Daily rhythm of mitotic division of the esophageal epithelial cells of albino rats

1963 ◽  
Vol 54 (2) ◽  
pp. 893-895
Author(s):  
V. N. Dobrokhotov ◽  
A. G. Kurdyumova
1978 ◽  
Vol 67 (04) ◽  
pp. 265-275 ◽  
Author(s):  
Suresh Prasad ◽  
K. Chandrasekhar

SummaryA comparative study of Pulsatilla (a homœopathic medicine) and leutocyclin was undertaken in female albino rats showing regular oestrus cycles. Pulsatilla in the 30th and 200th potencies was administered orally, and leutocyclin was injected in doses of 0·125 mg and 1·25 mg. 0·25 ml/dose of 90 per cent. alcohol was used as the vehicle. Pulsatilla 30 and 200 and leutocyclin at a dose of 1·25 mg markedly reduced the weight of the ovary. The higher dose of leutocyclin (1·25 mg) and both the attenuations of Pulsatilla increased the process of atresia in ovarian follicles. But a simultaneous decrease in the number and diameters of the corpora lutea was observed.Administration of Pulsatilla and leutocyclin resulted in reduction in the weight of the uterus and the height of the endometrium and luminal epithelium. There was also a reduction in the height of epithelial cells in the thyroid. Both Pulsatilla and leutocyclin enhanced mitotic division in the uterine stroma.The results indicate that leutocyclin in doses of between 0·125 mg and 1·25 mg may be used to produce similar changes in the ovary, uterus and thyroid of the rat as Pulsatilla 30 and 200.


Author(s):  
W.T. Gunning ◽  
M.R. Marino ◽  
M.S. Babcock ◽  
G.D. Stoner

The role of calcium in modulating cellular replication and differentiation has been described for various cell types. In the present study, the effects of Ca++ on the growth and differentiation of cultured rat esophageal epithelial cells was investigated.Epithelial cells were isolated from esophagi taken from 8 week-old male CDF rats by the enzymatic dissociation method of Kaighn. The cells were cultured in PFMR-4 medium supplemented with 0.25 mg/ml dialyzed fetal bovine serum, 5 ng/ml epidermal growth factor, 10-6 M hydrocortisone 10-6 M phosphoethanolamine, 10-6 M ethanolamine, 5 pg/ml insulin, 5 ng/ml transferrin, 10 ng/ml cholera toxin and 50 ng/ml garamycin at 36.5°C in a humidified atmosphere of 3% CO2 in air. At weekly intervals, the cells were subcultured with a solution containing 1% polyvinylpyrrolidone, 0.01% EGTA, and 0.05% trypsin. After various passages, the replication rate of the cells in PFMR-4 medium containing from 10-6 M to 10-3 M Ca++ was determined using a clonal growth assay.


Author(s):  
Julia L. M. Dunn ◽  
Julie M. Caldwell ◽  
Adina Ballaban ◽  
Netali Ben-Baruch Morgenstern ◽  
Mark Rochman ◽  
...  

2020 ◽  
Vol 145 (2) ◽  
pp. AB45
Author(s):  
Vinay Goswamy ◽  
Paul Fichtinger ◽  
Elizabeth McKernan ◽  
Sameer Mathur

ASAIO Journal ◽  
1999 ◽  
Vol 45 (5) ◽  
pp. 502-508 ◽  
Author(s):  
HIROTAKA MIKI ◽  
NOBUTOSHI ANDO ◽  
SOJI OZAWA ◽  
MICHIO SATO ◽  
KOJI HAYASHI ◽  
...  

2021 ◽  
Vol 160 (6) ◽  
pp. S-429-S-430
Author(s):  
Olivia D. Franklin ◽  
Ann De La Forest ◽  
Roman Stavniichuk ◽  
Kirthi Pulakanti ◽  
Sridhar Rao ◽  
...  

ASAIO Journal ◽  
1993 ◽  
Vol 39 (3) ◽  
pp. M554-M557 ◽  
Author(s):  
Michio Sato ◽  
Nobutoshi Ando ◽  
Soji Ozawa ◽  
Atsushi Nagashima ◽  
Masaki Kitajima

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