Seed transmission ofPyrenophora tritici-repentis, causal fungus of tan spot of wheat

1995 ◽  
Vol 101 (1) ◽  
pp. 81-91 ◽  
Author(s):  
A. M. C. Schilder ◽  
G. C. Bergstrom
1993 ◽  
Vol 59 (2) ◽  
pp. 175-179
Author(s):  
Wataru HASAMA ◽  
Tokuhiro KATO ◽  
Suzumi MORITA

2015 ◽  
Vol 68 ◽  
pp. 449-449
Author(s):  
S.K. Weith ◽  
H.J. Ridgway ◽  
I.C. Harvey ◽  
R.A. Craigie ◽  
E.E. Jones

Tan spot of wheat is caused by the fungus Pyrenophora triticirepentis (Ptr) with reported incidence in New Zealand increasing in recent years Lesions characteristic of Ptr infection being oval tan spots surrounded by a chlorotic halo were observed on wheat leaves in 15 different wheat paddocks throughout the South Island in the 201314 season Fungal isolates recovered from these lesions on potato dextrose agar produced greygreen fluffy mycelium characteristic of Ptr Speciesspecific PCR using published primers (PtrUniqueF2/ PtrUniqueR2) generated a PCR product of 490 bp diagnostic of Ptr Sequencing of the rDNA and 946;tubulin gene regions confirmed the identification Ptr was found to be widely distributed throughout the wheat growing areas in the South Island from Southland to North Canterbury A postharvest farmer questionnaire was also undertaken Questionnaire answers revealed that continuous sowing of untreated wheat seed conservative tillage and low rate applications of fungicides may have exacerbated the severity of tan spot outbreaks during the 201314 season Further work to identify susceptible wheat cultivars and sensitivity to fungicides is currently under way


Nucleus ◽  
2015 ◽  
Vol 12 (1) ◽  
Author(s):  
Gabrielle de Lima MASSON ◽  
Bruno Agostini COLMAN ◽  
Paulo Rogério Beltramin da FONSECA ◽  
Alexandre Dinnys ROESE

2019 ◽  
Vol 7 (6) ◽  
pp. 809-818 ◽  
Author(s):  
Wenjing Hu ◽  
Xinyao He ◽  
Susanne Dreisigacker ◽  
Carolina P. Sansaloni ◽  
Philomin Juliana ◽  
...  

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 139
Author(s):  
Sharon A. Andreason ◽  
Omotola G. Olaniyi ◽  
Andrea C. Gilliard ◽  
Phillip A. Wadl ◽  
Livy H. Williams ◽  
...  

Sweet potato leaf curl virus (SPLCV) threatens global sweet potato production. SPLCV is transmitted by Bemisia tabaci or via infected vegetative planting materials; however, SPLCV was suggested to be seed transmissible, which is a characteristic that is disputed for geminiviruses. The objective of this study was to revisit the validity of seed transmission of SPLCV in sweet potato. Using large-scale grow-out of sweet potato seedlings from SPLCV-contaminated seeds over 4 consecutive years, approximately 23,034 sweet potato seedlings of 118 genotype entries were evaluated. All seedlings germinating in a greenhouse under insect-proof conditions or in a growth chamber were free of SPLCV; however, a few seedlings grown in an open bench greenhouse lacking insect exclusion tested positive for SPLCV. Inspection of these seedlings revealed that B. tabaci had infiltrated the greenhouse. Therefore, transmission experiments were conducted using B. tabaci MEAM1, demonstrating successful vector transmission of SPLCV to sweet potato. Additionally, tests on contaminated seed coats and germinating cotyledons demonstrated that SPLCV contaminated a high percentage of seed coats collected from infected maternal plants, but SPLCV was never detected in emerging cotyledons. Based on the results of grow-out experiments, seed coat and cotyledon tests, and vector transmission experiments, we conclude that SPLCV is not seed transmitted in sweet potato.


Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 513
Author(s):  
Pao Theen See ◽  
Caroline S. Moffat

After nearly 40 years of DNA molecular marker development in plant breeding, the wheat research community has amassed an extensive collection of molecular markers which have been widely and successfully used for selection of agronomic, physiological and disease resistance traits in wheat breeding programs. Tan spot is a major fungal disease of wheat and a significant global economic challenge and is caused by the necrotrophic fungal pathogen Pyrenophora tritici-repentis (Ptr). Here, the potential for using a PCR-based marker (Ta1AS3422) present on the short arm of wheat chromosome 1A, was evaluated for effectiveness in distinguishing tan spot disease susceptibility. The marker was initially screened against 40 commercial Australian hexaploid wheat varieties, and those that amplified the marker had an overall lower disease score (2.8 ± 0.7 for seedlings and 2.4 ± 0.4 for plants at the tillering stage), compared to those lacking the marker which exhibited a higher disease score (3.6 ± 0.8 for both growth stages). The potential of Ta1AS3422 as a marker for the tan spot disease response was further assessed against a panel of 100 commercial Australian hexaploid wheat varieties. A significant association was observed between marker absence/presence and tan spot disease rating (Pearson’s chi-squared test, χ2 (6) = 20.53, p = 0.002), with absence of Ta1AS3422 associated with susceptibility. This simple and cost-effective PCR-based marker may be useful for varietal improvement against tan spot, although further work is required to validate its effectiveness.


Author(s):  
Oksana Yu. Kremneva ◽  
Nina V. Mironenko ◽  
Galina V. Volkova ◽  
Olga A. Baranova ◽  
Yuri S. Kim ◽  
...  

2019 ◽  
Vol 235 ◽  
pp. 27-37 ◽  
Author(s):  
Matías Schierenbeck ◽  
María Constanza Fleitas ◽  
Facundo Cortese ◽  
Silvina Inés Golik ◽  
María Rosa Simón

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