Rapid identification ofClostridium difficile by determination of leucine arylamidase activity

1986 ◽  
Vol 5 (2) ◽  
pp. 160-161
Author(s):  
A. Kudhair ◽  
M. Delmée ◽  
G. Wauters
Keyword(s):  
Rapid Identification ◽  
Ofclostridium Difficile ◽  
Leucine Arylamidase

Micromethod for biochemical identification of coagulase-negative staphylococci

1978 ◽  
Vol 8 (5) ◽  
pp. 503-508
Author(s):  
Y Brun ◽  
J Fleurette ◽  
F Forey
Keyword(s):  
Excellent Agreement ◽  
Clinical Microbiology ◽  
Reference Method ◽  
Rapid Identification ◽  
Suitable Method ◽  
Coagulase Negative Staphylococci ◽  
Biochemical Identification ◽  
Type Strains

We have endeavored to elaborate a suitable method for easy and rapid identification in clinical microbiology laboratories of the different species of infection-inducing, coagulase-negative staphylococci. Ten type strains described by Kloos and Schleifer and 269 strains isolated from 95 patients were tested; the classical tests were used for determination of Staphylococcus species. Strains were identified by using the Kloos-Schleifer reference method and the micromethod simultaneously. After preliminary tests on 77 substrates, 19 were retained, 15 for determination of species and 4 to reveal biotypes. The substrates were placed in wells in a rigid strip of inert plastic. Inoculation of wells was carried out with rich microbial suspensions in a special medium; reading of substrate reactions was done after incubation for 48 h at 35 degree C. The intrasystem reproducibility was excellent, from 91 to 100% for the 19 substrates. It was in excellent agreement with the reference method, 100% for type strains and 97.9% for hospital-isolated strains. Because it is simple and easy to reproduce, the micromethod will be most useful in clinical and ecological microbiology laboratories.

Prior Determination of Flash Floods: Artificial Intelligence Based Predictive Analysis Using Modified Cuckoo Search

2020 ◽  
Vol 17 (2) ◽  
pp. 990-995
Author(s):  
Talha Ahmed Khan ◽  
Muhammad Alam ◽  
Kushsairy Kadir ◽  
Zeeshan Shahid ◽  
M. S. Mazliham
Keyword(s):  
Artificial Intelligence ◽  
Water Pressure ◽  
Cuckoo Search ◽  
Flash Floods ◽  
Rapid Identification ◽  
Predictive Analysis ◽  
Direct Observations ◽  
The Pacific ◽  
The Pacific Ocean

Flash floods are considered as the most intense hazard therefore rapid identification is needed. Tsunami also causes flash floods as it is commonly generated around the Pacific Ocean. Flash floods are also caused by the severely blocked streams in heavy rainfall. Floods have ended up so many lives more than the other natural hazards and also devastated precious belongings and infrastructures. Cattles have also been affected by the floods event. Floods devastate the construction and infrastructure like roads, bridges and buildings that comes in the vicinity of effected area by flood. Breakdown and overflow of dams may produce the deadly flash floods to the populated area and environs. Many strategies and methods have been followed to determine the flash floods on early basis so that evacuation announcements may be propagated. Numerous researches have been studied and carried out to accomplish this task. Development of dams and reservoirs have been given more significance. Artificial Intelligence based competent decision-making algorithms like Bayesian classifier, PSO, ANN, NNARX, SVM and GA have been applied to achieve more accuracy in predictive analysis. Direct observations from the sensors and data from the meteorological department have also been used for the predictive analysis of flash floods. Many yardstick parameters have been proposed in past researches to identify the flash floods vigorously like environmental CO2 levels, precipitation velocity, wind speed, upstream level, height of the water, pressure, temperature and cloud to ground flashes. In this research papers a novel Artificial Intelligence based approach Modified Cuckoo Search (MCS) has been adopted to forecast the flash floods more rapidly and accurately. Obtained results in the MATLAB have proved that Modified cuckoo search with the combination of Artificial Neural Network worked better than the recent available methods. Results have also been validated by comparing the MLP-PSO.

A synergic approach of X-ray powder diffraction and Raman spectroscopy for crystal structure determination of 2,3-thienoimide capped oligothiophenes

2018 ◽  
Vol 20 (5) ◽  
pp. 3630-3636 ◽  
Author(s):  
C. Cappuccino ◽  
P. P. Mazzeo ◽  
T. Salzillo ◽  
E. Venuti ◽  
A. Giunchi ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Raman Spectroscopy ◽  
Powder Diffraction ◽  
Structure Determination ◽  
Molecular Conformation ◽  
Rapid Identification ◽  
Crystal Structure Determination ◽  
X Ray

This work presents a Raman based approach for the rapid identification of the molecular conformation in a series of new 2,3-thienoimide capped quaterthiophenes.

scholarly journals Identification and Quantitation of Polymyxin B, Framycetin, and Dexamethasone in an Ointment by Using Thin-Layer Chromatography with Densitometry

2005 ◽  
Vol 88 (5) ◽  
pp. 1549-1554 ◽  
Author(s):  
Jan Krzek ◽  
Anna Maślanka ◽  
Pawel Lipner
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Polymyxin B ◽  
Rapid Identification ◽  
Mobile Phases ◽  
Relative Standard ◽  
Dexamethasone Acetate ◽  
Similar Accuracy ◽  
Layer Chromatography

