Applications of the selective periodate oxidation of sialic acids III. Identification of neuraminidase-sensitive and neuraminidase-resistant sialic acids and their side chain O-acyl variants

1988 ◽  
Vol 20 (11) ◽  
pp. 645-650 ◽  
Author(s):  
P. E. Reid ◽  
C. Arratoon ◽  
D. A. Owen
Keyword(s):  
Periodate Oxidation ◽  
Sialic Acids ◽  
Side Chain ◽  
Selective Periodate Oxidation

A new histochemical method for the selective periodate oxidation of total tissue sialic acids

1987 ◽  
Vol 19 (6-7) ◽  
pp. 311-318 ◽  
Author(s):  
D. Volz ◽  
P. E. Reid ◽  
C. M. Park ◽  
D. A. Owen ◽  
W. L. Dunn
Keyword(s):  
Periodate Oxidation ◽  
Sialic Acids ◽  
Histochemical Method ◽  
Total Tissue ◽  
Selective Periodate Oxidation

scholarly journals A new histochemical method for detection of sialic acids using a physical development procedure.

1995 ◽  
Vol 43 (10) ◽  
pp. 1045-1051 ◽  
Author(s):  
T Ueda ◽  
O Fujimori ◽  
K Yamada
Keyword(s):  
Periodic Acid ◽  
Periodate Oxidation ◽  
Physical Development ◽  
Sialic Acids ◽  
New Method ◽  
Histochemical Method ◽  
Reaction Products ◽  
Specificity And Sensitivity ◽  
Development Procedure ◽  
Selective Periodate Oxidation

We have established an efficient histochemical method for demonstration of sialic acids in light microscopy. The method consists of a selective periodate oxidation-phenylhydrazine blockade and a thiocarbohydrazide-silver protein sequence followed by a physical development procedure. From the results obtained by the present experimental and control studies in tissue sections from a series of rat and mouse organs, such as stomach, duodenum, colon, liver, sublingual gland, lung, and kidney, the specificity and sensitivity of the method were sufficient. In the tissues tested, sialic acids were visualized as distinct brownish and blackish reaction products. Comparisons of the new method with the periodic acid-phenylhydrazine-Schiff (PA-P-S) or selective periodate oxidation-Schiff (PA*-S) method employed hitherto have confirmed that the new method reported here is higher in efficiency and visibility of reaction products than the latter two methods.

How selective are the ?selective periodate oxidation? methods used in sialic acid histochemistry?

1991 ◽  
Vol 23 (6) ◽  
pp. 290-292 ◽  
Author(s):  
J. Needham ◽  
P. E. Reid ◽  
D. A. Owen
Keyword(s):  
Sialic Acid ◽  
Periodate Oxidation ◽  
Selective Periodate Oxidation

scholarly journals Studies of the polysaccharide fraction from the lipopolysaccharide of Pseudomonas alcaligenes

1974 ◽  
Vol 139 (3) ◽  
pp. 633-643 ◽  
Author(s):  
James A. Lomax ◽  
George W. Gray ◽  
Stephen G. Wilkinson
Keyword(s):  
Gel Filtration ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Methylation Analysis ◽  
Mild Acid Hydrolysis ◽  
Pseudomonas Alcaligenes ◽  
Crude Polysaccharide ◽  
Hydrolysis Of ◽  
Mild Acid

Studies of the lipopolysaccharide of Pseudomonas alcaligenes strain BR 1/2 were extended to the polysaccharide moiety. The crude polysaccharide, obtained by mild acid hydrolysis of the lipopolysaccharide, was fractionated by gel filtration. The major fraction was the phosphorylated polysaccharide, for which the approximate proportions of residues were; glucose (2), rhamnose (0.7), heptose (2–3), galactosamine (1), alanine (1), 3-deoxy-2-octulonic acid (1), phosphorus (5–6). The heptose was l-glycero-d-manno-heptose. The minor fractions from gel filtration contained free 3-deoxy-2-octulonic acid, Pi and PPi. The purified polysaccharide was studied by periodate oxidation, methylation analysis, partial hydrolysis, and dephosphorylation. All the rhamnose and part of the glucose and heptose occur as non-reducing terminal residues. Other glucose residues are 3-substituted, and most heptose residues are esterified with condensed phosphate residues, possibly in the C-4 position. Free heptose and a heptosylglucose were isolated from a partial hydrolysate of the polysaccharide. The location of galactosamine in the polysaccharide was not established, but either the C-3 or C-4 position appears to be substituted and a linkage to alanine was indicated. In its composition, the polysaccharide from Ps. alcaligenes resembles core polysaccharides from other pseudomonads: no possible side-chain polysaccharide was detected.

