Loss of parvalbumin immunoreactivity defines selectively vulnerable thalamic reticular nucleus neurons following cardiac arrest in the rat

Neurons in the portion of the human thalamic reticular nucleus (RT) associated with the prefrontal cortex and mediodorsal thalamic nuclei were found to be selectively vulnerable to ischemic neuronal damage following relatively short (≤5-min) duration cardiac arrest. In contrast, selective sparing of these RT neurons occurred in cases with longer (>10-min) duration of arrest that was sufficient to produce extensive ischemic neuronal damage throughout the cerebral cortex and thalamic relay nuclei. The selective degeneration of RT neurons appears to require the sustained activity of corticothalamic or thalamocortical projections to the RT following the ischemic insult. Loss of RT neurons associated with the frontal cortex and mediodorsal thalamus may be the biological basis of some types of persisting cognitive deficits in attentional processing experienced by patients following cardiac arrest, open heart surgery, or other forms of brief global cerebral ischemia.

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Early Thalamic Injury After Resuscitation From Severe Asphyxial Cardiac Arrest in Developing Rats

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.737319 ◽

2021 ◽

Vol 9 ◽

Author(s):

Hoai T. Ton ◽

Katherine Raffensperger ◽

Michael Shoykhet

Keyword(s):

Cardiac Arrest ◽

Microglial Activation ◽

Neuronal Degeneration ◽

Ischemic Injury ◽

Selective Vulnerability ◽

Thalamic Reticular Nucleus ◽

Reticular Nucleus ◽

Severity Of Injury ◽

Hypoxic Ischemic Injury ◽

Asphyxial Cardiac Arrest

Children who survive cardiac arrest often develop debilitating sensorimotor and cognitive deficits. In animal models of cardiac arrest, delayed neuronal death in the hippocampal CA1 region has served as a fruitful paradigm for investigating mechanisms of injury and neuroprotection. Cardiac arrest in humans, however, is more prolonged than in most experimental models. Consequently, neurologic deficits in cardiac arrest survivors arise from injury not solely to CA1 but to multiple vulnerable brain structures. Here, we develop a rat model of prolonged pediatric asphyxial cardiac arrest and resuscitation, which better approximates arrest characteristics and injury severity in children. Using this model, we characterize features of microglial activation and neuronal degeneration in the thalamus 24 h after resuscitation from 11 and 12 min long cardiac arrest. In addition, we test the effect of mild hypothermia to 34°C for 8 h after 12.5 min of arrest. Microglial activation and neuronal degeneration are most prominent in the thalamic Reticular Nucleus (nRT). The severity of injury increases with increasing arrest duration, leading to frank loss of nRT neurons at longer arrest times. Hypothermia does not prevent nRT injury. Interestingly, injury occurs selectively in intermediate and posterior nRT segments while sparing the anterior segment. Since all nRT segments consist exclusively of GABA-ergic neurons, we asked if GABA-ergic neurons in general are more susceptible to hypoxic-ischemic injury. Surprisingly, cortical GABA-ergic neurons, like their counterparts in the anterior nRT segment, do not degenerate in this model. Hence, we propose that GABA-ergic identity alone is not sufficient to explain selective vulnerability of intermediate and posterior nRT neurons to hypoxic-ischemic injury after cardiac arrest and resuscitation. Our current findings align the animal model of pediatric cardiac arrest with human data and suggest novel mechanisms of selective vulnerability to hypoxic-ischemic injury among thalamic GABA-ergic neurons.

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Limited but Significant Protective Effect of Hypothermia on Ultra-Early-Type Ischemic Neuronal Injury in the Thalamus

Journal of Cerebral Blood Flow & Metabolism ◽

10.1097/00004647-199705000-00008 ◽

1997 ◽

Vol 17 (5) ◽

pp. 543-552 ◽

Cited By ~ 6

Author(s):

Kensuke Kawai ◽

Hitoshi Nakayama ◽

Akira Tamura

Keyword(s):

Cardiac Arrest ◽

Protective Effect ◽

Morphological Changes ◽

Thalamic Reticular Nucleus ◽

Reticular Nucleus ◽

Early Type ◽

Density Analysis ◽

Normothermic Group ◽

Ischemic Neuronal Injury ◽

Hypothermic Group

We investigated the protective effect of hypothermia on ultra-early-type ischemic injury in the thalamic reticular nucleus of the rat. Cerebral ischemia was produced by 5 min of cardiac arrest followed by resuscitation. Rectal and cranial temperature during and after cardiac arrest was maintained at 37–38°C in the normothermic group and at 32–33°C in the hypothermic group. In the postischemic hypothermic group, temperature was maintained at 32–33°C starting 15 min after normothermic ischemia. Histological damage was evaluated quantitatively. While after 5 min of recirculation there was no difference in morphological changes in terms of neuronal halo formation, intraischemic hypothermia reduced the severity of the degenerative changes represented by vacuolated or dark neurons by 15 min. Postischemic hypothermia failed to show any evidence of protection by 30 min. The protective effect of intraischemic hypothermia remained significant when evaluated at 14 days after ischemia by volumetry of the lesion and neuronal density analysis, whereas postischemic hypothermia had no clear protective effect. These results suggest that the protective effect of intraischemic hypothermia applies to neurons susceptible to ultra-early-type injury, but the effect of postischemic hypothermia is limited because normothermic ischemia results in extensive degeneration in these neurons by 15 min.

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Loss of parvalbumin immunoreactivity defines selectively vulnerable thalamic reticular nucleus neurons following cardiac arrest in the rat

Acta Neuropathologica ◽

10.1007/s004010050245 ◽

1995 ◽

Vol 89 (3) ◽

pp. 262-269

Author(s):

Kensuke Kawai ◽

T. S. Nowak Jr ◽

Igor Klatzo

Keyword(s):

Cardiac Arrest ◽

Thalamic Reticular Nucleus ◽

Reticular Nucleus

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Dorsal thalamus modulated by thalamic reticular nucleus

Protocol Exchange ◽

10.1038/nprot.2009.213 ◽

2009 ◽

Author(s):

Xiong-Jie Yu ◽

Shigang He ◽

Jufang He

Keyword(s):

Thalamic Reticular Nucleus ◽

Reticular Nucleus ◽

Dorsal Thalamus

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Thalamic Reticular Nucleus in Caiman crocodilus: Immunohistochemical Staining

Brain Behavior and Evolution ◽

10.1159/000496327 ◽

2018 ◽

Vol 92 (3-4) ◽

pp. 142-166 ◽

Cited By ~ 1

Author(s):

Michael B. Pritz

Keyword(s):

Glutamic Acid ◽

Glutamic Acid Decarboxylase ◽

Immunohistochemical Staining ◽

Medial Forebrain Bundle ◽

Transverse Plane ◽

Thalamic Reticular Nucleus ◽

Reticular Nucleus ◽

Acid Decarboxylase ◽

Caiman Crocodilus ◽

Monoclonal Mouse

The thalamic reticular nucleus in reptiles, Caiman crocodilus, shares a number of morphological similarities with its counterpart in mammals. In view of the immunohistochemical properties of this nucleus in mammals and the more recently identified complexity of this neuronal aggregate in Caiman, this nucleus was investigated using a number of antibodies. These results were compared with findings described for other amniotes. The following antibodies gave consistent and reproducible results: polyclonal sheep anti-parvalbumin (PV), monoclonal mouse anti-PV, and polyclonal sheep anti-glutamic acid decarboxylase (GAD). In the transverse plane, this nucleus is divided into two. In each part, a compact group of cells sits on top of the fibers of the forebrain bundle with scattered cells among these fibers. In the lateral forebrain bundle, this neuronal aggregate is represented by the dorsal peduncular nucleus and the perireticular nucleus while, in the medial forebrain bundle, these parts are the interstitial nucleus and the scattered cells in this fiber tract. The results of this study are the following. First, the thalamic reticular nucleus of Caiman contains GAD(+) and PV(+) neurons, which is similar to what has been described in other amniotes. Second, the morphology and distribution of many GAD(+) and PV(+) neurons in the dorsal peduncular and perireticular nuclei are similar and suggest that these neurons colocalize these markers. Third, neurons in the interstitial nucleus and in the medial forebrain bundle are GAD(+) and PV(+). At the caudal pole of the thalamic reticular nucleus, PV immunoreactive cells predominated and avoided the central portion of this nucleus where GAD(+) cells were preferentially located. However, GAD(+) cells were sparse when compared with PV(+) cells. This immunohistochemically different area in the caudal pole is considered to be an area separate from the thalamic reticular nucleus.

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The therapeutic mechanism of epilepsy seizures in different target areas: Research on a theoretical model

Technology and Health Care ◽

10.3233/thc-218043 ◽

2021 ◽

Vol 29 ◽

pp. 455-461

Author(s):

Bing Hu ◽

Zhizhi Wang ◽

Minbo Xu ◽

Luyao Zhu ◽

Dingjiang Wang

Keyword(s):

Pyramidal Neurons ◽

Control Mechanism ◽

Difficult Problem ◽

Calculation Model ◽

Thalamic Reticular Nucleus ◽

Reticular Nucleus ◽

Theoretical Support ◽

Therapeutic Mechanism ◽

Optimal Target ◽

Selection Of

BACKGROUND: The selection of optimal target areas in the surgical treatment of epilepsy is always a difficult problem in medicine. OBJECTIVE: We employed a theoretical calculation model to explore the control mechanism of seizures by an external voltage stimulus acting in different nerve nuclei. METHODS: Theoretical analysis and numerical simulation were combined. RESULTS: The globus pallidus, excitatory pyramidal neurons, striatal D1 neurons, thalamic reticular nucleus and specific relay nuclei were selected, we analyzed that the electrical stimulation has different effects in these target areas. CONCLUSIONS: The data selected were reasonable in study, the results may give a theoretical support for similar studies in clinical.

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Synaptic properties of the feedback connections from the thalamic reticular nucleus to the dorsal lateral geniculate nucleus

Journal of Neurophysiology ◽

10.1152/jn.00757.2019 ◽

2020 ◽

Vol 124 (2) ◽

pp. 404-417 ◽

Cited By ~ 1

Author(s):

Peter W. Campbell ◽

Gubbi Govindaiah ◽

Sean P. Masterson ◽

Martha E. Bickford ◽

William Guido

Keyword(s):

Lateral Geniculate Nucleus ◽

Dorsal Lateral Geniculate Nucleus ◽

Thalamic Reticular Nucleus ◽

Reticular Nucleus ◽

Lateral Geniculate ◽

Dependent Form ◽

Dorsal Lateral Geniculate ◽

Feedback Connections ◽

Spike Firing ◽

Stimulation Of

The thalamic reticular nucleus (TRN) modulates thalamocortical transmission through inhibition. In mouse, TRN terminals in the dorsal lateral geniculate nucleus (dLGN) form synapses with relay neurons but not interneurons. Stimulation of TRN terminals in dLGN leads to a frequency-dependent form of inhibition, with higher rates of stimulation leading to a greater suppression of spike firing. Thus, TRN inhibition appears more dynamic than previously recognized, having a graded rather than an all-or-none impact on thalamocortical transmission.

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