Abstract A new thin-layer chromatographic-densitometric method has been developed for rapid identification and quantitative determination of polymyxin B, framycetin, and dexamethasone in a dental ointment. Silica gel 60 and F254 silica gel 60 plates were used for separating antibiotics and dexamethasone acetate, respectively. When determining framycetin and polymyxin B, chromatograms were developed by using 2 mobile phases, namely methanol and methanol–n-butanol–ammonia (25%)–chloroform (14 + 4 + 9 + 12, v/v/v/v/). The densitometric measurements were made at 550 nm after detection with 0.3% ninhydrin solution. Dexamethasone was determined by using the mobile phase cyclohexane–ethyl acetate (2 + 3, v/v) and ultraviolet densitometric recording at 245 nm. The results obtained for individual constituents with the chromatographic-densitometric method demonstrate similar accuracy, relative standard deviation values from 1.49 to 2.47%, and relative error values from 0.02 to 0.81% and are comparable to those obtained with the reference methods.

Rapid identification and determination of residues of chlorinated pesticides in crops by gas-liquid chromatography

The Analyst ◽  
1962 ◽  
Vol 87 (1032) ◽  
pp. 169 ◽  
Author(s):  
E. S. Goodwin ◽  
R. Goulden ◽  
J. G. Reynolds
Keyword(s):  
Liquid Chromatography ◽  
Rapid Identification ◽  
Gas Liquid Chromatography ◽  
Chlorinated Pesticides

scholarly journals Analysis of FlavoneC-Glycosides in the Leaves ofClinacanthus nutans(Burm. f.) Lindau by HPTLC and HPLC-UV/DAD

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
June Lee Chelyn ◽  
Maizatul Hasyima Omar ◽  
Nor Syaidatul Akmal Mohd Yousof ◽  
Ramesh Ranggasamy ◽  
Mohd Isa Wasiman ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Hplc Method ◽  
Chemical Markers ◽  
Step Method ◽  
Qualitative And Quantitative ◽  
Clinacanthus Nutans ◽  
Acetic Acid Water ◽  
Layer Chromatography ◽  
Tlc Separation

Clinacanthus nutans(family Acanthaceae) has been used for the treatment of inflammation and herpes viral infection. Currently, there has not been any report on the qualitative and quantitative determination of the chemical markers in the leaves ofC. nutans. TheC-glycosidic flavones such as shaftoside, isoorientin, orientin, isovitexin, and vitexin have been found to be major flavonoids in the leaves of this plant. Therefore, we had developed a two-step method using thin-layer chromatography (TLC) and high pressure liquid chromatography (HPLC) for the rapid identification and quantification of the flavonesC-glycosides inC. nutansleaves. The TLC separation of the chemical markers was achieved on silica gel 60 plate using ethyl acetate : formic acid : acetic acid : water (100 : 11 : 11 : 27 v/v/v/v) as the mobile phase. HPLC method was optimized and validated for the quantification of shaftoside, orientin, isovitexin, and vitexin and was shown to be linear in concentration range tested (0.4–200 μg/mL,r2≥ 0.996), precise (RSD ≤ 4.54%), and accurate (95–105%). The concentration of shaftoside, orientin, vitexin, and isovitexin inC. nutansleave samples was 2.55–17.43, 0.00–0.86, 0.00–2.01, and 0.00–0.91 mmol/g, respectively.

scholarly journals Rapid identification of Actinomycetaceae and related bacteria

1978 ◽  
Vol 8 (2) ◽  
pp. 127-133
Author(s):  
M Kilian
Keyword(s):  
Rapid Identification ◽  
Biochemical Properties ◽  
Cell Wall Composition ◽  
Gas Liquid Chromatography ◽  
Biochemical Tests ◽  
Identification Scheme ◽  
New Information ◽  
The Family ◽  
End Products

Identification of new isolates belonging to the family Actinomycetaceae requires extensive numbers of biochemical tests, supplemented with gas-liquid chromatography determination of fermentation end products and, often, analysis of cell wall composition. This paper describes the results of the testing of 162 strains of Actinomycetaceae and related taxa for 20 different enzymatic activities including phosphatases, esterases, aminopeptidases, and glycosidases. The results of all tests were read after 4 h of incubation. The results obtained in the study provide significant new information on the biochemical properties of these groups of bacteria. An identification scheme based upon 13 selected tests, which allow the identification of these groups of bacteria within 4 h, is proposed.

scholarly journals Combined Real-Time PCR and rpoB Gene Pyrosequencing for Rapid Identification of Mycobacterium tuberculosis and Determination of Rifampin Resistance Directly in Clinical Specimens

2010 ◽  
Vol 48 (4) ◽  
pp. 1182-1188 ◽  
Author(s):  
T. A. Halse ◽  
J. Edwards ◽  
P. L. Cunningham ◽  
W. J. Wolfgang ◽  
N. B. Dumas ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rpob Gene ◽  
Rapid Identification ◽  
Clinical Specimens ◽  
Rifampin Resistance
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