Cyclic fatty acid monomer: Isolation and purification with solid phase extraction

1989 ◽  
Vol 66 (11) ◽  
pp. 1593-1595 ◽  
Author(s):  
Jose A. Rojo ◽  
Edward G. Perkins

2009 ◽  
Vol 100 (24) ◽  
pp. 6385-6390 ◽  
Author(s):  
Min Li ◽  
Patrisha J. Pham ