Alterations to the microtubular cytoskeleton and increased disorder of chromosome alignment in spontaneously ovulated mouse oocytes aged in vivo: an immunofluorescence study

Chromosoma ◽  
1986 ◽  
Vol 94 (5) ◽  
pp. 337-345 ◽  
Author(s):  
U. Eichenlaub-Ritter ◽  
A. C. Chandley ◽  
R. G. Gosden
Keyword(s):  
Immunofluorescence Study ◽  
Mouse Oocytes ◽  
Microtubular Cytoskeleton ◽  
Chromosome Alignment

Triphenyltin chloride induces spindle microtubule depolymerisation and inhibits meiotic maturation in mouse oocytes

2014 ◽  
Vol 26 (8) ◽  
pp. 1084 ◽  
Author(s):  
Yu-Ting Shen ◽  
Yue-Qiang Song ◽  
Xiao-Qin He ◽  
Fei Zhang ◽  
Xin Huang ◽  
...  
Keyword(s):  
Polar Body ◽  
Meiotic Maturation ◽  
Dependent Manner ◽  
Germinal Vesicle Breakdown ◽  
Mouse Oocytes ◽  
First Polar Body ◽  
Triphenyltin Chloride ◽  
Dose Dependent

Meiosis produces haploid gametes for sexual reproduction. Triphenyltin chloride (TPTCL) is a highly bioaccumulated and toxic environmental oestrogen; however, its effect on oocyte meiosis remains unknown. We examined the effect of TPTCL on mouse oocyte meiotic maturation in vitro and in vivo. In vitro, TPTCL inhibited germinal vesicle breakdown (GVBD) and first polar body extrusion (PBE) in a dose-dependent manner. The spindle microtubules completely disassembled and the chromosomes condensed after oocytes were exposed to 5 or 10 μg mL–1 TPTCL. γ-Tubulin protein was abnormally localised near chromosomes rather than on the spindle poles. In vivo, mice received TPTCL by oral gavage for 10 days. The general condition of the mice deteriorated and the ovary coefficient was reduced (P < 0.05). The number of secondary and mature ovarian follicles was significantly reduced by 10 mg kg–1 TPTCL (P < 0.05). GVBD decreased in a non-significant, dose-dependent manner (P > 0.05). PBE was inhibited with 10 mg kg–1 TPTCL (P < 0.05). The spindles of in vitro and in vivo metaphase II oocytes were disassembled with 10 mg kg–1 TPTCL. These results suggest that TPTCL seriously affects meiotic maturation by disturbing cell-cycle progression, disturbing the microtubule cytoskeleton and inhibiting follicle development in mouse oocytes.

scholarly journals Meiotic spindle morphogenesis in in vivo and in vitro matured mouse oocytes: insights into the relationship between nuclear and cytoplasmic quality

2004 ◽  
Vol 19 (12) ◽  
pp. 2889-2899 ◽  
Author(s):  
Alexandra Sanfins ◽  
Carlos E. Plancha ◽  
Eric W. Overstrom ◽  
David F. Albertini
Keyword(s):  
Meiotic Spindle ◽  
Mouse Oocytes ◽  
The Relationship

The in vivo and in vitro effects of clomiphene citrate on ovulation, fertilization, and development of cultured mouse oocytes

1983 ◽  
Vol 147 (6) ◽  
pp. 633-639 ◽  
Author(s):  
N. Laufer ◽  
B.M. Pratt ◽  
A.H. DeCherney ◽  
F. Naftolin ◽  
M. Merino ◽  
...  
Keyword(s):  
Clomiphene Citrate ◽  
Mouse Oocytes ◽  
In Vitro Effects

Injury effects of ginkgolide B on maturation of mouse oocytes, fertilization, and fetal development in vitro and in vivo

2009 ◽  
Vol 188 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Nion-Heng Shiao ◽  
Wen-Hsiung Chan
Keyword(s):  
Fetal Development ◽  
Ginkgolide B ◽  
Mouse Oocytes ◽  
Development In Vitro

scholarly journals Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes

2013 ◽  
Vol 58 (26) ◽  
pp. 3248-3255
Author(s):  
Ning Tian ◽  
Lu Zhang ◽  
DanYu Lü ◽  
JingGao Zheng ◽  
WanYun Ma ◽  
...  
Keyword(s):  
Dna Methyltransferases ◽  
Expression Levels ◽  
Chromosome Architecture ◽  
Mouse Oocytes ◽  
Maternal Aging

scholarly journals In vivo antisense oligodeoxynucleotide mapping reveals masked regulatory elements in an mRNA dormant in mouse oocytes.

