An examination of factors affecting the instability of Saccharomyces cerevisiae glucan synthetase in cell free extracts

1984 ◽  
Vol 137 (3) ◽  
pp. 209-214 ◽  
Author(s):  
F. Leal ◽  
J. Ruiz-Herrera ◽  
J. R. Villanueva ◽  
G. Larriba
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Factors Affecting ◽  
Glucan Synthetase

Analysis of Key Factors Affecting Ethanol Production by Saccharomyces cerevisiae IFST-072011

2012 ◽  
Vol 11 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Md. Fakruddin ◽  
Md. Abdul Quay ◽  
Monzur Morshed Ah ◽  
Naiyyum Choudhury
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Key Factors ◽  
Factors Affecting

scholarly journals Analysis of Transcriptional Activation at a Distance in Saccharomyces cerevisiae

2007 ◽  
Vol 27 (15) ◽  
pp. 5575-5586 ◽  
Author(s):  
Krista C. Dobi ◽  
Fred Winston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcriptional Activation ◽  
Target Gene ◽  
Core Promoter ◽  
Striking Difference ◽  
Base Pairs ◽  
Long Distance ◽  
Factors Affecting ◽  
Promoter Specificity

ABSTRACT Most fundamental aspects of transcription are conserved among eukaryotes. One striking difference between yeast Saccharomyces cerevisiae and metazoans, however, is the distance over which transcriptional activation occurs. In S. cerevisiae, upstream activation sequences (UASs) are generally located within a few hundred base pairs of a target gene, while in Drosophila and mammals, enhancers are often several kilobases away. To study the potential for long-distance activation in S. cerevisiae, we constructed and analyzed reporters in which the UAS-TATA distance varied. Our results show that UASs lose the ability to activate normal transcription as the UAS-TATA distance increases. Surprisingly, transcription does initiate, but proximally to the UAS, regardless of its location. To identify factors affecting long-distance activation, we screened for mutants allowing activation of a reporter when the UAS-TATA distance is 799 bp. These screens identified four loci, SIN4, SPT2, SPT10, and HTA1-HTB1, with sin4 mutations being the strongest. Our results strongly suggest that long-distance activation in S. cerevisiae is normally limited by Sin4 and other factors and that this constraint plays a role in ensuring UAS-core promoter specificity in the compact S. cerevisiae genome.

scholarly journals Front Cover: Characterization of Terminators in Saccharomyces cerevisiae and an Exploration of Factors Affecting Their Strength (ChemBioChem 24/2017)

ChemBioChem ◽  
2017 ◽  
Vol 18 (24) ◽  
pp. 2366-2366
Author(s):  
Linna Wei ◽  
Zhaoxia Wang ◽  
Genlin Zhang ◽  
Bangce Ye
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Front Cover ◽  
Factors Affecting

Factors affecting the mitotic stability of high-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae

Yeast ◽  
1996 ◽  
Vol 12 (5) ◽  
pp. 467-477 ◽  
Author(s):  
T. S. Lopes ◽  
I. J. De Wijs ◽  
S. I. Steenhauer ◽  
J. Verbakel ◽  
R. J. Planta
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ribosomal Dna ◽  
Copy Number ◽  
High Copy Number ◽  
Mitotic Stability ◽  
Factors Affecting

Factors affecting the consumption of 2,3-butanedione by Saccharomyces cerevisiae

1994 ◽  
Vol 18 (3) ◽  
pp. 132-134 ◽  
Author(s):  
Clara María Olguín-Castillo ◽  
E. Galindo ◽  
M. Salvador-Figueroa
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Factors Affecting

scholarly journals Development of a Saccharomyces cerevisiae Strain with Enhanced Resistance to Phenolic Fermentation Inhibitors in Lignocellulose Hydrolysates by Heterologous Expression of Laccase

2001 ◽  
Vol 67 (3) ◽  
pp. 1163-1170 ◽  
Author(s):  
Simona Larsson ◽  
Pierre Cassland ◽  
Leif J. Jönsson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Mass ◽  
Raw Materials ◽  
White Rot ◽  
White Rot Fungus ◽  
Fermentation Inhibitors ◽  
Factors Affecting ◽  
Ethanol Formation ◽  
Active Laccase ◽  
Lignocellulose Hydrolysates

ABSTRACT To improve production of fuel ethanol from renewable raw materials, laccase from the white rot fungus Trametes versicolor was expressed under control of the PGK1 promoter inSaccharomyces cerevisiae to increase its resistance to phenolic inhibitors in lignocellulose hydrolysates. It was found that the laccase activity could be enhanced twofold by simultaneous overexpression of the homologous t-SNARE Sso2p. The factors affecting the level of active laccase obtained, besides the cultivation temperature, included pH and aeration. Laccase-expressing and Sso2p-overexpressing S. cerevisiae was cultivated in the presence of coniferyl aldehyde to examine resistance to lignocellulose-derived phenolic fermentation inhibitors. The laccase-producing transformant had the ability to convert coniferyl aldehyde at a faster rate than a control transformant not expressing laccase, which enabled faster growth and ethanol formation. The laccase-producing transformant was also able to ferment a dilute acid spruce hydrolysate at a faster rate than the control transformant. A decrease in the content of low-molecular-mass aromatic compounds, accompanied by an increase in the content of high-molecular-mass compounds, was observed during fermentation with the laccase-expressing strain, illustrating that laccase was active even at the very low levels of oxygen supplied. Our results demonstrate the importance of phenolic compounds as fermentation inhibitors and the advantage of using laccase-expressing yeast strains for producing ethanol from lignocellulose.

Factors affecting chemical-based purification of DNA from Saccharomyces cerevisiae

Yeast ◽  
2011 ◽  
Vol 29 (2) ◽  
pp. 73-80 ◽  
Author(s):  
Christopher K. Lee ◽  
Naoko Araki ◽  
Drew S. Sowersby ◽  
L. Kevin Lewis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Factors Affecting

Properties of ?-glucan synthetase from Saccharomyces cerevisiae

1978 ◽  
Vol 44 (3-4) ◽  
pp. 329-339 ◽  
Author(s):  
E. L�pez-Romero ◽  
J. Ruiz-Herrera
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucan Synthetase
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