Effect of scavengers of active oxygen species and pretreatment with acetyl-salicylic acid on the injury to cultured endothelial cells by thrombin-stimulated platelets

1989 ◽  
Vol 25 (3) ◽  
pp. 276-282 ◽  
Author(s):  
Tor Larsen ◽  
Mona Berger Sørensen ◽  
Randi Olsen ◽  
Leif Jørgensen
Keyword(s):  
Endothelial Cells ◽  
Salicylic Acid ◽  
Active Oxygen Species ◽  
Active Oxygen ◽  
Acetyl Salicylic Acid ◽  
Oxygen Species ◽  
Cultured Endothelial Cells

scholarly journals Protective activity of propofol, Diprivan®and intralipid against active oxygen species

1998 ◽  
Vol 7 (5) ◽  
pp. 327-333 ◽  
Author(s):  
M. Mathy-Hartert ◽  
G. Deby-Dupont ◽  
P. Hans ◽  
C. Deby ◽  
M. Lamy
Keyword(s):  
Endothelial Cells ◽  
Active Oxygen Species ◽  
Oxidant Stress ◽  
Antioxidant Properties ◽  
Active Oxygen ◽  
Oxygen Species ◽  
Protective Effects ◽  
Extracellular Medium ◽  
Lower Effect ◽  
Dose Dependent

We separately studied the antioxidant properties of propofol (PPF), Diprivan®(the commercial form of PPF) and intralipid (IL) (the vehicle solution of PPF in Diprivan®) on active oxygen species produced by phorbol myristate acetate (10-6M)-stimulated human polymorphonuclear leukocytes (PMN: 5 × 105cells/ assay), human endothelial cells (5 × 105cells/assay) or cell-free systems (NaOCl or H2O2/peroxidase systems), using luminol (10-4M)-enhanced chemiluminescence (CL). We also studied the protective effects of Diprivan®on endothelial cells submitted to an oxidant stress induced by H2O2/MPO system: cytotoxicity was assessed by the release of preincorporated51Cr. Propofol inhibited the CL produced by stimulated PMN in a dose dependent m anner (until 5 × 10-5M, a clinically relevant concentration), while Diprivan®and IL were not dose-dependent inhibitors. The CL produced by endothelial cells was dosedependently inhibited by Diprivan®and PPF, and weakly by IL (not dose-dependent). In cell free systems, dose-dependent inhibitions were obtained for the three products with a lower effect for IL. Diprivan®efficaciously protected endothelial cells submitted to an oxidant stress, while IL was ineffective. By HPLC, we demonstrated that PPF was not incorporated into the cells. The drug thus acted by scavenging the active oxygen species released in the extracellular medium. IL acted in the same manner, but was a less powerful antioxidant.

scholarly journals Some biochemical reactions of strawberry plants to infection with Botrytis cinerea and salicylic acid treatment

2013 ◽  
Vol 47 (2) ◽  
pp. 73-81 ◽  
Author(s):  
Urszula Małolepsza ◽  
Henryk Urbanek ◽  
Justyna Polit
Keyword(s):  
Superoxide Dismutase ◽  
Salicylic Acid ◽  
Phenolic Compounds ◽  
Botrytis Cinerea ◽  
Acid Treatment ◽  
Active Oxygen Species ◽  
Active Oxygen ◽  
Oxygen Species ◽  
Salicylic Acid Treatment ◽  
Biochemical Reactions

The reactions of strawberry plants to infection with <i>B. cinerea</i> and treatment with salicylic acid has been studied. Infection of leaves with <i>B. cinerea</i> resulted in early increases in active oxygen species generation, superoxide dismutase and peroxidase activities and phenolic compounds content. Some increases of the above reactions were noticed in plants treated with salicylic acid but not in the plants treated with SA and then later infected with <i>B. cinerea</i>.

Characterization and functional properties of new everbearing strawberry (Fragaria x ananasa Duch.) cultivar, ‘Summertiara’ berries

2014 ◽  
Vol 4 (1) ◽  
pp. 1 ◽  
Author(s):  
Takeshi Nagai ◽  
Misuzu Tamai ◽  
Masato Sato ◽  
Yasuhiro Tanoue ◽  
Norihisa Kai ◽  
...  
Keyword(s):  
Vitamin C ◽  
Sensory Evaluation ◽  
Functional Properties ◽  
Active Oxygen Species ◽  
Active Oxygen ◽  
Antihypertensive Activity ◽  
Oxygen Species ◽  
Total Anthocyanins ◽  
Strawberry Cultivar ◽  
The Stability

Background: In recent years, a new everbearing strawberry cultivar, ‘Summertiara’ was cultivated to supply the strawberries in pre-harvest season from July to October in Japan. For highly research and development of processing of this cultivar, ‘Summertiara’ berries, the objective of this study was to characterize these berries, with relation to chemical parameters, total phenols, total flavonoids, total vitamin C, and total anthocyanins, and was to investigate the solubility and the stability of anthocyanins from the berries. Moreover, the functional properties such as antioxidative activity, active oxygen species scavenging activity, and antihypertensive activity were also evaluated.Methods: Chemical analysis, colour measurement, and sensory evaluation of new everbearing strawberry cultivar, ‘Summertiara’ berries were performed. Next, the solubility of anthocyanins from the berries and stability of these against pH, temperature, and an incandescent lighting were investigated. Moreover, functional properties of the extracts prepared from berries were elucidated using 5 different methods.Results: The contents of water, proteins, lipids, carbohydrates, and ash were the same as those of other cultivar berries. The sugar-acid ratio in the berries was low; these were acidulous. By sensory evaluation, the main factors were vivid red colour, aroma, and acidity. The berries were rich in phenols, flavonoids, vitamin C, and anthocyanins. The anthocyanins of the berries became unstable by heat treatment and light exposures such as visible rays. On the other hand, the extracts prepared from the berries showed the functionalities such as antioxidant activity, active oxygen species scavenging activities, and antihypertensive activity.  Conclusions: The strawberry cultivar, ‘Summertiara’ berries were the most suitable for processing ingredient of strawberry-derived products with superior health promoting functionalities.Keywords: Summertiara, everbearing strawberry cultivar, characterization, sensory evaluation, color and storage, functional property

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