A comparison of the rate equations, kinetic parameters, and activation energies for the initial uptake ofl-lysine,l-valine, γ-aminobutyric acid, and α-aminoisobutyric acid by mouse brain slices

1975 ◽  
Vol 22 (1) ◽  
pp. 53-72 ◽  
Author(s):  
Stephen R. Cohen
Keyword(s):  
Kinetic Parameters ◽  
Mouse Brain ◽  
Brain Slices ◽  
Activation Energies ◽  
Aminobutyric Acid ◽  
Rate Equations ◽  
Aminoisobutyric Acid ◽  
Initial Uptake

scholarly journals Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices

2021 ◽  
Vol 2 (1) ◽  
pp. 100332
Author(s):  
Chirag Vasavda ◽  
Solomon H. Snyder ◽  
Bindu D. Paul
Keyword(s):  
Reactive Oxygen Species ◽  
Mouse Brain ◽  
Quantitative Measurement ◽  
Ex Vivo ◽  
Brain Slices ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices

2021 ◽  
Vol 2 (2) ◽  
pp. 100442
Author(s):  
Kevin M. Manz ◽  
Justin K. Siemann ◽  
Douglas G. McMahon ◽  
Brad A. Grueter
Keyword(s):  
Patch Clamp ◽  
Mouse Brain ◽  
Brain Slices ◽  
Electrode Array ◽  
Electrophysiological Analysis ◽  
Multi Electrode Array

Comparison of changes evoked by GABA (γ-aminobutyric acid) and anoxia in [K+]o, [Cl-]o, and [Na+]o in stratum pyramidale and stratum radiatum of the guinea pig hippocampus

2000 ◽  
Vol 78 (5) ◽  
pp. 378-391 ◽  
Author(s):  
G V Obrocea ◽  
M E Morris
Keyword(s):  
Guinea Pig ◽  
Extracellular Space ◽  
Hippocampal Slices ◽  
Brain Slices ◽  
Receptor Activation ◽  
Ion Concentration ◽  
Aminobutyric Acid ◽  
Stratum Radiatum ◽  
Bicuculline Methiodide ◽  
Stratum Pyramidale

Ion-selective microelectrode recordings were made to assess a possible contribution of extracellular γ-aminobutyric acid (GABA) accumulation to early responses evoked in the brain by anoxia and ischemia. Changes evoked by GABA or N2 in [K+]o, [Cl-]o, [Na+]o, and [TMA+]o were recorded in the cell body and dendritic regions of the stratum pyramidale (SP) and stratum radiatum (SR), respectively, of pyramidal neurons in CA1 of guinea pig hippocampal slices. Bath application of GABA (1-10 mM) for approximately 5 min evoked changes in [K+]o and [Cl-]o with respective EC50 levels of 3.8 and 4.1 mM in SP, and 4.7 and 5.6 mM in SR. In SP 5 mM GABA reversibly increased [K+]o and [Cl-]o and decreased [Na+]o; replacement of 95% O2 -5% CO2 by 95% N2 -5% CO2 for a similar period of time evoked changes which were for each ion in the same direction as those with GABA. In SR both GABA and N2 caused increases in [K+]o and decreases in [Cl-]o and [Na+]o. The reduction of extracellular space, estimated from levels of [TMA+]o during exposures to GABA and N2, was 5-6% and insufficient to cause the observed changes in ion concentration. Ion changes induced by GABA and N2 were reversibly attenuated by the GABAA receptor antagonist bicuculline methiodide (BMI, 100 µM). GABA-evoked changes in [K+]o in SP and SR and [Cl-]o in SP were depressed by >=90%, and of [Cl-]o in SR by 50%; N2-evoked changes in [K+]o in SP and SR were decreased by 70% and those of [Cl-]o by 50%. BMI blocked Δ [Na+]o with both GABA and N2 by 20-30%. It is concluded that during early anoxia: (i) accumulation of GABA and activation of GABAA receptors may contribute to the ion changes and play a significant role, and (ii) responses in the dendritic (SR) regions are greater than and (or) differ from those in the somal (SP) layers. A large component of the [K+]o increase may involve a GABA-evoked Ca2+-activated gk, secondary to [Ca2+]i increase. A major part of [Cl-]o changes may arise from GABA-induced gCl and glial efflux, with strong stimulation of active outward transport and anion exchange at SP, and inward Na+/K+/2Cl- co-transport at SR. Na+ influx is attributable mainly to Na+-dependent transmitter uptake, with only a small amount related to GABAA receptor activation. Although the release and (or) accumulation of GABA during anoxia might be viewed as potentially protectant, the ultimate role may more likely be an important contribution to toxicity and delayed neuronal death. Key words: brain slices, ion-selective microelectrodes, stratum pyramidale, stratum radiatum, bicuculline methiodide, extracellular space shrinkage.

Dopamine autoreceptor regulation of release and uptake in mouse brain slices in the absence of D3 receptors

Neuroscience ◽  
2002 ◽  
Vol 112 (1) ◽  
pp. 39-49 ◽  
Author(s):  
J.D Joseph ◽  
Y.-M Wang ◽  
P.R Miles ◽  
E.A Budygin ◽  
R Picetti ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Brain Slices ◽  
D3 Receptors ◽  
Dopamine Autoreceptor

Very rapid assay of gamma-aminobutyric acid in mouse brain regions within 3 minutes by high-performance liquid chromatography with electrochemical detection

1989 ◽  
Vol 21 (2) ◽  
pp. 115-121 ◽  
Author(s):  
Shigeo Murai ◽  
Hiromichi Nagahama ◽  
Hiroko Saito ◽  
Hiroki Miyate ◽  
Yoshikatsu Masuda ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
Mouse Brain ◽  
High Performance ◽  
Brain Regions ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Rapid Assay

Some characteristics of binding of γ-aminobutyric acid and acetylcholine to a synaptic vesicle fraction from mouse brain

1968 ◽  
Vol 9 (2) ◽  
pp. 231-252 ◽  
Author(s):  
Kinya Kuriyama ◽  
Eugene Roberts ◽  
Johan Vos
Keyword(s):  
Synaptic Vesicle ◽  
Mouse Brain ◽  
Aminobutyric Acid
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