licheniformis enzyme. The activity of a contaminating proteinase could also be demonstrated in the case of Actinomadura R39 beta-lactamase. With that enzyme, proteolysis resulted in partial inactivation, but the inactivated fragments were easily separated from the active forms. With these, as with the other enzymes, the kinetic parameters of the major variants were identical with those of the mixture within the limits of experimental error, so that the catalytic properties of these enzymes can be determined with the ‘heterogeneous’ preparations.

Influence of partial inactivation on growth of Listeria monocytogenes under sub-optimal conditions of increased NaCl concentration or increased acidity

2009 ◽  
Vol 10 (2) ◽  
pp. 267-271 ◽  
Author(s):  
Andreja Rajkovic ◽  
Mieke Uyttendaele ◽  
Nancy Van Houteghem ◽  
Sandra Maria Osés Gómez ◽  
Johan Debevere ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Optimal Conditions ◽  
Nacl Concentration ◽  
Partial Inactivation

Association of Storage Protein Patterns and Leaf Rust Resistance Derived From Aegilops umbellulata Zhuk. in the Wheat

1998 ◽  
Vol 26 (4) ◽  
pp. 471-478
Author(s):  
Tsvetana Stoilova ◽  
Antoaneta Yankova
Keyword(s):  
Leaf Rust ◽  
Storage Protein ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Protein Patterns ◽  
Aegilops Umbellulata

scholarly journals Miltefosine Affects Lipid Metabolism in Leishmania donovani Promastigotes

2007 ◽  
Vol 51 (4) ◽  
pp. 1425-1430 ◽  
Author(s):  
M. Rakotomanga ◽  
S. Blanc ◽  
K. Gaudin ◽  
P. Chaminade ◽  
P. M. Loiseau
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Leishmania Donovani ◽  
Cholesterol Content ◽  
Strong Reduction ◽  
Drug Pressure ◽  
Antileishmanial Drug ◽  
Partial Inactivation ◽  
Orally Active

ABSTRACT Miltefosine (hexadecylphosphocholine [HePC]) is the first orally active antileishmanial drug. Transient HePC treatment of Leishmania donovani promastigotes at 10 μM significantly reduced the phosphatidylcholine content and enhanced the phosphatidylethanolamine (PE) content in parasite membranes, suggesting a partial inactivation of PE-N-methyltransferase. Phospholipase D activity did not seem to be affected by HePC. In addition, the enhancement of the lysophosphatidylcholine content could be ascribed to phospholipase A2 activation. Moreover, transient HePC treatment had no effect on the fatty acid alkyl chain length or the fatty acid unsaturation rate. Concerning sterols, we found a strong reduction of the C24 alkylated sterol content, and the enhancement of the cholesterol content could be the result of the HePC condensation effect with sterols. Because some of the effects observed after transient HePC treatment were different from those previously observed in HePC-resistant parasites, it could be hypothesized that continuous in vitro drug pressure induces the mechanisms of regulation in Leishmania lipid metabolism.

scholarly journals Regulation of the thyroid NADPH-dependent H2O2 generator by Ca2+: studies with phenylarsine oxide in thyroid plasma membrane

1997 ◽  
Vol 321 (2) ◽  
pp. 383-388 ◽  
Author(s):  
Yves GORIN ◽  
Anne Marie LESENEY ◽  
Renée OHAYON ◽  
Corinne DUPUY ◽  
Jacques POMMIER ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Plasma Membranes ◽  
Direct Action ◽  
Native Enzyme ◽  
Thiol Groups ◽  
Phenylarsine Oxide ◽  
Oxidoreductase Activity ◽  
H2o2 Formation ◽  
Partial Inactivation ◽  
Dose Dependent

