In vitro morphological characterization of the bursal reticuloepithelial (REp) cells of chicken

1990 ◽  
Vol 46 (10) ◽  
pp. 1060-1063 ◽  
Author(s):  
F. Giannessi ◽  
F. Bianchi ◽  
A. Dolfi ◽  
M. Lupetti ◽  
S. Michelucci
Keyword(s):  
Morphological Characterization

Density and morphological characterization of bacteria in integrated and exclusive production systems

2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino
Keyword(s):  
Bacterial Community ◽  
Rainy Season ◽  
Production Systems ◽  
Cropping System ◽  
Morphological Characterization ◽  
Native Forest ◽  
Exclusive Production ◽  
Colony Forming Units

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season

scholarly journals Fabrication and Characterization of Scaffolds of Poly(ε-caprolactone)/Biosilicate® Biocomposites Prepared by Generative Manufacturing Process

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Daniel Aparecido Lopes Vieira da Cunha ◽  
Paulo Inforçatti Neto ◽  
Kelli Cristina Micocci ◽  
Caroline Faria Bellani ◽  
Heloisa Sobreiro Selistre-de-Araujo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Manufacturing Process ◽  
Potential Application ◽  
Morphological Characterization ◽  
Mechanical Compression ◽  
Compression Tests ◽  
Extrusion Head ◽  
Fabrication And Characterization

Scaffolds of poly(ε-caprolactone) (PCL) and their biocomposites with 0, 1, 3, and 5 wt.% Biosilicate® were fabricated by the generative manufacturing process coupled with a vertical miniscrew extrusion head to application for restoration of bone tissue. Their morphological characterization indicated the designed 0°/90° architecture range of pore sizes and their interconnectivity is feasible for tissue engineering applications. Mechanical compression tests revealed an up to 57% increase in the stiffness of the scaffold structures with the addition of 1 to 5 wt.% Biosilicate® to the biocomposite. No toxicity was detected in the scaffolds tested by in vitro cell viability with MC3T3-E1 preosteoblast cell line. The results highlighted the potential application of scaffolds fabricated with poly(ε-caprolactone)/Biosilicate® to tissue engineering.

Morphological characterization of the adherence and invasion ofStreptococcus agalactiaeto the intestinal mucosa of tilapiaOreochromissp.: An in vitro model

2019 ◽  
Vol 42 (9) ◽  
pp. 1223-1231 ◽  
Author(s):  
Gersson Vásquez‐Machado ◽  
Paola Barato‐Gómez ◽  
Carlos Iregui‐Castro
Keyword(s):  
Intestinal Mucosa ◽  
In Vitro Model ◽  
Morphological Characterization ◽  
Vitro Model

scholarly journals Morphological characterization of pre- and peri-implantation in vitro cultured, somatic cell nuclear transfer and in vivo derived ovine embryos

Reproduction ◽  
2005 ◽  
Vol 130 (5) ◽  
pp. 681-694 ◽  
Author(s):  
P Tveden-Nyborg ◽  
T T Peura ◽  
K M Hartwich ◽  
S K Walker ◽  
P Maddox-Hyttel
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Morphological Characterization ◽  
Morphological Development ◽  
Embryonic Disc ◽  
Transmission Electron

The processes of cellular differentiation were studied in somatic cell nuclear transfer (SCNT), in vitro cultured (IVC) and in vivo developed (in vivo) ovine embryos on days 7, 9, 11, 13, 17 and 19. SCNT embryos were constructed from in vitro matured oocytes and granulosa cells, and IVC embryos were produced by in vitro culture of in vivo fertilized zygotes. Most SCNT and IVC embryos were transferred to recipients on day 6 while some remained in culture for day 7 processing. In vivo embryos were collected as zygotes, transferred to intermediate recipients and retransferred to final recipients on day 6. All embryos were processed for examination by light and transmission electron microscopy or immunohistochemical labelling for alpha-1-fetoprotein and vimentin. Overall, morphological development of in vivo embryos was superior to IVC and SCNT embryos. Day 7 and particularly day 9 IVC and SCNT embryos had impaired hypoblast development, some lacking identifiable inner cell masses. On day 11, only in vivo and IVC embryos had developed an embryonic disc, and gastrulation was evident in half of in vivo embryos and one IVC embryo. By day 13, all in vivo embryos had completed gastrulation whereas IVC and SCNT embryos remained retarded. On days 17 and 19, in vivo embryos had significantly more somites and a more developed allantois than IVC and SCNT embryos. We conclude that IVC and particularly SCNT procedures cause a retardation of embryo development and cell differentiation at days 7–19 of gestation.

