The effect of bovine serum albumin on the in vitro inhibition of chemotaxis by anti-inflammatory agents

1977 ◽  
Vol 7 (4) ◽  
pp. 465-468 ◽  
Author(s):  
Israel Rivkin
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
In Vitro Inhibition ◽  
Anti Inflammatory

scholarly journals Pharmacological and ADMET-based pharmacokinetic properties of Syzygium samarangense var. parviflorum leaf extract in in vitro, in vivo and in silico models

2020 ◽  
Vol 48 (3) ◽  
pp. 1155-1175
Author(s):  
Rahni HOSSAIN ◽  
Md A. RAHMAN ◽  
Md. K. J. RAFI ◽  
Tanvir A. SIDDIQUE ◽  
Abdullah A. NOMAN ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Computational Models ◽  
Clot Lysis ◽  
Pharmacological Properties ◽  
Reference Drug ◽  
Egg Albumin ◽  
Anti Inflammatory

This research investigated pharmacological properties mainly the anti-inflammatory, anthelmintic, thrombolytic and anxiolytic potential of methanol extract of Syzygium samarangense (MESS) var. parviflorum. Anti-inflammatory action by bovine serum albumin, egg albumin denaturation and membrane stabilization, anthelmintic by live parasites, thrombolytic by clot lysis and anxiolytic by elevated plus maze (EPM) and light and dark box (LDB) tests were measured. The four targeted pharmacological properties were further justified using the most prevalent compounds, isolated from this plant, to be undergone for their pharmacokinetic property’s analyses, sitemap analyses and lignad-receptor interactions by computational models through SwissADME and Schrödinger, 2018 softwares against PDB 6COX, 6D6T, 1JFF receptors. MESS was found to display statistically significant (P < 0.05) inhibition of Bovine Serum albumin and Egg albumin denaturation compared to reference drug diclofenac sodium. Remarkable vermicidal effect on the paralysis and death of anthelmintic parasites was observed at MESS concentration 200 mg/dL. A nondescript clot lysis of MESS compared to streptokinase was evident in in vitro thrombolytic assay. MESS increased the number of times the animal crossed from one compartment to the other and the time spent in the brightly-lit chamber of the LDB. Three-methylchalcone derivatives out of seven MESS compounds were undertaken, based on cut off value and sitemap prediction score, for further ligand-receptor binding efficiency. All these three compounds showed promising docking score, glide emodel and glide energy against PDB 6COX, 6D6T and 1DDJ, plasmin proteins demonstrating the prospects of MESS to be materialized for anti-inflammatory, anthelmintic, and thrombolytic therapeutics with further clarification.

scholarly journals Phytochemical Study of Aqueous Extract of Ochna schweinfurthiana F. Hoffm Powder Bark and Evaluation of Their Anti-Inflammatory, Cytotoxic, and Genotoxic Properties

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Steve V. Djova ◽  
Maximilienne A. Nyegue ◽  
Angelique N. Messi ◽  
Alian D. Afagnigni ◽  
François-X. Etoa
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Salmonella Typhimurium ◽  
Aqueous Extract ◽  
Bovine Serum ◽  
Moderate Activity ◽  
Traditional Use ◽  
Phytochemical Study ◽  
Anti Inflammatory

Ochna schweinfurthiana has been used in traditional medicine to treat pain, inflammation, and arthritis. It is a rich source of complex dimers of flavonoids with potential use as templates for the development of therapeutic drugs. Hence, the aim of this study was to study the phytochemical content and evaluate the in vitro cytotoxic, genotoxic, and anti-inflammatory activities of the aqueous extract of Ochna schweinfurthiana bark (OSE). Phytochemical study was carried out according to LC-MS procedures, while isolation was carried out using thin layer and column chromatographies. Cytotoxicity was investigated by the mitochondrial viability [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) method while genotoxicity potential of the extract was ascertained using the Salmonella typhimurium test strains TA98 and TA100. The anti-inflammatory effect of OSE was evaluated by the in vitro inhibition of 15-lipooxygenase enzyme and bovine serum albumin denaturation (BSA) assays. The investigation of compounds extracted from OSE led to the identification and isolation of six known compounds, namely, hemerocallone (9), 6,7-dimethoxy-3’-4’-dimethoxyisoflavone (10), lithospermoside (13), amentoflavone (14), agathisflavone (15), and β-D-fructofuranosyl-α-D-glucopyranoside (17). In the anti-inflammatory assay, aqueous extracts of the bark showed selective inhibition of 15-lipooxygenase with IC50 value of 32.2±0.36  μg/mL and the result of the bovine serum albumin denaturation assay with IC50 value of 130± 5.78 μg/mL showed moderate activity. The toxicity assay indicated that OSE are noncytotoxic on Vero cell line with LC50 value of 50 mg/mL and nongenotoxic toward Salmonella typhimurium tester strain TA98 and TA100. Result from this study supports the traditional use of the selected medicinal plants in Cameroon for the treatment of inflammatory conditions. Noncytotoxicity and nongenotoxicity of OSE suggest that this plant is safe for use.

scholarly journals Evaluation of in vitro anti-inflammatory activity of the spadix of Colocasia affinis

