Insulin sensitizes a cultured rat osteogenic sarcoma cell line to hormones which activate adenylate cyclase

1990 ◽  
Vol 46 (6) ◽  
pp. 401-405 ◽  
Author(s):  
Jill Hickman ◽  
Aidan McElduff
Keyword(s):  
Adenylate Cyclase ◽  
Cell Line ◽  
Osteogenic Sarcoma ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line

Modulation of glucose transport by parathyroid hormone and insulin in UMR 106–01, a clonal rat osteogenic sarcoma cell line

1995 ◽  
Vol 14 (2) ◽  
pp. 263-275 ◽  
Author(s):  
D M Thomas ◽  
S D Rogers ◽  
M W Sleeman ◽  
G M Pasquini ◽  
F R Bringhurst ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cell Line ◽  
Glucose Transport ◽  
Transport System ◽  
Osteogenic Sarcoma ◽  
Regulatory Subunit ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Glucose Transport System ◽  
Umr 106

ABSTRACT This study characterizes the actions of insulin and parathyroid hormone (PTH) on the glucose transport system in the rat osteogenic sarcoma cell line UMR 106–01, which expresses a number of features of the osteoblast phenotype. Using [1,2-3H]2-deoxyglucose (2-DOG) as a label, UMR 106–01 cells were shown to possess a glucose transport system which was enhanced by insulin. In contrast, PTH influenced glucose transport in a biphasic manner with a stimulatory effect at 1 h and a more potent inhibitory effect at 16 h on basal and insulin-stimulated 2-DOG transport. To explore the mechanism of PTH action, a direct agonist of cAMP-dependent protein kinase (PKA) was tested. 8-Bromo-cAMP had no acute stimulatory effect but inhibited basal and insulin-stimulated 2-DOG transport at 16 h. This result suggested that the prolonged, but not the acute, effect of PTH was mediated by the generation of cAMP. Further studies with the cell line UMR 4–7, a UMR 106–01 clone stably transfected with an inducible mutant inactive regulatory subunit of PKA, confirmed that the inhibitory but not the stimulatory effect of PTH was mediated by the PKA pathway. Northern blot data indicated that the prolonged inhibitory effects of PTH and 8-bromo-cAMP on glucose transport were likely to be mediated in part by reduction in the levels of GLUT1 (HepG2/brain glucose transporter) mRNA.

Expression of transferrin and vitamin D-binding protein genes in an osteogenic sarcoma cell line

1990 ◽  
Vol 186 (2) ◽  
pp. 385-389 ◽  
Author(s):  
Gwendolyn S. Adrian ◽  
Funmei Yang ◽  
Dana T. Graves ◽  
James M. Buchanan ◽  
Barbara H. Bowman
Keyword(s):  
Vitamin D ◽  
Cell Line ◽  
Binding Protein ◽  
Osteogenic Sarcoma ◽  
Vitamin D Binding Protein ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line

CAFFEINE INDUCES APOPTOSIS IN THE OSTEOGENIC SARCOMA CELL LINE.

2007 ◽  
Vol 55 (1) ◽  
pp. S252
Author(s):  
T. Shimizu ◽  
T. Nakamoto ◽  
Y. Suzuki ◽  
M. Yazdani ◽  
S. Gottschalk ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteogenic Sarcoma ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line

scholarly journals Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line

1981 ◽  
Vol 43 (5) ◽  
pp. 582-587 ◽  
Author(s):  
M J Embleton ◽  
B Gunn ◽  
V S Byers ◽  
R W Baldwin
Keyword(s):  
Monoclonal Antibody ◽  
Cell Line ◽  
Osteogenic Sarcoma ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line

Production of a strain of human osteogenic sarcoma cell line HOS transplantable into nude mice and rats

1988 ◽  
Vol 106 (4) ◽  
pp. 1470-1471 ◽  
Author(s):  
E. S. Revazova ◽  
Yu. N. Solov'ev ◽  
T. M. Khizhnyakova
Keyword(s):  
Cell Line ◽  
Nude Mice ◽  
Osteogenic Sarcoma ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Nude Mice And Rats
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