Molecular analysis of the Escherichia coli has gene encoding a DNA-binding protein, which preferentially recognizes curved DNA sequences

1991 ◽  
Vol 230 (1-2) ◽  
pp. 332-336 ◽  
Author(s):  
Hisami Yamada ◽  
Takayuki Yoshida ◽  
Ken-ichi Tanaka ◽  
Chihiro Sasakawa ◽  
Takeshi Mizuno
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Molecular Analysis ◽  
Dna Sequences ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Gene Encoding ◽  
Curved Dna

Growth Phase-Dependent Variation in Protein Composition of the Escherichia coli Nucleoid

1999 ◽  
Vol 181 (20) ◽  
pp. 6361-6370 ◽  
Author(s):  
Talukder Ali Azam ◽  
Akira Iwata ◽  
Akiko Nishimura ◽  
Susumu Ueda ◽  
Akira Ishihama
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Stationary Phase ◽  
Binding Protein ◽  
Protein A ◽  
Dna Binding Protein ◽  
Host Factor ◽  
Late Stationary Phase ◽  
E Coli ◽  
Curved Dna

ABSTRACT The genome DNA of Escherichia coli is associated with about 10 DNA-binding structural proteins, altogether forming the nucleoid. The nucleoid proteins play some functional roles, besides their structural roles, in the global regulation of such essential DNA functions as replication, recombination, and transcription. Using a quantitative Western blot method, we have performed for the first time a systematic determination of the intracellular concentrations of 12 species of the nucleoid protein in E. coli W3110, including CbpA (curved DNA-binding protein A), CbpB (curved DNA-binding protein B, also known as Rob [right origin binding protein]), DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Qβ), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid protein), IciA (inhibitor of chromosome initiation A), IHF (integration host factor), Lrp (leucine-responsive regulatory protein), and StpA (suppressor oftd mutant phenotype A). Intracellular protein levels reach a maximum at the growing phase for nine proteins, CbpB (Rob), DnaA, Fis, Hfq, H-NS, HU, IciA, Lrp, and StpA, which may play regulatory roles in DNA replication and/or transcription of the growth-related genes. In descending order, the level of accumulation, calculated in monomers, in growing E. coli cells is Fis, Hfq, HU, StpA, H-NS, IHF*, CbpB (Rob), Dps*, Lrp, DnaA, IciA, and CbpA* (stars represent the stationary-phase proteins). The order of abundance, in descending order, in the early stationary phase is Dps*, IHF*, HU, Hfq, H-NS, StpA, CbpB (Rob), DnaA, Lrp, IciA, CbpA, and Fis, while that in the late stationary phase is Dps*, IHF*, Hfq, HU, CbpA*, StpA, H-NS, CbpB (Rob), DnaA, Lrp, IciA, and Fis. Thus, the major protein components of the nucleoid change from Fis and HU in the growing phase to Dps in the stationary phase. The curved DNA-binding protein, CbpA, appears only in the late stationary phase. These changes in the composition of nucleoid-associated proteins in the stationary phase are accompanied by compaction of the genome DNA and silencing of the genome functions.

scholarly journals A temperature-sensitive single-stranded DNA-binding protein from Escherichia coli.

1980 ◽  
Vol 255 (7) ◽  
pp. 2897-2901
Author(s):  
R.R. Meyer ◽  
J. Glassberg ◽  
J.V. Scott ◽  
A. Kornberg
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Temperature Sensitive ◽  
Single Stranded Dna

scholarly journals The major histocompatibility complex class II promoter-binding protein RFX (NF-X) is a methylated DNA-binding protein.

1993 ◽  
Vol 13 (11) ◽  
pp. 6810-6818 ◽  
Author(s):  
X Y Zhang ◽  
N Jabrane-Ferrat ◽  
C K Asiedu ◽  
S Samac ◽  
B M Peterlin ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Dna Binding ◽  
Dna Sequences ◽  
Protein Complexes ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Class Ii ◽  
Major Histocompatibility ◽  
Methylated Dna ◽  
Mammalian Protein

A mammalian protein called RFX or NF-X binds to the X box (or X1 box) in the promoters of a number of major histocompatibility (MHC) class II genes. In this study, RFX was shown to have the same DNA-binding specificity as methylated DNA-binding protein (MDBP), and its own cDNA was found to contain a binding site for MDBP in the leader region. MDBP is a ubiquitous mammalian protein that binds to certain DNA sequences preferentially when they are CpG methylated and to other related sequences, like the X box, irrespective of DNA methylation. MDBP from HeLa and Raji cells formed DNA-protein complexes with X-box oligonucleotides that coelectrophoresed with those containing standard MDBP sites. Furthermore, MDBP and X-box oligonucleotides cross-competed for the formation of these DNA-protein complexes. DNA-protein complexes obtained with MDBP sites displayed the same partial supershifting with an antiserum directed to the N terminus of RFX seen for complexes containing an X-box oligonucleotide. Also, the in vitro-transcribed-translated product of a recombinant RFX cDNA bound specifically to MDBP ligands and displayed the DNA methylation-dependent binding of MDBP. RFX therefore contains MDBP activity and thereby also EF-C, EP, and MIF activities that are indistinguishable from MDBP and that bind to methylation-independent sites in the transcriptional enhancers of polyomavirus and hepatitis B virus and to an intron of c-myc.

scholarly journals Molecular Analysis of BcrR, a Membrane-bound Bacitracin Sensor and DNA-binding Protein fromEnterococcus faecalis

2008 ◽  
Vol 283 (13) ◽  
pp. 8591-8600 ◽  
Author(s):  
Jonathan C. Gauntlett ◽  
Susanne Gebhard ◽  
Stefanie Keis ◽  
Janet M. Manson ◽  
Klaas M. Pos ◽  
...  
Keyword(s):  
Dna Binding ◽  
Molecular Analysis ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Membrane Bound

Mutations in bglY, the structural gene for the DNA-binding protein H1, affect expression of several Escherichia coli genes

Biochimie ◽  
1990 ◽  
Vol 72 (12) ◽  
pp. 889-891 ◽  
Author(s):  
P. Bertin ◽  
P. Lejeune ◽  
C. Laurent-Winter ◽  
A. Danchin
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Structural Gene ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Affect Expression

scholarly journals F sex factor of Escherichia coli K-12 codes for a single-stranded DNA binding protein.

1983 ◽  
Vol 80 (14) ◽  
pp. 4422-4426 ◽  
Author(s):  
A. L. Kolodkin ◽  
M. A. Capage ◽  
E. I. Golub ◽  
K. B. Low
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Single Stranded Dna ◽  
K 12
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