Translacental effect of urethane on DNA synthesis in mouse embryonic lung tissue in organotypical culture

1978 ◽  
Vol 86 (4) ◽  
pp. 1314-1316 ◽  
Author(s):  
T. S. Kolesnichenko ◽  
N. B. Gusina
Keyword(s):  
Dna Synthesis ◽  
Lung Tissue ◽  
Embryonic Lung ◽  
Organotypical Culture

scholarly journals COLLAGEN BIOSYNTHESIS

1957 ◽  
Vol 3 (5) ◽  
pp. 685-695 ◽  
Author(s):  
J. Frederick Woessner ◽  
Bernard S. Gould
Keyword(s):  
Ascorbic Acid ◽  
Cell Growth ◽  
Lung Tissue ◽  
Appreciable Effect ◽  
Collagen Biosynthesis ◽  
Embryonic Lung ◽  
Marked Contrast ◽  
Collagen Formation ◽  
Quantitative Studies ◽  
Total Failure

Quantitative studies of collagen formation by chick embryonic lung tissue grown in media deficient in, or completely lacking, ascorbic acid have been carried out. Cell growth and collagen formation in such cultures can proceed almost normally in media lacking ascorbic acid. Ascorbic acid in combination with whole embryo extract, dialyzed media, or synthetic mixture number 703 was found to have no appreciable effect on cell growth or total collagen formation. This is in marked contrast to the almost total failure of collagen formation in scorbutic animals and suggests that for slow collagen biosynthesis as distinct from more prolific collagen-producing systems, ascorbic acid plays an indirect role.

Action of allogeneic serum from a pregnant pneumonectomized rat on embryonic lung tissue

1978 ◽  
Vol 86 (1) ◽  
pp. 931-935
Author(s):  
I. I. Orlova ◽  
N. G. Lisatova ◽  
S. D. Mikhal'chenko
Keyword(s):  
Lung Tissue ◽  
Embryonic Lung

PDGF-AA and its receptor influence early lung branching via an epithelial-mesenchymal interaction

Development ◽  
1995 ◽  
Vol 121 (8) ◽  
pp. 2559-2567 ◽  
Author(s):  
P. Souza ◽  
M. Kuliszewski ◽  
J. Wang ◽  
I. Tseu ◽  
A.K. Tanswell ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Critical Role ◽  
Polymerase Chain Reaction Analysis ◽  
Branching Morphogenesis ◽  
Inhibitory Effect ◽  
Embryonic Lung ◽  
Morphometric Analyses ◽  
Terminal Buds ◽  
Lung Branching

The biological role of platelet-derived growth factor (PDGF)-AA in lung morphogenesis was investigated by incubating embryonic lung explants with phosphorothioate antisense PDGF-A oligonucleotides, which decreased PDGF-AA but not PDGF-BB protein content. Antisense PDGF-A oligonucleotides inhibited DNA synthesis. This inhibitory effect of antisense PDGF-A was reversed by the addition of exogenous PDGF-AA but not PDGF-BB. Morphometric analyses of antisense-treated cultures showed a significant reduction in lung size. The number of terminal buds of the lung explants was significantly decreased by antisense PDGF-A oligonucleotides. PDGF-AA but not PDGF-BB attenuated the inhibitory effect of antisense PDGF-A on early lung branching. Sense PDGF-A had no effect on DNA synthesis and early lung branching. Reverse transcriptase-polymerase chain reaction analysis revealed PDGF-A mRNA expression in the epithelial component of the embryonic lung, while message for PDGF alpha-receptor was expressed in the mesenchyme. Incubation of explants with neutralizing PDGF-AA antibodies also reduced DNA synthesis and early branching morphogenesis. We conclude that PDGF-AA and its receptor represent an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.

The structure of long-surviving homografts of embryonic lung tissue in rats

1964 ◽  
Vol 2 (4) ◽  
pp. 576
Author(s):  
R. A. Willis ◽  
L. T. Hou
Keyword(s):  
Lung Tissue ◽  
Embryonic Lung

Keratinocyte growth factor and its receptor are involved in regulating early lung branching

Development ◽  
1996 ◽  
Vol 122 (10) ◽  
pp. 3107-3115 ◽  
Author(s):  
M. Post ◽  
P. Souza ◽  
J. Liu ◽  
I. Tseu ◽  
J. Wang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Dna Synthesis ◽  
Keratinocyte Growth Factor ◽  
Inhibitory Effect ◽  
Lung Epithelial Cells ◽  
Embryonic Lung ◽  
Tissue Interactions ◽  
Lung Epithelial ◽  
Lung Branching

