Evaluation of a passive microcapsule agglutination test for the screening of human leptospirosis

1993 ◽  
Vol 9 (1) ◽  
pp. 92-96
Author(s):  
B. Cacciapuoti ◽  
L. Ciceroni ◽  
Y. Arimitsu ◽  
T. Sato ◽  
M. Seki
Keyword(s):  
Agglutination Test ◽  
Human Leptospirosis

scholarly journals Epidemiological situation of human leptospirosis in Brazil and challenges in its diagnosis with a focus on molecular approaches

Biotemas ◽  
2021 ◽  
Vol 34 (4) ◽  
pp. 1-13
Author(s):  
Andressa Penedo de Paiva Estrella ◽  
Amanda Silva Hecktheuer ◽  
Fabienne Antunes Ferreira ◽  
Ricardo Ruiz Mazzon
Keyword(s):  
Diagnostic Test ◽  
Agglutination Test ◽  
Diagnostic Methods ◽  
Polymorphism Analysis ◽  
General View ◽  
Routine Test ◽  
Human Leptospirosis ◽  
Molecular Approaches ◽  
Worldwide Distribution ◽  
Causative Agents

Leptospira interrogans is one of the causative agents of human leptospirosis, a zoonotic disease with worldwide distribution. Nowadays, this zoonosis is considered one of the biggest in terms of morbidity and mortality (even considering Dengue, the major arbovirosis affecting humans), having in Brazil 3,800 human cases per year. Currently, difficulties imposed by the absence of a rapid, sensitive diagnostic test that can be used as a routine test for the detection of leptospirosis lead to misdiagnosis and underreported cases. The gold standard diagnostic test for leptospirosis is the microscopic agglutination test (MAT), which presents difficulties in execution and interpretation. Therefore, this review proposes a general view of the epidemiologic situation of the disease in Brazil, in addition to the current contributions in the literature for the development of new diagnostic methods. Amongst them, the gene sequences polymorphism analysis, which presents potential for phylogenetic and populational analysis and genotyping of Leptospira spp.

scholarly journals SEROLOGICAL AND MOLECULAR SURVEY OF Leptospira spp. AMONG CART HORSES FROM AN ENDEMIC AREA OF HUMAN LEPTOSPIROSIS IN CURITIBA, SOUTHERN BRAZIL

2014 ◽  
Vol 56 (6) ◽  
pp. 473-476 ◽  
Author(s):  
Mariane Angélica Finger ◽  
Ivan Roque de Barros Filho ◽  
Christian Leutenegger ◽  
Marko Estrada ◽  
Leila Sabrina Ullmann ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Endemic Area ◽  
Agglutination Test ◽  
Southern Brazil ◽  
Chain Reaction ◽  
Human Leptospirosis ◽  
Leptospira Spp ◽  
Polymerase Chain ◽  
Positive Correlations

Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.

scholarly journals Macroscopic Agglutination Test for Rapid Diagnosis of Human Leptospirosis

1998 ◽  
Vol 36 (11) ◽  
pp. 3138-3142 ◽  
Author(s):  
Angela P. Brandão ◽  
Eide D. Camargo ◽  
Emilson D. da Silva ◽  
Marcos V. Silva ◽  
Rui V. Abrão
Keyword(s):  
Blood Donors ◽  
Agglutination Test ◽  
Immunoglobulin M ◽  
Screening Tests ◽  
Enzyme Linked Immunosorbent Assay ◽  
Initial Screening ◽  
Early Screening ◽  
Human Leptospirosis ◽  
Igm Elisa

A commercially available slide agglutination test (SAT) for the diagnosis of human leptospirosis was evaluated by comparing it to an immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) and to the microscopic agglutination test (MAT). For all 108 patients, leptospirosis was diagnosed on the basis of a fourfold or greater increase in titer by MAT (seroconversion), and all but 1 of 245 controls were MAT negative (titers, <1:100). Both SAT and the IgM ELISA failed to detect one case of infection (sensitivity, 99%). Only 3 of 145 blood donors and none of the 100 patients with other illnesses were SAT positive (specificity, 99%). The overall results were similar for the three tests; however, SAT and ELISA were statistically more sensitive as initial screening tests. For 22% of the patients, the diagnosis of leptospirosis was made earlier by SAT than by MAT. SAT detected 27 (44%) of 62 MAT-negative patients with the first serum sample. ELISA and SAT had very similar results. Follow-up of patients for 1 year after the onset of symptoms showed a decreasing rate of positivity by SAT from the third month on. The rate of positivity by ELISA decreased more slowly, to about 67% by the end of the study. By MAT all patients were persistently reactive. SAT and ELISA seem to be convenient methods for the rapid and early screening for leptospirosis and could replace the less sensitive MAT. ELISA gives less subjective results than SAT and provides information on IgM kinetics, but it can be performed only by the more sophisticated laboratories. SAT is inexpensive, can be performed more quickly and more easily than ELISA, and could be used by the less well equipped laboratories.

scholarly journals Comparison between latex and microscopic agglutination test for detection of human leptospiral antibodies

2016 ◽  
Vol 10 (3) ◽  
pp. 219
Author(s):  
Ehsanollah Sakhaee ◽  
Mehdi Golchin ◽  
Zahra Davoodian
Keyword(s):  
Febrile Illness ◽  
High Sensitivity ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Basic Method ◽  
Microscopic Agglutination Test ◽  
Leptospira Interrogans ◽  
Acute Febrile Illness ◽  
Specific Antibodies ◽  
Human Leptospirosis

Leptospirosis, notably Weil’s syndrome, is an often severe, acute febrile illness caused by microorganisms of the genus <em>Leptospira</em>. The microscopic agglutination test (MAT) is the basic method for the sero-diagnosis of leptospirosis, as the test has a high sensitivity and can be used for classification, but has some disadvantages. Therefore, we have explored latex agglutination test (LAT) for use as a practical and rapid for sero-diagnosis of human leptospirosis and compared the applicability of commercial tests MAT and LAT in the detection of specific antibodies against <em>Leptospira interrogans</em> in human.

scholarly journals Microscopic agglutination test: Variables that affect the time of serological confirmation of human leptospirosis cases

2020 ◽  
Vol 52 (4) ◽  
pp. 278-282
Author(s):  
Exequiel Scialfa ◽  
Mariana Rivero ◽  
Sergio Moreno ◽  
Marcela Ortiz ◽  
Fabricio Bongiorno
Keyword(s):  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Human Leptospirosis

scholarly journals Evaluation of an indirect hemagglutination test for the diagnosis of human leptospirosis

1975 ◽  
Vol 2 (3) ◽  
pp. 218-221
Author(s):  
C R Sulzer ◽  
J W Glosser ◽  
F Rogers ◽  
W L Jones ◽  
M Frix
Keyword(s):  
Agglutination Test ◽  
Serological Diagnosis ◽  
Alcohol Extract ◽  
Slide Agglutination ◽  
Human Leptospirosis ◽  
Hemagglutination Test ◽  
Slide Agglutination Test ◽  
Indirect Hemagglutination ◽  
Slide Test ◽  
Indirect Hemagglutination Test

A presumptive hemagglutination test for the serological diagnosis of leptospirosis in humans is described. The antigen was prepared from a soluble alcohol extract of an andamana strain sorbed to human O-negative erythrocytes and preserved by pyruvic aldehyde fixation. In this study, the overall sensitivity of the hemagglutination test was 92% in contrast to 69% for the presumptive slide agglutination test. The specificity was 95% for the hemagglutination test in comparison with 83% for the slide test.

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