In vivo identification of muscarinic receptors on rat colonic epithelial cells binding of [3H]-quinuclidinyl benzilate

Abstract Background: In our study, has_circ_102209 was the most upregulated gene in colorectal cancer (CRC) tissues according to circRNA array data. The levels of hsa_circ_102209 in CRC specimens and cells, as well as its effects on CRC cells were investigated. Methods: The expression of hsa_circ_102209 in CRC and paired non-cancerous samples, human CRC and normal colonic epithelial cells were examined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cells with hsa_circ_102209 knockdown were established using lentiviral vectors . Cell proliferative ability was evaluated using CCK-8 assay; cell migration and invasion were assessed by wound healing and Transwell assay. Cell cycle arrest and apoptosis were determined; apoptosis and EMT markers were examined using RT-qPCR and western blotting. Tumour development and levels of associated proteins were determined in hsa_circ_102209 knockdown mice. Results: Our results revealed that expression of hsa_circ_102209 was remarkably increased in CRC tissues, where the levels of miR-761 were notably reduced (p<0.05). Additionally, the levels of hsa_circ_102209 was associated with tumor stage and occurrence of liver metastasis in CRC patients, and the expression of hsa_circ_102209 and miR-761 were negatively correlated (p<0.05). Moreover, hsa_circ_102209 was upregulated in CRC cell s compared with normal colonic epithelial cells. Knockdown of hsa_circ_102209 notably inhibited the proliferation, migration, invasion and EMT of CRC cell s (p<0.05), whereas enhanced cell cycle arrest at G0/G1 phase and apoptosis (p<0.05). Furthermore, miR-761/ Ras and Rab interactor 1 ( RIN1) axis was the putative target of hsa_circ_102209 in CRC and involved in hsa_circ_102209 -modulated growth and metastasis in CRC cell s (p<0.05). Knockdown of hsa_circ_102209 also remarkably suppressed tumor growth in vivo (p<0.05). Conclusions: our data revealed that the expression of hsa_circ_102209 was elevated in CRC samples and cells. Furthermore, hsa_circ_102209 could promote the progression of CRC through miR-761/RIN1 axis. More importantly, hsa_circ_102209 /miR-761/RIN1 signaling may be a novel therapeutic target for the treatment of CRC patients .

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Circular RNA hsa_circ_102209 promotes the growth and metastasis of colorectal cancer through miR-761-mediated Ras and Rab interactor 1 signaling

10.21203/rs.2.24638/v2 ◽

2020 ◽

Author(s):

CHI LI ◽

Hong Zhou

Keyword(s):

Colorectal Cancer ◽

Cell Cycle ◽

Epithelial Cells ◽

Cell Cycle Arrest ◽

Quantitative Polymerase Chain Reaction ◽

Circular Rna ◽

Migration And Invasion ◽

Colonic Epithelial Cells ◽

Cycle Arrest

Abstract Background: The levels of hsa_circ_102209 in colorectal cancer (CRC) specimens and cells, as well as its effects on CRC cells were investigated. Methods: The expression of hsa_circ_102209 in CRC and paired non-cancerous samples, human CRC and normal colonic epithelial cells were examined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cells with hsa_circ_102209 knockdown were established using lentiviral vectors. Cell proliferative ability was evaluated using CCK-8 assay; cell migration and invasion were assessed by wound healing and Transwell assay. Cell cycle arrest and apoptosis were determined; apoptosis and EMT markers were examined using RT-qPCR and western blotting. Tumour development and levels of associated proteins were determined in hsa_circ_102209 knockdown mice. Results: Our results revealed that expression of hsa_circ_102209 was remarkably increased in CRC tissues, where the levels of miR-761 were notably reduced (p<0.05). Additionally, the levels of hsa_circ_102209 was associated with histology grade and occurrence of liver metastasis in CRC patients, and the expression of hsa_circ_102209 and miR-761 were negatively correlated (p<0.05). Moreover, hsa_circ_102209 was upregulated in CRC cells compared with normal colonic epithelial cells. Knockdown of hsa_circ_102209 notably inhibited the proliferation, migration, invasion and EMT of CRC cells (p<0.05), whereas enhanced cell cycle arrest at G0/G1 phase and apoptosis (p<0.05). Furthermore, miR-761/Ras and Rab interactor 1 (RIN1) axis was the putative target of hsa_circ_102209 in CRC and involved in hsa_circ_102209-modulated growth and metastasis in CRC cells (p<0.05). Knockdown of hsa_circ_102209 also remarkably suppressed tumor growth in vivo (p<0.05). Conclusions: our data revealed that the expression of hsa_circ_102209 was elevated in CRC samples and cells. Furthermore, hsa_circ_102209 could promote the progression of CRC through miR-761/RIN1 axis. More importantly, hsa_circ_102209/miR-761/RIN1 signaling may be a novel therapeutic target for the treatment of CRC patients.