Lung branching morphogenesis depends on mesenchymal-epithelial tissue interactions. Keratinocyte growth factor (KGF) has been implicated to be a regulator of these tissue interactions. In the present study, we investigated the role of KGF in early rat lung organogenesis. Reverse transcriptase-polymerase chain reaction analysis revealed KGF mRNA expression in the mesenchymal component of the 13-day embryonic lung, while message for KGF receptor (KGFR) was expressed in the epithelium, confirming the paracrine nature of KGF/KGFR axis. Antisense KGF oligonucleotides inhibited DNA synthesis of embryonic lung explants. This inhibitory effect of antisense KGF was partially reversed by the addition of exogenous KGF. Recombinant KGF was mitogenic for 13-day isolated embryonic lung epithelial cells. Medium conditioned by 13-day lung mesenchymal cells also stimulated DNA synthesis of 13-day embryonic lung epithelial cells. This stimulatory effect was partially abrogated by a neutralizing KGF antibody. The number of terminal buds of lung explants cultured in the presence of antisense KGF oligonucleotides was significantly reduced compared to control explants. Exogenous KGF partially abrogated the inhibitory effect of antisense KGF on early lung branching. Sense or scrambled KGF oligonucleotides had no inhibitory effect on lung growth and branching. Addition of neutralizing KGF antibodies to the explants also reduced the degree of branching, while non-immune IgG and neutralizing acidic FGF antibodies had no effect. Explants incubated with antisense oligonucleotides targeted to the initiation site of translation of both the splice variants of the fibroblast growth factor receptor-2 (FGFR2) gene, KGFR and bek, exhibited a similar reduction in lung branching as observed with antisense KGF oligonucleotides. Antisense KGFR-specific oligonucleotides dramatically inhibited lung branching, while exposure of explants to antisense bek-specific oligonucleotides resulted in reduced branching albeit to a lesser degree than that observed with antisense KGFR-specific oligonucleotides. Neither sense nor scrambled KGFR-specific oligonucleotides had any effect on early lung branching. These results suggest that the KGF/KGFR system has a critical role in early lung organogenesis.

Elastin biosynthesis in chick embryonic lung tissue. Comparison to chick aortic elastin

Biochemistry ◽  
1981 ◽  
Vol 20 (12) ◽  
pp. 3528-3535 ◽  
Author(s):  
Judith Ann Foster ◽  
Celeste B. Rich ◽  
Sharon Fletcher ◽  
Steven R. Karr ◽  
Marcia D. DeSa ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Embryonic Lung

Organ culture of human embryonic lung tissue

1975 ◽  
Vol 80 (2) ◽  
pp. 967-970
Author(s):  
T. S. Kolesnichenko ◽  
T. V. Nikonova
Keyword(s):  
Organ Culture ◽  
Lung Tissue ◽  
Embryonic Lung ◽  
Human Embryonic Lung

scholarly journals Engraftment and proliferation potential of embryonic lung tissue cells in irradiated mice with emphysema

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kazushige Shiraishi ◽  
Shigeyuki Shichino ◽  
Tatsuya Tsukui ◽  
Shinichi Hashimoto ◽  
Satoshi Ueha ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Embryonic Lung ◽  
Tissue Cells ◽  
Proliferation Potential

Effect of cigarette-smoke condensates on homografts of embryonic lung tissue in rats

1963 ◽  
Vol 86 (1) ◽  
pp. 199-207 ◽  
Author(s):  
Lee-Tsün Hou ◽  
R. A. Willis
Keyword(s):  
Cigarette Smoke ◽  
Lung Tissue ◽  
Embryonic Lung

Antisense oligodeoxynucleotides targeting PDGF-B mRNA inhibit cell proliferation during embryonic rat lung development

Development ◽  
1994 ◽  
Vol 120 (8) ◽  
pp. 2163-2173 ◽  
Author(s):  
P. Souza ◽  
L. Sedlackova ◽  
M. Kuliszewski ◽  
J. Wang ◽  
J. Liu ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Lung Development ◽  
Neutralizing Antibodies ◽  
Fetal Lung ◽  
Inhibitory Effect ◽  
Antisense Oligodeoxynucleotides ◽  
Dependent Manner ◽  
Rat Lung ◽  
Embryonic Lung ◽  
Concentration Dependent Manner

There is increasing evidence to suggest that platelet-derived growth factor (PDGF) or PDGF-like molecules play a role in fetal lung morphogenesis. Our previous studies demonstrated the presence of PDGF-AA and PDGF-BB homodimers in embryonic and fetal rat lung. To explore further the role for PDGF-BB in embryonic lung development, we conducted intervention studies using PDGF-B chain-specific antisense oligodeoxynucleotides in a simple embryonic rat lung explant system. Unmodified antisense PDGF-B oligodeoxynucleotides inhibited, in a concentration-dependent manner, DNA synthesis of embryonic lung. A maximal inhibition of 50% was observed. The inhibitory effect of antisense PDGF-B oligodeoxynucleotides on DNA synthesis was reversed by the addition of exogenous PDGF-BB but not PDGF-AA. Antisense treatment decreased PDGF-BB but not PDGF-AA protein content, as assessed by immunoblot analyses. Incubation of lung explants with PDGF-BB neutralizing antibodies also resulted in an inhibition of DNA synthesis. Morphometric analyses of antisense-treated cultures showed a significant reduction in lung size when compared to control cultures. The epithelial component of the embryonic lungs was specifically reduced, both in mass and DNA labelling index, by antisense treatment. The number of terminal buds of the lung explants was not significantly affected by antisense PDGF-B treatment. Scrambled PDGF-B oligodeoxynucleotides had no effect. These data suggest that PDGF-BB is involved in regulating growth, but not the degree of branching, of embryonic rat lung.

Sign in / Sign up

Export Citation Format

Share Document