Use of on-line gas analysis to monitor recombinant mammalian cell cultures

1994 ◽  
Vol 14 (3) ◽  
pp. 167-175 ◽  
Author(s):  
George Lovrecz ◽  
Peter Gray
Keyword(s):  
Mammalian Cell ◽  
Cell Cultures ◽  
Gas Analysis ◽  
Mammalian Cell Cultures ◽  
On Line

scholarly journals Applications of off-gas mass spectrometry in fed-batch mammalian cell culture

2019 ◽  
Vol 43 (3) ◽  
pp. 483-493 ◽  
Author(s):  
Hai-Yuan Goh ◽  
Michael Sulu ◽  
Haneen Alosert ◽  
Graham L. Lewis ◽  
Graham D. Josland ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Cell Cultures ◽  
Mammalian Cell Culture ◽  
Lactate Production ◽  
Gas Analysis ◽  
Fed Batch ◽  
Batch Mode ◽  
Phase Average ◽  
Mammalian Cell Cultures

Abstract Off-gas analysis using a magnetic sector mass spectrometer was performed in mammalian cell cultures in the fed-batch mode at the 5 L bench and 50 L pilot scales. Factors affecting the MS gas traces were identified during the duration of the fed-batch cultures. Correlation between viable cell concentration (VCC) and oxygen concentration of the inlet gas into the bioreactor (O2-in) resulted in R2 ≈ 0.9; O2-in could be used as a proxy for VCC. Oxygen mass transfer (kLa) was also quantified throughout the culture period with antifoam addition at different time points which is shown to lower the kLa. Real-time specific oxygen consumption rate (qO2) of 2–20 pmol/cell/day throughout the bioreactor runs were within the range of values reported in literature for mammalian cell cultures. We also report, to our knowledge, the first instance of a distinct correlation between respiration quotient (RQ) and the metabolic state of the cell culture with regard to lactate production phase (average RQ > 1) and consumption phase (average RQ < 1).

On-Line Monitoring of Glutamine and Ammonium in Mammalian-Cell Cultures

1997 ◽  
pp. 429-434
Author(s):  
Eva Balslev Jorgensen ◽  
Antoni Casablancas ◽  
òGloria González ◽  
Francesc Gòdia
Keyword(s):  
Mammalian Cell ◽  
Cell Cultures ◽  
Mammalian Cell Cultures ◽  
On Line

Monitoring the Cell Size Distribution of Mammalian Cell Cultures Using On-Line Capacitance Measurements

2010 ◽  
pp. 853-859 ◽  
Author(s):  
Sven Ansorge ◽  
Olivier Henry ◽  
Marc Aucoin ◽  
Robert Voyer ◽  
John P. Carvell ◽  
...  
Keyword(s):  
Size Distribution ◽  
Cell Size ◽  
Mammalian Cell ◽  
Cell Cultures ◽  
Cell Size Distribution ◽  
Capacitance Measurements ◽  
Mammalian Cell Cultures ◽  
On Line

scholarly journals On-Line Control of Glucose Concentration in High-Yielding Mammalian Cell Cultures Enabled Through Oxygen Transfer Rate Measurements

2018 ◽  
Vol 13 (4) ◽  
pp. 1700607 ◽  
Author(s):  
Stephen Goldrick ◽  
Kenneth Lee ◽  
Christopher Spencer ◽  
William Holmes ◽  
Marcel Kuiper ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Cell Cultures ◽  
Glucose Concentration ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Oxygen Transfer Rate ◽  
Mammalian Cell Cultures ◽  
On Line

On-line monitoring of cell mass in mammalian cell cultures by acoustic densitometry

1989 ◽  
Vol 33 (11) ◽  
pp. 1379-1384 ◽  
Author(s):  
D. G. Kilburn ◽  
P. Fitzpatrick ◽  
B. C. Blake-Coleman ◽  
D. J. Clarke ◽  
J. B. Griffiths
Keyword(s):  
Mammalian Cell ◽  
Cell Cultures ◽  
Cell Mass ◽  
Mammalian Cell Cultures ◽  
On Line

On-Line Monitoring for Consistent and Optimal Production of Biologicals from Mammalian Cell Cultures

1997 ◽  
pp. 205-214 ◽  
Author(s):  
S. S. Ozturk ◽  
J. Blackie ◽  
P. Wu ◽  
R. Taticek ◽  
K. Konstantinov ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Cell Cultures ◽  
Optimal Production ◽  
Mammalian Cell Cultures ◽  
On Line

Mammalian Cell Cultures on Micropatterned Surfaces of Weak-Acid, Polyelectrolyte Hyperbranched Thin Films on Gold

2001 ◽  
Vol 73 (7) ◽  
pp. 1560-1566 ◽  
Author(s):  
Mary Lee Amirpour ◽  
Pradyut Ghosh ◽  
William M. Lackowski ◽  
Richard M. Crooks ◽  
Michael V. Pishko
Keyword(s):  
Thin Films ◽  
Mammalian Cell ◽  
Cell Cultures ◽  
Weak Acid ◽  
Mammalian Cell Cultures
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