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Faculty Opinions recommendation of Boosting apoptotic cell clearance by colonic epithelial cells attenuates inflammation in vivo.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726260385.793519401 ◽

2016 ◽

Author(s):

Philippe Saas ◽

Sylvain Perruche ◽

Etienne Daguindau

Keyword(s):

Epithelial Cells ◽

Apoptotic Cell ◽

Colonic Epithelial Cells ◽

Apoptotic Cell Clearance ◽

Cell Clearance

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Antigen targeting to MHC class II‐enriched late endosomes in colonic epithelial cells: trafficking of luminal antigens studied in vivo in Crohn's colitis patients

The FASEB Journal ◽

10.1096/fj.05-4807fje ◽

2005 ◽

Vol 20 (2) ◽

pp. 359-361 ◽

Cited By ~ 21

Author(s):

Jürgen Büning ◽

Gheorghe Hundorfean ◽

Martina Schmitz ◽

Klaus‐Peter Zimmer ◽

Stephan Strobel ◽

...

Keyword(s):

Epithelial Cells ◽

Mhc Class Ii ◽

Class Ii ◽

Colonic Epithelial Cells ◽

Crohn’S Colitis ◽

Crohn's Colitis ◽

Late Endosomes

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Butyrate acts as a survival factor for colonic epithelial cells: Further fuel for the in vivo versus in vitro debate

Gastroenterology ◽

10.1053/gast.1997.v112.agast971036 ◽

1997 ◽

Vol 112 (3) ◽

pp. 1036-1040 ◽

Cited By ~ 106

Author(s):

A Hague ◽

B Singh ◽

C Paraskeva

Keyword(s):

Epithelial Cells ◽

Colonic Epithelial Cells ◽

Survival Factor

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The VSL#3 probiotic formula induces mucin gene expression and secretion in colonic epithelial cells

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00265.2006 ◽

2007 ◽

Vol 292 (1) ◽

pp. G315-G322 ◽

Cited By ~ 243

Author(s):

C. Caballero-Franco ◽

K. Keller ◽

C. De Simone ◽

K. Chadee

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Cultured Cells ◽

In Vivo Studies ◽

Lactobacillus Species ◽

Mucin Secretion ◽

Colonic Epithelial Cells ◽

Dnase Treatment

Several studies have stressed the importance of the microbiota in the maintenance of the gastrointestinal epithelium. Administration of probiotic bacteria, supplements composed of microbiota constituents, was previously shown to diminish symptoms in patients suffering from inflammatory bowel diseases. This raises the possibility that probiotics may play an active role in enhancing the intestinal barrier at the mucosal surface. In this study, we investigated whether the clinically tested VSL#3 probiotic formula and/or its secreted components can augment the protective mucus layer in vivo and in vitro. For in vivo studies, Wistar rats were orally administered the probiotic mixture VSL#3 on a daily basis for seven days. After treatment, basal luminal mucin content increased by 60%. In addition, we exposed isolated rat colonic loops to the VSL#3 probiotic formula, which significantly stimulated colonic mucin (MUC) secretion and MUC2 gene expression; however, MUC1 and MUC3 gene expression were only slightly elevated. The effect of the VSL#3 mucin secretagogue was also tested in vitro by use of LS 174T colonic epithelial cells. In contrast to the animal studies, cultured cells incubated with VSL#3 bacteria did not exhibit increased mucin secretion. However, the bacterial secreted products contained in the conditioned media stimulated a remarkable mucin secretion effect. Among the three bacterial groups ( Lactobacilli, Bifidobacteria, and Streptococci) contained in VSL#3, the Lactobacillus species were the strongest potentiator of mucin secretion in vitro. A preliminary characterization of the putative mucin secretagogue suggested that it was a heat-resistant soluble compound, which is not sensitive to protease and DNase treatment. These findings contribute to a better understanding of the complex and beneficial interaction between colonic epithelial cells and intestinal bacteria.

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In vivo induction of prostasin mRNA in colonic epithelial cells by dietary sodium depletion and aldosterone infusion in rats

Journal of Gastroenterology ◽

10.1007/s00535-004-1425-7 ◽

2004 ◽

Vol 39 (10) ◽

pp. 940-947 ◽

Cited By ~ 8

Author(s):

Kouhei Fukushima ◽

Hiroo Naito ◽

Yuji Funayama ◽

Hitoshi Yonezawa ◽

Sho Haneda ◽

...

Keyword(s):

Epithelial Cells ◽

Dietary Sodium ◽

Sodium Depletion ◽

Colonic Epithelial Cells ◽

In Vivo Induction

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Leptin modulates the expression of secreted and membrane-associated mucins in colonic epithelial cells by targeting PKC, PI3K, and MAPK pathways

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00091.2007 ◽

2007 ◽

Vol 293 (1) ◽

pp. G365-G373 ◽

Cited By ~ 61

Author(s):

Mahmoud El Homsi ◽

Robert Ducroc ◽

Jean Claustre ◽

Gérard Jourdan ◽

Arieh Gertler ◽

...

