Effect of irradiation on the proteinase inhibitor activity and digestibility (in vitro) of safflower oilcake

1993 ◽  
Vol 70 (9) ◽  
pp. 935-937 ◽  
Author(s):  
Anu Joseph ◽  
Madhurima Dikshit
Keyword(s):  
Proteinase Inhibitor ◽  
Inhibitor Activity

A Simple and Rapid Method to Determine GPIIb/IIIa-Receptor Inhibitor Activity in Whole Blood

1999 ◽  
Vol 19 (03) ◽  
pp. 134-138
Author(s):  
Gitta Kühnel ◽  
A. C. Matzdorff
Keyword(s):  
Flow Cytometry ◽  
Platelet Count ◽  
Blood Sample ◽  
Platelet Activation ◽  
Whole Blood ◽  
Receptor Occupancy ◽  
Aggregate Formation ◽  
Inhibitor Activity ◽  
Receptor Inhibitor

SummaryWe studied the effect of GPIIb/IIIa-inhibitors on platelet activation with flow cytometry in vitro. Citrated whole blood was incubated with increasing concentrations of three different GPIIb/IIIa-inhibitors (c7E3, DMP728, XJ757), then thrombin or ADP were added and after 1 min the sample was fixed. Samples without c7E3 but with 0.1 U/ml thrombin had a decrease in platelet count. Samples with increasing concentrations of c7E3 had a lesser or no decrease in platelet count. The two other inhibitors (DMP 725, XJ757) gave similar results. GPIIb/IIIa-inhibitors prevent aggregate formation and more single platelets remain in the blood sample. The agonist-induced decrease in platelet count correlates closely with the concentration of the GPIIb/IIIa inhibitor and receptor occupancy. This correlation may be used as a simple measure for inhibitor activity in whole blood.

Proteinase-inhibitor activity and wound-inducible gene expression of the 22-kDa potato-tuber proteins

Planta ◽  
1991 ◽  
Vol 184 (4) ◽  
Author(s):  
Sang-Gon Suh ◽  
WillemJ. Stiekema ◽  
DavidJ. Hannapel
Keyword(s):  
Gene Expression ◽  
Potato Tuber ◽  
Proteinase Inhibitor ◽  
Inhibitor Activity ◽  
Inducible Gene Expression ◽  
Inducible Gene

Proteinase inhibitor activity in connective tissues

1974 ◽  
Vol 30 (6) ◽  
pp. 595-597 ◽  
Author(s):  
K. E. Kuettner ◽  
R. L. Croxen ◽  
R. Eisenstein ◽  
N. Sorgente
Keyword(s):  
Proteinase Inhibitor ◽  
Connective Tissues ◽  
Inhibitor Activity

scholarly journals In silico, in vitro and cellular analysis with a kinome-wide inhibitor panel correlates cellular LRRK2 dephosphorylation to inhibitor activity on LRRK2

2014 ◽  
Vol 7 ◽  
Author(s):  
Renée Vancraenenbroeck ◽  
Joren De Raeymaecker ◽  
Evy Lobbestael ◽  
Fangye Gao ◽  
Marc De Maeyer ◽  
...  
Keyword(s):  
In Silico ◽  
Inhibitor Activity ◽  
Cellular Analysis

Cigarette smoking, emphysema, and damage to alpha 1-proteinase inhibitor

1994 ◽  
Vol 266 (6) ◽  
pp. L593-L611 ◽  
Author(s):  
M. D. Evans ◽  
W. A. Pryor
Keyword(s):  
Cigarette Smoke ◽  
Proteinase Inhibitor ◽  
Inflammatory Cells ◽  
Lavage Fluid ◽  
Lung Lavage ◽  
Tissue Destruction ◽  
Phagocytic Cells ◽  
Lung Fluid

The proteinase-antiproteinase theory for the pathogenesis of emphysema proposes that the connective tissue destruction associated with emphysema arises from excessive proteinase activity in the lower respiratory tract. For this reason, the relative activities of neutrophil elastase and alpha 1-proteinase inhibitor (alpha 1-PI) are considered important. Most emphysema is observed in smokers; therefore, alpha 1-PI has been studied as a target for smoke-induced damage. Damage to alpha 1-PI in lung fluid could occur by several mechanisms involving species delivered to the lung by cigarette smoke and/or stimulated inflammatory cells. Oxidative damage to alpha 1-PI has received particular attention, since both cigarette smoke and inflammatory cells are rich sources of oxidants. In this article we review almost two decades of research on mechanistic studies of damage to alpha 1-PI by cigarette smoke and phagocytic cells in vitro, studies emphasizing the importance of elastinolytic activity in the pathogenesis of emphysema in vivo and studies of human lung lavage fluid to detect defects in alpha 1-PI at the molecular and functional levels.