1997 ◽  
Vol 17 (4) ◽  
pp. 1759-1767 ◽  
Author(s):  
A Stutz ◽  
J Huarte ◽  
P Gubler ◽  
B Conne ◽  
D Belin ◽  
...  
Keyword(s):  
Translational Control ◽  
Intact Cell ◽  
Regulatory Elements ◽  
Tissue Type ◽  
Meiotic Maturation ◽  
Antisense Oligodeoxynucleotide ◽  
Control Element ◽  
Rnase Protection ◽  
Mouse Oocytes

In mouse oocytes, tissue-type plasminogen activator (tPA) mRNA is under translational control. The newly transcribed mRNA undergoes deadenylation and translational silencing in growing oocytes, while readenylation and translation occur during meiotic maturation. To localize regulatory elements controlling tPA mRNA expression, we identified regions of the endogenous transcript protected from hybridization with injected antisense oligodeoxynucleotides. Most of the targeted sequences in either the 5' untranslated region (5'UTR), coding region, or 3'UTR were accessible to hybridization, as revealed by inhibition of tPA synthesis and by RNase protection. Two protected regions were identified in the 3'UTR of tPA mRNA in primary oocytes: the adenylation control element (ACE) and the AAUAAA polyadenylation signal. These sequences were previously shown to be involved in the translational control of injected reporter transcripts. During the first hour of meiotic maturation, part of the ACE and the AAUAAA hexanucleotide became accessible to hybridization, suggesting a partial unmasking of the 3'UTR of this mRNA before it becomes translationally competent. Our results demonstrate that in vivo antisense oligodeoxynucleotide mapping can reveal the dynamics of regulatory features of a native mRNA in the context of the intact cell. They suggest that specific regions in the 3'UTR of tPA mRNA function as cis-acting masking determinants involved in the silencing of tPA mRNA in primary oocytes.

Comparison of spindle and chromosome configuration in in vitro- and in vivo-matured mouse oocytes after vitrification

2008 ◽  
Vol 90 (4) ◽  
pp. 1424-1432 ◽  
Author(s):  
Jack Y.J. Huang ◽  
Hai Ying Chen ◽  
Joseph You Sup Park ◽  
Seang Lin Tan ◽  
Ri-Cheng Chian
Keyword(s):  
Chromosome Configuration ◽  
Mouse Oocytes

scholarly journals Full-term development of enucleated mouse oocytes fused with embryonic stem cells from different cell lines

Reproduction ◽  
2001 ◽  
pp. 729-733 ◽  
Author(s):  
T Amano ◽  
Y Kato ◽  
Y Tsunoda
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Lines ◽  
Formation Rate ◽  
Embryonic Stem ◽  
Clear Correlation ◽  
Developmental Potential ◽  
Mouse Oocytes

The developmental potential of enucleated mouse oocytes receiving embryonic stem cells from ten lines with either the same or different genetic backgrounds using the cell fusion method was examined in vitro and in vivo. The development of nuclear-transferred oocytes into blastocysts was high (34-88%). However, there was no clear correlation between development into blastocysts after nuclear transfer and the chimaera formation rate of embryonic stem cells. The development into live young was low (1-3%) in all cell lines and 14 of 19 young died shortly after birth. Most of the live young had morphological abnormalities. Of the five remaining mice, two died at days 23 and 30 after birth, but the other three mice are still active at days 359 (mouse 1) and 338 (mice 4 and 5) after birth, with normal fertility. However, the reasons for the abnormalities and postnatal death of embryonic stem cell-derived mice are unknown.

scholarly journals Strategic Targeting of CENPE-PDL1 axis in NSCLC

2020 ◽  
Author(s):  
Jinyan Liang ◽  
Chen Tian ◽  
Qifan Yang ◽  
Feifei Gu ◽  
Guoliang Pi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Treg Cell ◽  
Antitumor Immunity ◽  
Negative Effects ◽  
Cell Responses ◽  
Immune Mediated ◽  
Chromosome Alignment ◽  
Anti Tumor Activity

Abstract Background Increasing evidence suggests that centromere-associated protein E (CENP-E) is expressed during mitosis and plays a key role in incorrect chromosome alignment. Therefore, CENP-E may represent a druggable target for several solid tumors. Methods Here, we evaluated the ability of the CENPE inhibitor GSK923295 to up-regulate PDL1 and induce immune responses to tumor-associated CD8 T cell and regulatory T (Treg) cell.Results Our study found that a CENP-E inhibitor exhibited anti-tumor activity by directly suppressing the proliferation of lung cancer cells and upregulating the expression of PD-L1. Inhibition of CENP-E suppressed antitumor immunity by attenuating the response of activated CD8+ T cells and augmenting Tregs in vitro and in vivo. Mechanistically, CENP-E bound to the TTP promoter to regulate its transcription, and inhibition of CENP-E stabilized the mRNA of PD-L1 via TTP targeting of the 3’UTR. Inhibition or knockdown of CENP-E combined with an anti-PD-L1 antibody rescued the impaired antitumor CD8+ T/Treg cell response and improved the antitumor effect in lung cancer. Surprisingly , further analysis found that CENP-E was only related to the poor prognosis of lung cancer. Conclusions All of these results suggest that a CENP-E inhibitor will exert anti-tumor effects and upregulate PD-L1-induced impairment of anti-tumor CD8+/Treg cell responses in lung cancer. However, elevated PDL1 levels provide a possible strategy for combination immunotherapy, and this combination of immunotherapeutic strategies may offset the negative effects of a CENP-E inhibitor on its immune-mediated antitumor ability in lung cancer treatment.

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