Pig thyroid plasma membranes contain a Ca2+-dependent NADPH:O2 oxidoreductase, the thyroid NADPH-dependent H2O2 generator. This provided the H2O2 for the peroxidase-catalysed synthesis of thyroid hormones. The effect of the tervalent arsenical, phenylarsine oxide (PAO), on the NADPH oxidase was studied. PAO caused two directly related dose-dependent effects with similar half-effect concentrations of PAO (3 nmol of PAO/mg of protein): (i) partial inactivation of H2O2 formation by the Ca2+-stimulated enzyme, and (ii) desensitization of the enzyme activity to Ca2+. PAO had no effect on membranes that had been Ca2+-desensitized by α-chymotrypsin treatment. The NADPH oxidase in membranes treated with excess PAO had the same Vmax with and without Ca2+. This value was half the Vmax of the native enzyme. However, the Km for NADPH determined with Ca2+ (18 ƁM, identical with that of the native enzyme) was approx. one-third of the Km measured without Ca2+, showing the direct action of Ca2+ on the PAOŐenzyme complex. PAO had the same effects, partial inactivation and Ca2+ desensitization, on the NADPH:ferricyanide oxidoreductase activity of the NADPH oxidase, suggesting that PAO acts on the flavodehydrogenase entity of the enzyme. Both partial inactivation and Ca2+ desensitization were completely and specifically reversed by 2,3-dimercaptopropanol, partly reversed by dithiothreitol and not reversed by 2-mercaptoethanol, indicating that PAO binds to vicinal thiol groups. These results suggest that thiol groups are involved in the control of thyroid NADPH oxidase by Ca2+; PAO bound to vicinal thiols might alter the structure of the enzyme so that electron transfer occurs without Ca2+ but more slowly.

scholarly journals Correction: Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
Author(s):  
István Molnár ◽  
Marie Kubaláková ◽  
Hana Å imková ◽  
András Cseh ◽  
Márta Molnár-Láng ◽  
...  
Keyword(s):  
Aegilops Umbellulata ◽  
Flow Sorting ◽  
Chromosome Isolation

The Au family, a novel short interspersed element (SINE) from Aegilops umbellulata

2001 ◽  
Vol 102 (4) ◽  
pp. 463-470 ◽  
Author(s):  
Y. Yasui ◽  
S. Nasuda ◽  
Y. Matsuoka ◽  
T. Kawahara
Keyword(s):  
Aegilops Umbellulata

Transfer of leaf rust and stripe rust resistance from Aegilops umbellulata Zhuk. to bread wheat (Triticum aestivum L.)

2007 ◽  
Vol 55 (6) ◽  
pp. 849-859 ◽  
Author(s):  
Parveen Chhuneja ◽  
Satinder Kaur ◽  
R. K. Goel ◽  
M. Aghaee-Sarbarzeh ◽  
M. Prashar ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Leaf Rust ◽  
Bread Wheat ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Triticum Aestivum L ◽  
Stripe Rust Resistance ◽  
Aegilops Umbellulata

Transfer of human genes conferring resistance to methylating mutagens, but not to UV irradiation and cross-linking agents, into Chinese hamster ovary cells

1987 ◽  
Vol 7 (5) ◽  
pp. 2024-2030
Author(s):  
B Kaina ◽  
A A Van Zeeland ◽  
C Backendorf ◽  
H W Thielmann ◽  
P Van de Putte
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Human Fibroblasts ◽  
Chinese Hamster ◽  
Native Form ◽  
Ovary Cells ◽  
Human Dna ◽  
Human Genes ◽  
Partial Inactivation ◽  
High Doses

Chinese hamster ovary cells were transfected by human DNA ligated to the bacterial gpt (xanthine-guanine-phosphoribosyltransferase) gene which was used either in its native form or after partial inactivation with methylnitrosourea. The gpt+ transfectants were screened for resistance to high doses of N-methyl-N'-nitro-N-nitrosoguanidine. Using this approach, we showed that Chinese hamster ovary cells can acquire N-methyl-N'-nitro-N-nitrosoguanidine resistance upon transfection with DNA from diploid human fibroblasts, that this resistance is transferable by secondary transfection and is specific for methylating mutagens, and that it is not caused by increased removal of O6-methylguanine, 3-methyladenine, and 7-methylguanine from DNA.

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