4,6-O-(1-Carboxyethylidene)-D-mannose as a Structural Unit in Capsular Polysaccharide of Klebsiella K-type 5

1972 ◽  
Vol 50 (14) ◽  
pp. 2382-2384 ◽  
Author(s):  
G. G. S. Dutton ◽  
M. T. Yang
Keyword(s):  
Experimental Data ◽  
Structural Unit ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Acetyl Group

Methylation, periodate oxidation, and partial hydrolysis techniques have each been used to demonstrate the presence of 4,6-O-(1-carboxyethylidene)-D-mannopyranosyl units in the capsular polysaccharide of Klebsiella K-type 5. The structure of this polysaccharide differs from those known for other Klebsiella capsules by the lack of any carbohydrate side chain. A repeating unit of[Formula: see text](plus one unassigned O-acetyl group) is in accord with the experimental data.

scholarly journals A correlative chemical and histochemical study of the O-acetylated sialic acids of human colonic epithelial glycoproteins in formalin fixed paraffin embedded tissues.

1978 ◽  
Vol 26 (12) ◽  
pp. 1033-1041 ◽  
Author(s):  
P E Reid ◽  
C F Culling ◽  
W L Dunn ◽  
M G Clay ◽  
C W Ramey
Keyword(s):  
Epithelial Cells ◽  
Histochemical Study ◽  
Sialic Acids ◽  
Side Chain ◽  
Isolation And Identification ◽  
New Information ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Human Large Intestine ◽  
Formalin Fixed

Procedures are described for the isolation and identification of epithelial glycoproteins from formalin fixed, paraffin embedded specimens of human large intestine. The side chain O-acetylation patterns of the sialic acids of these glycoproteins were surprisingly similar to those of purified glycoproteins prepared from epithelial cells obtained from the same tissue before fixation. These results were consistent with those obtained by histochemical procedures performed on representative sections taken from the same tissue blocks. The methodology described permits a direct correlation of chemical and histochemical results obtained from the study of colonic epithelial glycoproteins of both normal and diseased tissues. It eliminates some of the difficulties associated with interpretation of the results by either discipline and may provide new information which would be unavailable by either chemistry or histochemistry alone.

EXOCELLULAR BACTERIAL POLYSACCHARIDE FROM XANTHOMONAS CAMPESTRIS NRRL B-1459: PART III. STRUCTURE

1964 ◽  
Vol 42 (6) ◽  
pp. 1261-1269 ◽  
Author(s):  
J. H. Sloneker ◽  
Danute G. Orentas ◽  
Allene Jeanes
Keyword(s):  
Sodium Borohydride ◽  
Xanthomonas Campestris ◽  
Glucuronic Acid ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Bacterial Polysaccharide ◽  
Salt Solutions ◽  
Anomalous Viscosity ◽  
Viscosity Behavior ◽  
Mild Acid

Periodate oxidation showed that the O-acetyl groups in the polysaccharide sterically affected the rate but not the extent of oxidation of the D-mannose residues, two-thirds of which were glycosidically substituted at C2 by a D-glucuronic acid residue and one-third of which was linked as a terminal side-chain residue. The D-glucose and D-glucuronic acid residues oxidized by periodate were substituted at C4, but both were more resistant to oxidation than were the D-mannose residues. One-third of the D-glucose residues and a significant quantity of the D-glucuronic acid residues were inert to vigorous periodate oxidation and may bear side-chain residues. Quantitative recovery of the periodate-stable D-glucose residues as 2-O-β-D-glucopyranosyl-D-erythritol, after the oxidized polysaccharide was reduced with sodium borohydride and hydrolyzed with mild acid, revealed that two-thirds of the D-glucose residues were in pairs linked (β, 1 → 4). The pyruvic acid linkage in the polysaccharide was established as a 4,6-O-1-carboxyethylidene ketal attached to a terminal D-glucose side-chain residue. The structure of the polysaccharide is discussed in relation to its anomalous viscosity behavior in salt solutions.

Biochemical engineering of the acyl side chain of sialic acids stimulates integrin-dependent adhesion of HL60 cells to fibronectin

2002 ◽  
Vol 80 (10) ◽  
pp. 671-677 ◽  
Author(s):  
Pablo Villavicencio-Lorini ◽  
Stephan Laabs ◽  
Kerstin Danker ◽  
Werner Reutter ◽  
Rüdiger Horstkorte
Keyword(s):  
Sialic Acids ◽  
Side Chain ◽  
Biochemical Engineering ◽  
Hl60 Cells ◽  
Acyl Side Chain

Structure of the polysaccharide chain of Pasteurella haemolytica (serotype 4) lipopolysaccharide

1984 ◽  
Vol 62 (2-3) ◽  
pp. 108-114 ◽  
Author(s):  
Malcolm B. Perry ◽  
Lorne A. Babiuk
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Cell Wall ◽  
Magnetic Resonance ◽  
Periodate Oxidation ◽  
Pasteurella Haemolytica ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Nuclear Magnetic Resonance Analysis ◽  
Resonance Analysis ◽  
Antigenic Polysaccharide

The antigenic polysaccharide side chain of the cell wall lipopolysaccharide of Pasteurella haemolytica (serotype 4) was investigated by methylation, periodate oxidation, partial hydrolysis, and 13C and 1H nuclear magnetic resonance analysis methods and was found to be a simple unbranched linear polymer composed of a disaccharide repeating unit having the structure —3)-α-D-Galp-(1—3)-β-D-Galf-(1—.

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