Evaluation of the cobas bact automated system for susceptibility testing of enterobacteriaceae, pseudomonas aeruginosa, and enterococcus faecalis to azlocillin, mezlocillin, and ciprofloxacin compared to NCCLS and DIN standards

Infection ◽  
1988 ◽  
Vol 16 (1) ◽  
pp. 63-68 ◽  
Author(s):  
W. Heizmann ◽  
H. Werner ◽  
F. Heilmann
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Susceptibility Testing ◽  
Automated System

Multicenter clinical evaluation of VITEK® 2 meropenem-vaborbactam for susceptibility testing of Enterobacterales and Pseudomonas aeruginosa

2021 ◽  
Author(s):  
Hari P. Dwivedi ◽  
Simone Franklin ◽  
Sukantha Chandrasekaran ◽  
Omai Garner ◽  
Maria M. Traczewski ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Susceptibility Testing ◽  
Automated System ◽  
Individual Species ◽  
Method Performance ◽  
Overall Performance ◽  
Vitek 2 ◽  
Tract Infections ◽  
Alternate Solution ◽  
After Error

The carbapenem/beta-lactamase inhibitor (meropenem-vaborbactam; MEV) used to treat complicated urinary tract infections and pyelonephritis in adults was approved in 2017 by the U.S. Food and Drug Administration (FDA). We evaluated VITEK 2 MEV (bioMérieux, Durham, NC) compared to the reference broth microdilution (BMD) method. Of 449 Enterobacterales isolates analyzed per FDA/CLSI breakpoints, overall performance was 98.2% Essential Agreement (EA), 98.7% Category Agreement (CA), and 0% Very Major Errors (VME) or Major Errors (ME). For FDA intended for use 438 Enterobacterales isolates, performance was 98.2% EA, 98.6% CA, and 0% VME or ME. Evaluable EA was 81.0% but with only 42 on-scale evaluable results. Individual species demonstrated EA and CA rates ≥ 90% without any VME or ME. When evaluated using European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints, overall VITEK 2 MEV performance for Enterobacterales and Pseudomonas aeruginosa demonstrated 97.3% EA, 99.2% CA, 2.3% VME, and 0.6% ME (after error resolution: 97.3% EA, 99.4% CA, 2.2% VME, and 0.4% ME) compared to the reference BMD method. Performance for P. aeruginosa included 92.2% EA, 97.4% CA, 0% VME, and 3.0% ME (after error resolution: 92.2% EA, 98.7% CA, 0% VME, and 1.5% ME). Performance for Enterobacterales included 98.2% EA, 99.6% CA, 3.0% VME, and 0.2% ME. Evaluable EA was 80.6% but due to only 67 evaluable results. These findings support VITEK 2 MEV as an accurate automated system for MEV susceptibility testing of Enterobacterales and P. aeruginosa and could be an alternate solution to the manual labor intensive reference BMD method.

scholarly journals Performance of Fully Automated Antimicrobial Disk Diffusion Susceptibility Testing Using Copan WASP Colibri coupled to Radian in-Line Carousel and Expert System

2021 ◽  
Author(s):  
Abdessalam Cherkaoui ◽  
Gesuele Renzi ◽  
Nicolas Vuilleumier ◽  
Jacques Schrenzel
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Expert System ◽  
Enterococcus Faecalis ◽  
Susceptibility Testing ◽  
Disk Diffusion ◽  
Clinical Samples ◽  
Routine Laboratory ◽  
Coagulase Negative Staphylococci ◽  
Better Than

The purpose of the present study was to assess the agreement at the categorical level between the VITEK ® 2 system and the Colibri TM coupled to the Radian TM under real routine laboratory conditions. The 675 non-duplicate clinical strains included in this study (249 Enterobacterales-isolates, 198 Pseudomonas aeruginosa , 107 Staphylococcus aureus , 78 coagulase-negative staphylococci, 38 Enterococcus faecalis and 5 Enterococcus faecium ) were isolated from non-consecutive clinical samples referred to our laboratory between June and November 2020. In addition, 43 carbapenemase-producing Enterobacterales (CPE) formerly identified and stored in our laboratory were added to the panel, for a total of 718 strains. The overall categorical agreements between the two compared methods were 99.3% (4350/4380; 95% CI 99% - 99.5%); 98.6% (2147/2178; 95% CI 98.0% - 99.0%); 99.4% (1839/1850; 95% CI 98.9% - 99.7%); and 99.4% (342/344; 95% CI 97.9% - 99.8%) for Enterobacterales, P. aeruginosa , Staphylococcus spp. and Enterococcus spp. respectively. The most important cause of the very major errors encountered on the VITEK ® 2 for P. aeruginosa (62%, 13/21) was related to the presence of heteroresistant populations. Among the 43 CPE included in this study, one OXA-48-like, and one OXA-181-like were missed by the VITEK ® 2, even by rigorously applying the CPE screening cut-offs defined by EUCAST. The Colibri TM coupled to the Radian TM provide a fully automated solution for antimicrobial disk diffusion susceptibility testing with an accuracy that is equal to or better than that of the VITEK ® 2 system.