scholarly journals IN VIVO AND IN VITRO STUDIES ON MORPHOLOGICAL CHARACTERIZATION OF SEPTORIA LYCOPERSICI CAUSING LEAF SPOT DISEASE IN TOMATO

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Aabid . ◽  
Sabiha Ashraf ◽  
Hilal A. Malik ◽  
Rakshanda Zargar ◽  
Shaheena A. Nagoo ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Morphological Characterization ◽  
Leaf Spot Disease ◽  
Potato Dextrose Agar Medium ◽  
Spot Disease ◽  
Spore Mass ◽  
Septoria Lycopersici

Septoria lycopersici responsible for Septoria leaf spot disease was observed on the leaves of tomato. Septoria lycopersici was isolated and completion of Koch’s postulates confirmed that the fungus was causal agent of the leaf spot disease. The fungus was cultured on potato dextrose agar medium. The fungus was very slow growing with 8-12 mm radial growth as recorded after 30 days of incubation. The fungus produced off white, irregular, hardened blackish mycelial growth oozing spore mass from pycnidia. Pycnidia were dark brown to black, globose to sub globose, ostiolated and thick walled. Pycnidiospores were filiform, straight with pointed to rounded ends.

scholarly journals Density and morphological characterization of bacteria in integrated and exclusive production systems

2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino
Keyword(s):  
Bacterial Community ◽  
Rainy Season ◽  
Production Systems ◽  
Cropping System ◽  
Morphological Characterization ◽  
Native Forest ◽  
Exclusive Production ◽  
Colony Forming Units

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season.

scholarly journals Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 359-369 ◽  
Author(s):  
Miljan Vasić ◽  
Ivana Vico ◽  
Wayne M. Jurick ◽  
Nataša Duduk
Keyword(s):  
Pathogen Detection ◽  
Management Strategies ◽  
Brown Rot ◽  
Morphological Characterization ◽  
Apple Fruit ◽  
Postharvest Disease ◽  
Pome Fruit

Brown rot, caused by Monilinia spp., is an economically important pre- and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre- and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.

scholarly journals Morphoagronomic characterization of Sorghum cultivar ‘CIAP 132R-05’ obtained by somatic embryogenesis in the conversion phase using different substrates

2019 ◽  
pp. 248
Author(s):  
Silvio de J. Martínez Medina ◽  
Raúl Barbón Rodríguez, Rafael Gómez-Kosky, Novisel Veitía Rodríguez ◽  
María Esther González Vega ◽  
Orlando Saucedo Castillo ◽  
Eduardo Fidel Héctor Ardisana ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Height ◽  
Somatic Embryos ◽  
Morphological Characters ◽  
Morphological Characterization ◽  
Stem Diameter ◽  
Limb Length ◽  
Regenerated Plants

Sorghum [Sorghum bicolor (L.) Moench] is an important crop after wheat, corn, rice and barley. The aim of this research was to study morphological characterization of the plant population of grain sorghum cultivar CIAP 132R-05 regenerated via somatic embryogenesis under semi controlled conditions in green house.  Plants regenerated from somatic embryos were compared to plants grown from botanical seeds. Both populations of plants were characterized morphologically, taking in account quantitative traits (plant height (cm), stem diameter, number of active leaves per plant, limb length and width, number of active roots per plant, length of the roots (cm), fresh weight (gMF), foliar area (dm2), number of shoots per plant) and qualitative traits (albino or variegated plants, color of the leaves). Quantitative characteristics of both populations corresponded with those listed in the National Register of Commercial Varieties of Cuba. However; the population of plants derived from somatic embryos showed significantly higher values ​​for plant height, stem diameter, limb length and width, which may be associated with the physiological rejuvenation produced by the effect of in vitro culture. This allowed to determine the phenotypic stability of the regenerated plants via somatic embryogenesis, by assessing morphological characters in field conditions. The results can be applied to the in vitro propagation of elite plants selected as a product of conventional breeding programs and obtained through the use of different biotechnological methods.

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