A new method for the reconstitution of the anion transport system of the human erythrocyte membrane

1986 ◽  
Vol 90 (2) ◽  
pp. 123-135 ◽  
Author(s):  
Uwe Scheuring ◽  
Klaus Kollewe ◽  
Winfried Haase ◽  
Dieter Schubert
Keyword(s):  
Erythrocyte Membrane ◽  
Transport System ◽  
Human Erythrocyte ◽  
Anion Transport ◽  
New Method ◽  
Human Erythrocyte Membrane ◽  
Anion Transport System

scholarly journals The interaction of 3-deoxy-3-fluoro-d-glucose with the hexose-transport system of the human erythrocyte

1973 ◽  
Vol 135 (4) ◽  
pp. 773-777 ◽  
Author(s):  
G. J. Riley ◽  
N. F. Taylor
Keyword(s):  
Kinetic Parameters ◽  
Erythrocyte Membrane ◽  
Transport System ◽  
Dissociation Energy ◽  
Human Erythrocyte ◽  
Optical Method ◽  
Hexose Transport ◽  
Human Erythrocyte Membrane ◽  
Significant Release ◽  
Glucose Carrier

1. By using an optical method the kinetic parameters of hexose transport across the human erythrocyte membrane were determined for several sugars. The series of half-saturated constants is as follows: 3-deoxy-3-fluoro-d-glucose = 3-O-methyl-d-glucose <d-glucose<d-mannose <3-deoxy-d-glucose<d-galactose<l-arabinose. 2. Estimations of the dissociation energy of the 3-deoxy-3-fluoro-d-glucose–carrier and d-glucose–carrier complexes suggest that the binding of the two sugars to the transport system is equivalent. 3. Incubation of the erythrocytes with 3-deoxy-3-fluoro-d-glucose results in a small but significant release of F−anion. Cells treated in this way lose their ability to transport glucose.

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