Genetic, ontogenetic, and tissue-specific variation of dipeptidases in Drosophila melanogaster

1982 ◽  
Vol 20 (5-6) ◽  
pp. 407-424 ◽  
Author(s):  
C. C. Laurie-Ahlberg
Keyword(s):  
Drosophila Melanogaster ◽  
Tissue Specific ◽  
Specific Variation

Region-specific variation of gene expression in the human epididymis as revealed by in situ hybridization with tissue-specific cDNAs

1993 ◽  
Vol 34 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Nora Krull ◽  
Richard Ivell ◽  
Caroline Osterhoff ◽  
Christiane Kirchhoff
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Tissue Specific ◽  
Human Epididymis ◽  
Specific Variation

scholarly journals Sex and tissue‐specific evolution of developmental plasticity in Drosophila melanogaster

2020 ◽  
Author(s):  
Didem P. Sarikaya ◽  
Katherine Rickelton ◽  
Julie M. Cridland ◽  
Ryan Hatmaker ◽  
Hayley K. Sheehy ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Developmental Plasticity ◽  
Tissue Specific

A Modified TurboID Approach Identifies Tissue-Specific Centriolar Components In C. elegans

2021 ◽  
Author(s):  
Elisabeth Holzer ◽  
Cornelia Rumpf-Kienzl ◽  
Sebastian Falk ◽  
Alexander Dammermann
Keyword(s):  
Protein Interactions ◽  
Affinity Purification ◽  
Experimental Models ◽  
Protein Protein Interactions ◽  
Tissue Specific ◽  
C Elegans ◽  
Early Embryos ◽  
Somatic Tissues ◽  
Specific Variation ◽  
Labeling Method

Proximity-dependent labeling approaches such as BioID have been a great boon to studies of protein-protein interactions in the context of cytoskeletal structures such as centrosomes which are poorly amenable to traditional biochemical approaches like immunoprecipitation and tandem affinity purification. Yet, these methods have so far not been applied extensively to invertebrate experimental models such as C. elegans given the long labeling times required for the original promiscuous biotin ligase variant BirA*. Here, we show that the recently developed variant TurboID successfully probes the interactomes of both stably associated (SPD-5) and dynamically localized (PLK-1) centrosomal components. We further develop an indirect proximity labeling method employing a GFP nanobody- TurboID fusion, which allows the identification of protein interactors in a tissue-specific manner in the context of the whole animal. Critically, this approach utilizes available endogenous GFP fusions, avoiding the need to generate multiple additional strains for each target protein and the potential complications associated with overexpressing the protein from transgenes. Using this method, we identify homologs of two highly conserved centriolar components, Cep97 and Bld10/Cep135, which are present in various somatic tissues of the worm. Surprisingly, neither protein is expressed in early embryos, likely explaining why these proteins have escaped attention until now. Our work expands the experimental repertoire for C. elegans and opens the door for further studies of tissue-specific variation in centrosome architecture.

scholarly journals Three-dimensional organization of Drosophila melanogaster interphase nuclei. I. Tissue-specific aspects of polytene nuclear architecture.

1987 ◽  
Vol 104 (6) ◽  
pp. 1455-1470 ◽  
Author(s):  
M Hochstrasser ◽  
J W Sedat
Keyword(s):  
Drosophila Melanogaster ◽  
Three Dimensional ◽  
Nuclear Architecture ◽  
Close Packing ◽  
Prothoracic Gland ◽  
Cell Type ◽  
Interphase Chromosome ◽  
Tissue Specific ◽  
Spatial Domains ◽  
Four Levels

Interphase chromosome organization in four different Drosophila melanogaster tissues, covering three to four levels of polyteny, has been analyzed. The results are based primarily on three-dimensional reconstructions from unfixed tissues using a computer-based data collection and modeling system. A characteristic organization of chromosomes in each cell type is observed, independent of polyteny, with some packing motifs common to several or all tissues and others tissue-specific. All chromosomes display a right-handed coiling chirality, despite large differences in size and degree of coiling. Conversely, in each cell type, the heterochromatic centromeric regions have a unique structure, tendency to associate, and intranuclear location. The organization of condensed nucleolar chromatin is also tissue-specific. The tightly coiled prothoracic gland chromosomes are arrayed in a similar fashion to the much larger salivary gland chromosomes described previously, having polarized orientations, nonintertwined spatial domains, and close packing of the arms of each autosome, whereas hindgut and especially the unusually straight midgut chromosomes display striking departures from these regularities. Surprisingly, gut chromosomes often appear to be broken in the centric heterochromatin. Severe deformations of midgut nuclei observed during gut contractions in living larvae may account for their unusual properties. Finally, morphometric measurements of chromosome and nuclear dimensions provide insights into chromosome growth and substructure and also suggest an unexpected parallel with diploid chromatin organization.

