A new dynamic model system for the study of capture reactions for diffusable compounds in cytochemistry. III. Influence of the matrix composition on the lead phosphate precipitation process in acid phosphatase cytochemistry

1979 ◽  
Vol 11 (2) ◽  
pp. 163-171 ◽  
Author(s):  
A. S. H. De Jong ◽  
T. J. Hak ◽  
P. Van Duijn ◽  
W. Th. Daems
Keyword(s):  
Acid Phosphatase ◽  
Dynamic Model ◽  
Model System ◽  
Matrix Composition ◽  
Precipitation Process ◽  
Lead Phosphate ◽  
The Matrix ◽  
Phosphatase Cytochemistry

scholarly journals A new dynamic model system for the study of capture reactions for diffusable compounds in cytochemistry. I. Description of the model with special attention to phosphate capture in acid phosphatase cytochemistry.

1978 ◽  
Vol 26 (5) ◽  
pp. 331-339 ◽  
Author(s):  
A S de Jong ◽  
T J Hak ◽  
P van Duijn ◽  
W T Daems
Keyword(s):  
Acid Phosphatase ◽  
Model System ◽  
Phosphate Concentration ◽  
Model Studies ◽  
System A ◽  
Phosphate Ions ◽  
The Matrix ◽  
Phosphatase Cytochemistry ◽  
Trapping Reactions ◽  
Bone Calcification

A model system is described for the investigation of the dynamics of precipitation processes in a matrix. In this system a solution containing the molecular species to be precipitated and the precipitating medium are pumped along opposite sides of a polyacrylamide film. The solutions flowing continuously along the film, interact and can form a precipitate inside the film. The applicability of the model was tested on the capture reaction for phosphate ions by the Gomori type medium for acid phosphatase. Precipitation of lead phosphate in the film occurred only at a phosphate concentration above a certain value. The dependence of this minimal phosphate concentration on various parameters was studied and the results were compared with values found in earlier model studies and calculations concerning phosphate concentrations that can be built up in lysosomes during the Gomori reaction. The system seems promising for obtaining fundamental data about other cytochemical enzyme trapping reactions as well as for the matrix facotrs involved in bone calcification and shell formation.

scholarly journals A NEW METHOD FOR THE INVESTIGATION OF THE CAPTURE REACTION IN PHOSPHATASE CYTOCHEMISTRY III. EFFECTS OF THE COMPOSITION OF THE INCUBATION MEDIUM ON THE TRAPPING OF PHOSPHATE IONS IN A MODEL SYSTEM

1974 ◽  
Vol 22 (2) ◽  
pp. 110-119 ◽  
Author(s):  
C. J. CORNELISSE ◽  
P. VAN DUIJN
Keyword(s):  
Model System ◽  
Chloride Ions ◽  
Trapping Efficiency ◽  
Lead Salt ◽  
Lead Phosphate ◽  
Enzyme Cytochemistry ◽  
Factors Affecting ◽  
Capture Reaction ◽  
Phosphate Ions ◽  
Phosphatase Cytochemistry

A model system developed for the study of diffusion problems in lead salt enzyme cytochemistry served as a basis for the experiments reported in the present paper. Phosphate leakage, which was investigated in polyacrylamide films during incubation in media containing lead, could be expressed by a graphical parameter related to the average displacement of the phosphate ions prior to the onset of lead phosphate precipitation in the films. This parameter can be used as a measure for the efficiency of the capture reaction in cytochemical reactions in tissue sections. The concentration of lead ions, and to a lesser extent that of the phosphate ions, was found to be critical for the degree of phosphate diffusion in this system. Addition of 0.3% of β-glycerophosphate to the lead medium gave increased phosphate diffusion, and trapping was markedly improved by added chloride ions. Raising of the molarity of the acetate buffer resulted in impaired trapping efficiency. Similar findings were made in a parallel study on the kinetics of lead phosphate precipitation in supersaturated solutions with a nephelometric method. The results of the present investigation indicate that the film model system is highly suitable for the study of factors affecting the rapid immobilization of diffusible products of cytochemical reactions.

Demonstration of Secondary Lysosomes in Bovine Megakaryocytes and Platelets Using Acid Phosphatase Cytochemistry with Cerium as a Trapping Agent

1990 ◽  
Vol 63 (01) ◽  
pp. 127-132 ◽  
Author(s):  
Michèle Ménard ◽  
Kenneth M Meyers ◽  
David J Prieur
Keyword(s):  
Acid Phosphatase ◽  
Electron Density ◽  
Golgi Complex ◽  
Initial Report ◽  
Cerium Phosphate ◽  
Virtual Absence ◽  
Phosphatase Cytochemistry ◽  
Secondary Lysosomes ◽  
Trapping Agent