2019 ◽  
Vol 19 (2) ◽  
pp. 30-34
Author(s):  
Vanlalhruaii ◽  
C. Malsawmtluangi ◽  
H. Lalhlenmawia
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Methanolic Extract ◽  
Inflammatory Activity ◽  
Dependent Manner ◽  
Protease Inhibition ◽  
Egg Albumin ◽  
Anti Inflammatory

The methanolic extract of C. affinis was evaluated for its in-vitro anti-inflammatory activity by bovine serum albumin denaturation method, egg albumin denaturation method and protease inhibition method at different concentrations. Diclofenac sodium was used as the reference drug. The extract exhibits anti-inflammatory activity in a concentration-dependent manner. In bovine serum albumin denaturation method, the extract at concentrations of 100, 200, 400, 600, 800, 1000 µg/ml showed 25.49, 28.43, 31.37, 37.25, 41.17, 48.03% inhibition respectively. In egg albumin denaturation method, the concentrations of 50, 100, 200, 300, 400, 500 µg/ml showed 5.3, 9.89, 28.12, 32.8, 43.2, 52.8% inhibition respectively. In protease inhibition method the extract at concentration of 100, 200, 300, 400, 500 µg/ml showed 8.87, 19.32, 28.56, 43.96, 59.92% inhibition respectively. Therefore, from the results it can be concluded that the methanolic extract of C. affinis possesses anti-inflammatory activity.

A Precipitin-like Reaction of the Hemolymph of the Lobster Homarus americanus

1969 ◽  
Vol 26 (5) ◽  
pp. 1392-1397 ◽  
Author(s):  
James E. Stewart ◽  
Diane M. Foley
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Test Period ◽  
Fluorescent Material

The levels of fluorescent material in the hemolymph of lobsters injected with serum proteins from lobster hemolymph labelled with fluorescein remained relatively constant over a 6-day test period; the levels in lobsters injected with bovine serum albumin labelled with fluorescein declined rapidly. A precipitin-like reaction was observed when lobster hemolymph serum was titrated with bovine serum albumin in vitro.

Preparation and in vitro photodynamic activities of novel axially substituted silicon (IV) phthalocyanines and their bovine serum albumin conjugates

2006 ◽  
Vol 16 (9) ◽  
pp. 2450-2453 ◽  
Author(s):  
Xiong-Jie Jiang ◽  
Jian-Dong Huang ◽  
Yu-Jiao Zhu ◽  
Fen-Xiang Tang ◽  
Dennis K.P. Ng ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum

Amuchina Gel Xgerm hand rub in vitro virucidal activity against SARS-CoV-2

2021 ◽  
Author(s):  
Alessandra Capezzone de Joannon ◽  
Angela Testa ◽  
Natalie Falsetto ◽  
Michela Procaccini ◽  
Lorella Ragni
Keyword(s):  
Detection Limit ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Virucidal Activity ◽  
European Standard ◽  
Enveloped Viruses ◽  
Prevention Measure ◽  
Study Product

Aim: Ethanol is highly effective at inactivating enveloped viruses, including SARS-CoV-2. The aim of this study is to evaluate the virucidal activity of Amuchina Gel Xgerm (74% ethanol) against SARS-CoV-2, according to the European Standard EN14476:2013+A2:2019. Materials & methods: Virucidal activity of the study product was evaluated against SARS-CoV-2 strain USAWA1/2020 in suspension, in the presence of 0.3 g/l of bovine serum albumin. Results: The log10 reduction of SARS-CoV-2 in the presence of bovine serum albumin was ≥4.11 ± 0.12 after 30 s of exposure to the study product (80% dilution). Cytotoxicity was observed in the 100 dilution, affecting the detection limit by 1 log10. Conclusion: Virucidal activity against SARS-CoV-2 supports the effectiveness of this alcohol-based formulation as a prevention measure for COVID-19 illness.

scholarly journals In-vitro displacement interaction of atenolol and amlodipine on binding with bovine serum albumin when co-administered

2008 ◽  
Vol 2 (1) ◽  
Author(s):  
Md Ashraful Alam ◽  
Md Abdul Awal ◽  
Mahbub Mostofa ◽  
Md Kamrul Islam ◽  
Nusrat Subhan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum

Bovine serum albumin (BSA) can replace patient serum as a protein source in an in vitro fertilization (IVF) program

1989 ◽  
Vol 6 (3) ◽  
pp. 164-167 ◽  
Author(s):  
Claudio A. Benadiva ◽  
Barbara Kuczynski-Brown ◽  
Tobi G. maguire ◽  
Luigi Mastroianni ◽  
George L. Flickinger
Keyword(s):  
In Vitro Fertilization ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Protein Source ◽  
Vitro Fertilization

In Vitro Study of the Binding of Taxifolin to Bovine Serum Albumin and the Influence of Common Ions on the Binding

2010 ◽  
Vol 39 (4) ◽  
pp. 482-494 ◽  
Author(s):  
Xiaolei Shi ◽  
Xuwen Li ◽  
Yantao Sun ◽  
Wei Wei ◽  
Ruijie Yang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
In Vitro Study ◽  
Vitro Study
Sign in / Sign up

Export Citation Format

Share Document