Histochemical procedures for the simultaneous visualization of neutral sugars and either sialic acid and its side chainO-acyl variants orO-sulphate ester. I. Methods based upon the selective periodate oxidation of sialic acids

1987 ◽  
Vol 19 (5) ◽  
pp. 249-256 ◽  
Author(s):  
D. Volz ◽  
P. E. Reid ◽  
C. M. Park ◽  
D. A. Owen ◽  
W. L. Dunn
Keyword(s):  
Sialic Acid ◽  
Periodate Oxidation ◽  
Sialic Acids ◽  
Neutral Sugars ◽  
Selective Periodate Oxidation ◽  
Simultaneous Visualization

How selective are the ?selective periodate oxidation? methods used in sialic acid histochemistry?

1991 ◽  
Vol 23 (6) ◽  
pp. 290-292 ◽  
Author(s):  
J. Needham ◽  
P. E. Reid ◽  
D. A. Owen
Keyword(s):  
Sialic Acid ◽  
Periodate Oxidation ◽  
Selective Periodate Oxidation

scholarly journals A histochemical method for the identification of 9-O-acyl sialic acid. An investigation of bovine submaxillary gland and intestinal mucins.

1978 ◽  
Vol 26 (3) ◽  
pp. 187-192 ◽  
Author(s):  
P E Reid ◽  
C F Culling ◽  
W L Dunn
Keyword(s):  
Sialic Acid ◽  
Potassium Hydroxide ◽  
Periodic Acid ◽  
Periodate Oxidation ◽  
Submaxillary Gland ◽  
Sialic Acids ◽  
Histochemical Method ◽  
Periodic Acid Schiff ◽  
Oxidation Step ◽  
Intestinal Mucins

Prolongation of the initial periodate oxidation step of the periodate-borohydride technique/potassium hydroxide/periodic acid-Schiff and periodic acid-Thionin Schiff/potassium hydroxide/periodic acid-Schiff sequences produced little or no change in the diagnostic staining for the potassium hydroxide/periodic acid-Schiff effect, exhibited by the colonic epithelial mucins of man and rat and the Brunner's gland mucin of rabbits. In contrast, there was a gradual, but clear decrease in the intensity of such staining of bovine submaxillary gland mucins. It was concluded that, in the intestinal mucins studied the potassium hydroxide/periodic acid-Schiff effect was due to sialic acids bearing O-acyl substitutents at positions C7 and/or C8 whereas in bovine submaxillary gland mucin the potassium hydroxide/periodic acid-Schiff effect is probably due, at least in part, to the presence of 9-O-acyl sialic acids. This investigation has led to the development of a technique which can be used to identify 9-O-acyl sialic acids.

A new histochemical method for the selective periodate oxidation of total tissue sialic acids

1987 ◽  
Vol 19 (6-7) ◽  
pp. 311-318 ◽  
Author(s):  
D. Volz ◽  
P. E. Reid ◽  
C. M. Park ◽  
D. A. Owen ◽  
W. L. Dunn
Keyword(s):  
Periodate Oxidation ◽  
Sialic Acids ◽  
Histochemical Method ◽  
Total Tissue ◽  
Selective Periodate Oxidation

scholarly journals A new histochemical method for detection of sialic acids using a physical development procedure.

1995 ◽  
Vol 43 (10) ◽  
pp. 1045-1051 ◽  
Author(s):  
T Ueda ◽  
O Fujimori ◽  
K Yamada
Keyword(s):  
Periodic Acid ◽  
Periodate Oxidation ◽  
Physical Development ◽  
Sialic Acids ◽  
New Method ◽  
Histochemical Method ◽  
Reaction Products ◽  
Specificity And Sensitivity ◽  
Development Procedure ◽  
Selective Periodate Oxidation

We have established an efficient histochemical method for demonstration of sialic acids in light microscopy. The method consists of a selective periodate oxidation-phenylhydrazine blockade and a thiocarbohydrazide-silver protein sequence followed by a physical development procedure. From the results obtained by the present experimental and control studies in tissue sections from a series of rat and mouse organs, such as stomach, duodenum, colon, liver, sublingual gland, lung, and kidney, the specificity and sensitivity of the method were sufficient. In the tissues tested, sialic acids were visualized as distinct brownish and blackish reaction products. Comparisons of the new method with the periodic acid-phenylhydrazine-Schiff (PA-P-S) or selective periodate oxidation-Schiff (PA*-S) method employed hitherto have confirmed that the new method reported here is higher in efficiency and visibility of reaction products than the latter two methods.