ATIVIDADE ANTIMICROBIANA IN VITRO DO EXTRATO AQUOSO, HIDROALCOÓLICO E ALCOÓLICO DE FOLHAS DE ESPÉCIES DA FAMÍLIA LAMIACEAE

2018 ◽  
Vol 4 ◽  
Author(s):  
Karlynne Freire Mendonça ◽  
José Klauber Roger Carneiro ◽  
Maria Auxiliadora Silva Oliveira
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Streptococcus Pyogenes ◽  
Mentha Arvensis ◽  
Ocimum Gratissimum ◽  
Mueller Hinton ◽  
Plectranthus Amboinicus

Objetivos: avaliar a atividade antimicrobiana em extrato aquoso, hidroalcoólico e alcoólico das folhas de espécies da família Lamiaceae frente a bactérias de interesse. Método: Foram escolhidas quatro espécies: Ocimum gratissimum, Plectranthus amboinicus, Mentha arvensis e Plectranthus barbatus. A partir das folhas foram confeccionados os extratos aquoso, hidroalcoólico e alcoólico nas concentrações 100mg/mL, 50mg/mL e 25mg/mL. Foram selecionadas as bactérias Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus aureus e Pseudomonas aeruginosa para os ensaios de antibiose em Ágar Mueller-Hinton. Resultados: P. barbatus, em seu extrato hidroalcoólico mostrou ativo nas três concentrações para bactéria S. aureus, e ainda foi ativo para P. aeruginosa, demonstrando no extrato alcoólico atividade frente as bactérias. Para M. arvensis e P. amboinicus, seus extratos hidroalcoólico e alcoólico apresentaram atividade para S. aureus. Conclusão: Sugere-se que as espécies em questão apresentem boa atividade antimicrobiana, sendo necessária a realização de mais estudos para melhor entender esse mecanismo.

Avaliação da atividade antimicrobiana do MTA e cimento portland em cepas de enterococcus faecalis e pseudomonnas aeruginosa

2012 ◽  
Author(s):  
Stéphanie Yone Antonio Abinader da Silva
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis

A determinação do perfil antimicrobiano de materiais endodônticos é de grande importância para determinação dos procedi-mentos odontológicos. O objetivo do presente trabalho foi avaliar o perfil antimicrobiano dos cimentos endodônticos MTA, que vem constituindo-se um excelente selador e preenchedor especialmente nas perfurações radiculares e de furca e que até então possuíam um prognóstico bastante desfavorável, e o Cimento Portland, que vem sendo utilizado com eficácia no tratamento da polpa dentária, frente a cepas de enterococcus faecalis e pseudomonas aeruginosa, bactérias comumente resistentes aos anti-microbianos atuais e encontradas na maioria dos casos de lesões endodônticas refratárias. No teste antimicrobiano, os cimentos foram diluídos em água destilada e aplicados em discos de papel de filtro estéreis que foram colocados em placas de Petri ino-culadas com as bactérias. O teste estatístico utilizado (ANOVA) permitiu inferir que houve uma diferença significante (p<0,05) entre os controles positivos e os cimentos avaliados. Com base nos resultados obtidos, pode se concluir que nenhum dos cimentos testados, MTA ou Portland, apresentam atividade antimicrobiana sobre as cepas testadas exigindo, portanto um protocolo de desinfecção prévio ao se utilizar esses materiais na prática odontológica.palavras-chave: MTA; cimento Portland; atividade antimicrobiana; enterococcus faecalis; pseudomonas aeruginosas; endo-dontia; microbiologia.

scholarly journals Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yong He ◽  
Hang Zhao ◽  
Yuanwen Liu ◽  
He Zhou
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Susceptibility ◽  
Magnetic Particles ◽  
Susceptibility Testing ◽  
Fluorescein Isothiocyanate ◽  
High Specificity ◽  
Antimicrobial Susceptibility Testing ◽  
Tail Fiber ◽  
Isolation And Identification ◽  
Magainin Ii

AbstractThe worldwide emergence and spread of antimicrobial resistance is accelerated by irrational administration and use of empiric antibiotics. A key point to the crisis is a lack of rapid diagnostic protocols for antimicrobial susceptibility testing (AST), which is crucial for a timely and rational antibiotic prescription. Here, a recombinant bacteriophage tail fiber protein (TFP) was functionalized on magnetic particles to specifically capture Pseudomonas aeruginosa, while fluorescein isothiocyanate-labeled-magainin II was utilized as the indicator. For solving the magnetic particles’ blocking effects, a reverse assaying protocol based on TFP recognition was developed to investigate the feasibility of detection and AST of P. aeruginosa. P. aeruginosa can be rapidly, sensitively and specifically detected within 1.5 h with a linear range of 1.0 × 102 to 1.0 × 106 colony forming units (CFU)⋅mL−1 and a detection limit of 3.3 × 10 CFU⋅mL−1. Subsequently, AST results, which were consistent with broth dilution results, can be obtained within 3.5 h. Due to the high specificity of the TFP, AST can actually be conducted without the need for bacterial isolation and identification. Based on the proof-of-principle work, the detection and AST of other pathogens can be extended by expressing the TFPs of their bacteriophages.

Sign in / Sign up

Export Citation Format

Share Document