Immunohistochemical localization of extravasated serum albumin in the hippocampus of human subjects with partial and generalized epilepsies and epileptiform convulsions

Serial plasma free fatty acid (FFA), cholesterol, and triglyceride levels were measured in 5 normal human subjects and in 11 hypoalbuminemic patients after infusion of norepinephrine with and without prior infusion of 50 g of serum albumin. In the hypoalbuminemic patients the peak response in FFA level to norepinephrine was diminished; after albumin infusion the peak rise of FFA level to norepinephrine was enhanced in both the normal and hypoalbuminemic individuals. Alterations in serum triglyceride and cholesterol concentrations that followed albumin infusion could be accounted for by the alteration in plasma volume that also occurred. The diminished FFA response in hypoalbuminemic patients was interpreted as indicating that norepinephrine-induced fat mobilization in man is dependent on the concentration of serum albumin, and that the defect in fat mobilization in hypoalbuminemia may be, in part, related to the alterations in cholesterol and triglyceride metabolism that are characteristic of hypoalbuminemia. Submitted on April 20, 1962

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Immunohistochemical localization of fibronectin in human and rat uterine cervices.

Journal of Histochemistry & Cytochemistry ◽

10.1177/30.5.7042816 ◽

1982 ◽

Vol 30 (5) ◽

pp. 413-417 ◽

Cited By ~ 6

Author(s):

T J Leppi ◽

L A Repesh ◽

L T Furcht ◽

P J Bartzen ◽

G E Holt

Keyword(s):

Extracellular Matrix ◽

Connective Tissue ◽

Basement Membrane ◽

Human Subjects ◽

Immunohistochemical Localization ◽

Paraffin Sections ◽

Cervical Stroma ◽

Stromal Collagen ◽

Collagenous Fibers ◽

Uniform Deposition

Paraffin sections of 95% ethanol-fixed uterine cervices from adult, cycling rats and from human patients undergoing elective hysterectomy were immunostained by the peroxidase-antiperoxidase technique after exposure to antisera against fibronectin. Light microscopic study of uterine cervical stroma from both species showed an irregular distribution of fibronectin between compactly arranged collagenous fibers and a more uniform deposition around scattered bundles of smooth muscle fibers. In loosely arranged areas of stromal collagen, fibronectin was found in a filamentous, occasionally punctate, distribution. There was moderate to heavy staining with immunoperoxidase for fibronectin in the regions of the basement membrane underlying cervical epithelia and in the connective tissue stroma immediately subjacent to the basement membrane. Fibronectin was also present in the walls of most blood vessels and in the connective tissue investments of peripheral nerve fascicles. These findings suggest that fibronectin may be another component of extracellular matrix in the uterine cervical wall of non-gravid human subjects and rats in addition to collagen and glycosaminoglycans.

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COMPARATIVE STUDIES ON THE TURNOVER OF SERUM ALBUMIN IN NORMAL HUMAN SUBJECTS 1

Journal of Clinical Investigation ◽

10.1172/jci103370 ◽

1956 ◽

Vol 35 (10) ◽

pp. 1161-1172 ◽

Cited By ~ 36

Author(s):

Sheldon Margen ◽

Harold Tarver

Keyword(s):

Serum Albumin ◽

Comparative Studies ◽

Human Subjects ◽

Normal Human

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131I-Labeled Colloidal Human Serum Albumin in the Study of Reticuloendothelial System Function. III. Phagocytosis and Catabolism Compared in Normal, Leukemic, and Immunosuppressed Human Subjects

The Journal of Infectious Diseases ◽

10.1093/infdis/123.5.465 ◽

1971 ◽

Vol 123 (5) ◽

pp. 465-469 ◽

Cited By ~ 3

Author(s):

D. L. Palmer ◽

D. Rifkind ◽

D. W. Brown

Keyword(s):

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Human Subjects ◽

Reticuloendothelial System ◽

System Function

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Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128122 ◽

1987 ◽

Vol 45 ◽

pp. 762-763

Author(s):

G. D. Gagne ◽

M. F. Miller

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Artificial Substrate ◽

Chemical Fixation ◽

As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

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Elevated level of circulatory sTLT1 induces inflammation through SYK/MEK/ERK signalling in coronary artery disease

Clinical Science ◽

10.1042/cs20190999 ◽

2019 ◽

Vol 133 (22) ◽

pp. 2283-2299

Author(s):

Apabrita Ayan Das ◽

Devasmita Chakravarty ◽

Debmalya Bhunia ◽

Surajit Ghosh ◽

Prakash C. Mandal ◽

...

Keyword(s):

Risk Factors ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Chronic Inflammation ◽

Ex Vivo ◽

Human Subjects ◽

Critical Role ◽

Atherosclerotic Cardiovascular Disease ◽

Tnf Α ◽

Artery Disease

Abstract The role of inflammation in all phases of atherosclerotic process is well established and soluble TREM-like transcript 1 (sTLT1) is reported to be associated with chronic inflammation. Yet, no information is available about the involvement of sTLT1 in atherosclerotic cardiovascular disease. Present study was undertaken to determine the pathophysiological significance of sTLT1 in atherosclerosis by employing an observational study on human subjects (n=117) followed by experiments in human macrophages and atherosclerotic apolipoprotein E (apoE)−/− mice. Plasma level of sTLT1 was found to be significantly (P<0.05) higher in clinical (2342 ± 184 pg/ml) and subclinical cases (1773 ± 118 pg/ml) than healthy controls (461 ± 57 pg/ml). Moreover, statistical analyses further indicated that sTLT1 was not only associated with common risk factors for Coronary Artery Disease (CAD) in both clinical and subclinical groups but also strongly correlated with disease severity. Ex vivo studies on macrophages showed that sTLT1 interacts with Fcɣ receptor I (FcɣRI) to activate spleen tyrosine kinase (SYK)-mediated downstream MAP kinase signalling cascade to activate nuclear factor-κ B (NF-kB). Activation of NF-kB induces secretion of tumour necrosis factor-α (TNF-α) from macrophage cells that plays pivotal role in governing the persistence of chronic inflammation. Atherosclerotic apoE−/− mice also showed high levels of sTLT1 and TNF-α in nearly occluded aortic stage indicating the contribution of sTLT1 in inflammation. Our results clearly demonstrate that sTLT1 is clinically related to the risk factors of CAD. We also showed that binding of sTLT1 with macrophage membrane receptor, FcɣR1 initiates inflammatory signals in macrophages suggesting its critical role in thrombus development and atherosclerosis.

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