scholarly journals Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18853 ◽  
Author(s):  
Ying Chen ◽  
Hongzheng Dai ◽  
Sidi Chen ◽  
Luoying Zhang ◽  
Manyuan Long
Keyword(s):  
Drosophila Melanogaster ◽  
Male Courtship ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Characterization of the genetic architecture underlying eye size variation within Drosophila melanogaster and Drosophila simulans

2019 ◽  
Author(s):  
Pedro Gaspar ◽  
Saad Arif ◽  
Lauren Sumner-Rooney ◽  
Maike Kittelmann ◽  
Andrew J. Bodey ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Natural Variation ◽  
Size Variation ◽  
Eye Size ◽  
Specific Variation ◽  
Gene Regulatory ◽  
Morphological Differences ◽  
Genomic Regions ◽  
Insect Eye

AbstractThe compound eyes of insects exhibit striking variation in size, reflecting adaptation to different lifestyles and habitats. However, the genetic and developmental bases of variation in insect eye size is poorly understood, which limits our understanding of how these important morphological differences evolve. To address this, we further explored natural variation in eye size within and between four species of the Drosophila melanogaster species subgroup. We found extensive variation in eye size among these species, and flies with larger eyes generally had a shorter inter-ocular distance and vice versa. We then carried out quantitative trait loci (QTL) mapping of intra-specific variation in eye size and inter-ocular distance in both D. melanogaster and D. simulans. This revealed that different genomic regions underlie variation in eye size and inter-ocular distance in both species, which we corroborated by introgression mapping in D. simulans. This suggests that although there is a trade-off between eye size and inter-ocular distance, variation in these two traits is likely to be caused by different genes and so can be genetically decoupled. Finally, although we detected QTL for intra-specific variation in eye size at similar positions in D. melanogaster and D. simulans, we observed differences in eye fate commitment between strains of these two species. This indicates that different developmental mechanisms and therefore, most likely, different genes contribute to eye size variation in these species. Taken together with the results of previous studies, our findings suggest that the gene regulatory network that specifies eye size has evolved at multiple genetic nodes to give rise to natural variation in this trait within and among species.

Tissue-Specific variation in the length of the 5? untranslated region of the ?a-inhibin mRNA in sheep

1995 ◽  
Vol 40 (1) ◽  
pp. 1-8 ◽  
Author(s):  
J. S. Fleming ◽  
S. M. Galloway ◽  
R. J. Crawford ◽  
D. J. Tisdall ◽  
P. J. Greenwood
Keyword(s):  
Untranslated Region ◽  
Tissue Specific ◽  
Specific Variation

A novel, tissue-specific integrin subunit, beta nu, expressed in the midgut of Drosophila melanogaster

Development ◽  
1993 ◽  
Vol 118 (3) ◽  
pp. 845-858 ◽  
Author(s):  
G.H. Yee ◽  
R.O. Hynes
Keyword(s):  
Drosophila Melanogaster ◽  
Matrix Proteins ◽  
Beta Subunits ◽  
Integrin Subunit ◽  
Integrin Receptor ◽  
Tissue Specific ◽  
Surface Receptors ◽  
Polymerase Chain ◽  
Maximal Expression

The integrins are a family of cell surface receptors for extracellular matrix proteins and counter-receptors on other cells. We have used the polymerase chain reaction to identify a novel integrin receptor beta subunit in Drosophila melanogaster. The deduced amino acid sequence of this subunit, which we have termed beta v (beta-neu), indicates that it has several unusual properties. The beta v subunit is roughly 33% identical with each of the previously sequenced vertebrate and Drosophila beta subunits and is lacking four of the 56 cysteine residues characteristic of most members of this protein family. The expression of the beta v gene is strikingly restricted. It is temporally regulated, with maximal expression occurring at 12–15 hours of embryonic development. In situ hybridization analyses and antibody localization on whole-mount embryos reveal that beta v expression is tissue-specific and confined to the developing midgut endoderm and its precursors during embryogenesis. Tissue specificity of expression is maintained through later stages of development as beta v transcripts are found exclusively in the larval midgut. Within this structure, beta v transcripts are especially concentrated in the cells of the midgut imaginal islands which give rise to the adult midgut.

Tissue-specific transcription of the neuronal gene Lim3 affects Drosophila melanogaster lifespan and locomotion

2017 ◽  
Vol 18 (5) ◽  
pp. 739-757 ◽  
Author(s):  
Olga Y. Rybina ◽  
Svetlana V. Sarantseva ◽  
Ekaterina R. Veselkina ◽  
Olga I. Bolschakova ◽  
Alexander V. Symonenko ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Tissue Specific ◽  
Neuronal Gene
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