scholarly journals Phycobilins as Potent Food Bioactive Broad-Spectrum Inhibitors Against Proteases of SARS-CoV-2 and Other Coronaviruses: A Preliminary Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Brahmaiah Pendyala ◽  
Ankit Patras ◽  
Chandravanu Dash
Keyword(s):  
Binding Affinity ◽  
Broad Spectrum ◽  
In Silico ◽  
Research Area ◽  
Therapeutic Drug ◽  
Inhibitor Activity ◽  
Current Vaccine ◽  
Mutant Strains ◽  
The Impact

In the 21st century, we have witnessed three coronavirus outbreaks: SARS in 2003, MERS in 2012, and the ongoing pandemic coronavirus disease 2019 (COVID-19). The search for efficient vaccines and development and repurposing of therapeutic drugs are the major approaches in the COVID-19 pandemic research area. There are concerns about the evolution of mutant strains (e.g., VUI – 202012/01, a mutant coronavirus in the United Kingdom), which can potentially reduce the impact of the current vaccine and therapeutic drug development trials. One promising approach to counter the mutant strains is the “development of effective broad-spectrum antiviral drugs” against coronaviruses. This study scientifically investigates potent food bioactive broad-spectrum antiviral compounds by targeting main protease (Mpro) and papain-like protease (PLpro) proteases of coronaviruses (CoVs) using in silico and in vitro approaches. The results reveal that phycocyanobilin (PCB) shows potential inhibitor activity against both proteases. PCB had the best binding affinity to Mpro and PLpro with IC50 values of 71 and 62 μm, respectively. Also, in silico studies with Mpro and PLpro enzymes of other human and animal CoVs indicate broad-spectrum inhibitor activity of the PCB. As with PCB, other phycobilins, such as phycourobilin (PUB), phycoerythrobilin (PEB), and phycoviolobilin (PVB) show similar binding affinity to SARS-CoV-2 Mpro and PLpro.

Association of Pulmonary Inflammation and Increased Microvascular Permeability During the Development of Bronchopulmonary Dysplasia: A Sequential Analysis of Inflammatory Mediators in Respiratory Fluids of High-Risk Preterm Neonates

PEDIATRICS ◽  
1994 ◽  
Vol 93 (5) ◽  
pp. 712-718
Author(s):  
Peter Groneck ◽  
Bettina Götze-Speer ◽  
Christian P. Speer ◽  
Martin Oppermann ◽  
Helmut Eiffert
Keyword(s):  
Birth Weight ◽  
Cell Count ◽  
Bronchopulmonary Dysplasia ◽  
Proteinase Inhibitor ◽  
Chemotactic Activity ◽  
Complement Component ◽  
Microvascular Permeability ◽  
Interleukin 8 ◽  
Preterm Neonates ◽  
Inhibitor Activity

Objectives. Bronchopulmonary dysplasia (BPD) of preterm neonates is associated with an increased recruitment of inflammatory cells into the airways. To evaluate further the role of inflammation in the pathogenesis of BPD, tracheobronchial aspirate fluid of neonates with birth weight <1200 g (n = 59) was sequentially analyzed in a prospective study. Methods. Tracheobronchial aspirate fluid was assessed for chemotactic activity, neutrophil cell count, concentrations of elastase-α1-proteinase inhibitor and activity of free elastase, concentrations of chemoattractants (complement component C5-derived anaphylatoxin, leukotrien B4, interleukin-8), and albumin concentrations as well as α1-proteinase inhibitor activity. The secretory component for immunoglobulin A was used as reference protein. Only specimens without evidence of microbiological colonization were studied. Results. In neonates with prolonged respiratory disease (BPD-risk neonates, n = 24, fraction of inspired oxygen ≥ 0.3 and/or peak inspiratory pressure ≥ 16 cm H2O at day 10 postnatal age, birth weight 892 ± 36 g, gestational age 27.2 ± 0.3 weeks) chemotactic activity, cell count, concentrations of the chemoattractants complement component C5-derived anaphylatoxin, leukotriene B4, interleukin-8, as well as levels of elastase-α1-proteinase inhibitor were significantly higher at day 10 and/or day 15 of postnatal age compared with neonates without chronic pulmonary disease (total n = 35; day 10, n = 11; day 15, n = 8). There was no difference in free elastolytic activity. Concentrations of albumin as well as α1-proteinase inhibitor activity were higher in BPD-risk patients on day 15, indicating an increased pulmonary leak. Conclusion. We conclude that preterm neonates at risk for the development of BPD show an enhanced inflammatory reaction in the lungs and an associated increase in pulmonary microvascular permeability. We speculate that inflammation may play an important role in the pathogenesis of BPD.

In Vitro Response of Human Chondrocytes to a Combination of Growth Factors and a Proteinase Inhibitor

Orthopedics ◽  
2012 ◽  
Vol 35 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Matthew A. Pifer ◽  
Leonard K. Kibuule ◽  
Tristan Maerz ◽  
Diane M. Studzinski ◽  
Kevin C. Baker ◽  
...  
Keyword(s):  
Growth Factors ◽  
Proteinase Inhibitor ◽  
Human Chondrocytes ◽  
In Vitro Response
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