SummaryThe ultrastructure of lysosomes from bovine megakaryocytes (MK) and platelets was characterized using acid phosphatase cytochemistry with beta-glycerophosphate as substrate and cerium as a trapping agent. The technique was easily reproducible; cerium-phosphate precipitates were uniform, readily visualized, and there was a virtual absence of nonspecific reaction product. Acid phosphatase was localized in the trans aspect of the Golgi complex and/or granules of less than 50 nm to 650 nm diameters in MK at all stages of maturation. Forty percent of the MK lysosomes contained inclusions of variable shapes, sizes and electron-density and were classified as secondary lysosomes. Twenty-four percent of the platelet sections contained acid phosphatase-positive granules. Fifty-four percent of these were secondary lysosomes. This is the initial report demonstrating secondary lysosomes in either resting MK or platelets using acid phosphatase cytochemistry. These findings suggest that MK and platelet lysosomes have an intracellular function in resting MK and platelets.

Precipitation of highly luminescent phases from PECVD Si suboxides

2004 ◽  
Vol 832 ◽  
Author(s):  
M. Perálvarez ◽  
M. López ◽  
B. Garrido ◽  
J.R. Morante ◽  
J. Barreto ◽  
...  
Keyword(s):  
Refractive Index ◽  
Phase Separation ◽  
Ion Implantation ◽  
Composition Profile ◽  
Matrix Composition ◽  
Light Sources ◽  
The Matrix ◽  
Detailed Composition ◽  
Preparation Route

ABSTRACTSi nanoclusters (Si-nc) embedded in SiO2 present outstanding luminescent emission in the visible and are the material of choice for the realization of efficient light sources integrated with Si technology. PECVD is an attractive preparation route but there is still the need to understand how Si excess and matrix composition affect the precipitation of Si-nc and their photoluminescence (PL) efficiency. The SiOx PECVD layers studied here have a Si excess up to 50% and a thickness between 50 and 100 nm. The phase separation, precipitation and growth of the Si-nc have been achieved by annealing at 1250 °C. For reference, the same study has been performed in Si-nc/SiO2 materials synthesized by ion implantation and annealing. Refractive index and thickness measured by ellipsometry show a densification of the layers after the H release during annealing. A detailed composition profile has been determined by XPS and FTIR analyses and shows almost complete phase separation except for the interfaces, where a depletion of Si-nc is found. EFTEM demonstrates that isolated Si-nc are formed for Si excess up to 25% while for higher Si excess a continuous Si phase is observed. The PL efficiency in PECVD samples is maximized for a Si excess of 17% which is the same Si excess than that for the most emitting implanted samples. No dependence of PL efficiency has been found on the presence of Nitrogen in the matrix (up to the 10%).

Slurry Wear Test of Long Kenaf Polyester Composite (LKPC) and Long Kenaf Polyester with Fiberglass Composite

2013 ◽  
Vol 393 ◽  
pp. 919-924
Author(s):  
Muhd Azimin Ab Ghani ◽  
A.F. Ahmad Kamal ◽  
Mohamad Ali Ahmad ◽  
Y.M.D. Taib ◽  
Z. Salleh ◽  
...  
Keyword(s):  
Sea Water ◽  
Wear Test ◽  
Matrix Composition ◽  
Slurry Erosion ◽  
Composite Surface ◽  
Wear And Friction ◽  
Fiberglass Composite ◽  
The Matrix ◽  
Polyester Composite ◽  
Erosion Test

This paper presents an experimental study on the influence of fiberglass woven in Long Kenaf Polyester Composite (LKPC). Wear and friction characteristics were examined using sea water and sand as a slurry. Wear test were carried out using Slurry Erosion Test Rig (TR-40). These tests were performed at room temperature with speed of 200 rpm for every 2 km interval. The results from the tests show that mass loss were depends on the matrix composition of the composite. Surface Roughness, Ra, was consistently reduced after each test in all cases.

Influence of WC Particle Size and Matrix Composition on the Behavior of WC-Co-Cr Coatings Sprayed by the HVOF Process

1997 ◽  
Author(s):  
J. Berget ◽  
E. Bardal ◽  
T. Rogne
Keyword(s):  
Particle Size ◽  
Wear Resistance ◽  
Corrosion Resistance ◽  
Chromium Content ◽  
Deposition Efficiency ◽  
Matrix Composition ◽  
Coating Properties ◽  
Hvof Process ◽  
Erosion Corrosion ◽  
The Matrix

Abstract WC-Co-Cr powders with different WC particle size have been sprayed by the HVOF process. At constant spraying conditions the powders give coatings of different quality. The deposition efficiency during spraying of powders containing large WC particles was found to be low compared to powders with finer WC grains. In addition the amounts of porosity and cracks were different. The coatings have been characterised by different methods. Erosion and erosion-corrosion tests showed that the WC particle size also influence the wear resistance of the coatings. Small WC particle size was found to be beneficial. Chemical composition of the matrix was also found to be decisive for the coating properties. An increase of the chromium content improved the erosion-corrosion resistance.

Sign in / Sign up

Export Citation Format

Share Document