Methods for the identification of side chainO-acyl substituted sialic acids and for the simultaneous visualization of sialic acid, its side chainO-acyl variants andO-sulphate ester

1987 ◽  
Vol 19 (6-7) ◽  
pp. 396-398 ◽  
Author(s):  
P. E. Reid ◽  
D. Volz ◽  
C. M. Park ◽  
D. A. Owen ◽  
W. L. Dunn
Keyword(s):  
Sialic Acid ◽  
Sialic Acids ◽  
Simultaneous Visualization

COMPARATIVE ACTION OF VARIOUS OESTROGENIC COMPOUNDS ON MOUSE VAGINAL SIALIC ACIDS (II)

1969 ◽  
Vol 62 (4) ◽  
pp. 663-670 ◽  
Author(s):  
Lars Carlborg
Keyword(s):  
Sialic Acid ◽  
Response Curve ◽  
Sialic Acids ◽  
Clear Cut ◽  
Suitable Parameter ◽  
Sufficient Degree ◽  
Comparative Action ◽  
Relative Potencies ◽  
Good Agreement

ABSTRACT Oestrogens administered in lower doses than necessary to induce full cornification of the mouse vagina induce mucification. It was shown previously that the degree of mucification could be estimated by quantitative determination of sialic acids. A suitable parameter for oestrogen assay was the measurement of vaginal sialic acid concentration which exhibited a clear cut dose response curve. Eleven assays of various oestrogens were performed with this method. Their estimated relative potencies were in good agreement with other routine oestrogen assays. A statistically sufficient degree of precision was found. The sensitivity was of the same order, or slightly higher, than the Allen-Doisy test.

Interspecific variation of zona pellucida glycoconjugates in several species of marsupial

Reproduction ◽  
2000 ◽  
pp. 111-120 ◽  
Author(s):  
JA Chapman ◽  
OW Wiebkin ◽  
WG Breed
Keyword(s):  
Glycine Max ◽  
Sialic Acid ◽  
Zona Pellucida ◽  
Fluorescein Isothiocyanate ◽  
Interspecific Variation ◽  
Lectin Histochemistry ◽  
Sialic Acids ◽  
Arachis Hypogea ◽  
Marsupial Species ◽  
Similar Intensity

The zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry. Ovaries from fat-tailed dunnarts, a southern brown bandicoot, grey short-tailed opossums, brushtail possums, ringtail possums, koalas and eastern grey kangaroos were fixed, embedded in paraffin wax, sectioned and stained with ten fluorescein isothiocyanate-conjugated lectins. Sections were also incubated with either neuraminidase or saponified, respectively, before incubation with the lectins to identify saccharide residues masked by sialic acids or O-acetyl groups on sialic acids. The zonae pellucidae surrounding the oocytes of the marsupials demonstrated interspecific variation in glycoconjugate content, with mannose-containing glycoconjugates exhibiting the greatest variation. Some of the zona pellucida glycoconjugates of all species, except those of the opossums, were masked by sialic acid with an increase in fluorescence with lectins from Arachis hypogea (PNA), and Glycine max (SBA), after desialylation. The disaccharide beta-galactose(1-4)N-acetyl-D-glucosamine appeared to be conformationally masked by O-acetyl groups of sialic acids in the zonae pellucidae of all species, with an increase in fluorescence with the lectin from Erythrina cristagalli (ECA), after saponification. Similar intensity and localization of beta-(1-4)-N-acetyl-D-glucosamine, as shown by staining of the lectin from Triticum vulgaris (WGA), to the inner and outer regions of the zona pellucida, were found to those reported in eutherian species. WGA fluorescence became uniform throughout the zonae pellucidae after saponification, indicating differential O-acetylation of sialic acids on the internal compartment of the zonae pellucidae.

scholarly journals Glycan-protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces

2021 ◽  
Author(s):  
Yixuan Xie ◽  
Siyu Chen ◽  
Qiongyu Li ◽  
Ying Sheng ◽  
Michael R Alvarez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sialic Acid ◽  
Membrane Proteins ◽  
Cell Membranes ◽  
Sialic Acids ◽  
Cross Linking ◽  
Protein Networks ◽  
Cell Surfaces ◽  
Protein Cross Linking

A cross-linking method is developed to elucidate the glycan-mediated interactions between membrane proteins through sialic acids. The method provides previously unknown extensive glycomic interactions on cell membranes. The vast majority...

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