Keyword(s):

Epithelial Cells ◽

Mrna Level ◽

Intestinal Barrier Function ◽

Rat Colon ◽

Mrna Levels ◽

Colonic Epithelial Cells ◽

Mucin Production ◽

Leptin Receptors ◽

Mucin Expression

Mucins play an essential role in the protection and repair of gastrointestinal mucosa. We recently showed that luminal leptin strongly stimulated mucin secretion in vivo in rat colon. In the present study, we challenged the hypothesis that leptin may act directly on goblet cells to induce mucin expression in rat and human intestinal mucin-producing cells (DHE and HT29-MTX). The endoluminal effect of leptin was also studied in vivo in rat perfused colon model. The presence of leptin receptors was demonstrated in the two cell lines by Western blot and RT-PCR. In rat DHE cells, leptin (0.01–10 nmol/l, 60 min) dose dependently increased the secretion of mucins (210 ± 3% of controls) and the expression of Muc2, Muc3, and Muc4 (twofold basal level) but not of Muc1 and Muc5AC. Luminal perfusion of leptin (60 min, 0.1–100 nmol/l) in rat colon also increased the mRNA level of Muc2, Muc3, and Muc4 but not of Muc1. In human HT29-MTX cells, leptin (0.01–10 nmol/l, 60 min) dose dependently enhanced MUC2, MUC5AC, and MUC4 mRNA levels. These effects were prevented by pretreatment of cells with the leptin mutein L39A/D40A/F41A, which acts as a receptor antagonist. Finally, pathway inhibition experiments suggest that leptin increased mucin expression by activating PKC-, phosphatidyl inositol 3-kinase-, and MAPK-dependent pathways but not the JAK/STAT pathway. In conclusion, leptin may contribute significantly to membrane-associated and secreted mucin production via a direct stimulation of colonic epithelial cells and the activation of leptin receptors. These data are consistent with a role for leptin in regulation of the intestinal barrier function.

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Butyrate suppresses colonic inflammation through HDAC1-dependent Fas upregulation and Fas-mediated apoptosis of T cells

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00543.2011 ◽

2012 ◽

Vol 302 (12) ◽

pp. G1405-G1415 ◽

Cited By ~ 133

Author(s):

Mary A. Zimmerman ◽

Nagendra Singh ◽

Pamela M. Martin ◽

Muthusamy Thangaraju ◽

Vadivel Ganapathy ◽

...

Keyword(s):

Colorectal Cancer ◽

T Cells ◽

T Cell ◽

Epithelial Cells ◽

Genome Wide Association Studies ◽

Colonic Epithelial Cells ◽

Colonic Inflammation ◽

Altered Expression ◽

Ifn Γ

Butyrate, an intestinal microbiota metabolite of dietary fiber, has been shown to exhibit protective effects toward inflammatory diseases such as ulcerative colitis (UC) and inflammation-mediated colorectal cancer. Recent studies have shown that chronic IFN-γ signaling plays an essential role in inflammation-mediated colorectal cancer development in vivo, whereas genome-wide association studies have linked human UC risk loci to IFNG, the gene that encodes IFN-γ. However, the molecular mechanisms underlying the butyrate-IFN-γ-colonic inflammation axis are not well defined. Here we showed that colonic mucosa from patients with UC exhibit increased signal transducer and activator of transcription 1 (STAT1) activation, and this STAT1 hyperactivation is correlated with increased T cell infiltration. Butyrate treatment-induced apoptosis of wild-type T cells but not Fas-deficient (Fas lpr) or FasL-deficient (Fas gld) T cells, revealing a potential role of Fas-mediated apoptosis of T cells as a mechanism of butyrate function. Histone deacetylase 1 (HDAC1) was found to bind to the Fas promoter in T cells, and butyrate inhibits HDAC1 activity to induce Fas promoter hyperacetylation and Fas upregulation in T cells. Knocking down gpr109a or slc5a8, the genes that encode for receptor and transporter of butyrate, respectively, resulted in altered expression of genes related to multiple inflammatory signaling pathways, including inducible nitric oxide synthase (iNOS), in mouse colonic epithelial cells in vivo. Butyrate effectively inhibited IFN-γ-induced STAT1 activation, resulting in inhibition of iNOS upregulation in human colon epithelial and carcinoma cells in vitro. Our data thus suggest that butyrate delivers a double-hit: induction of T cell apoptosis to eliminate the source of inflammation and suppression of IFN-γ-mediated inflammation in colonic epithelial cells, to suppress colonic inflammation.

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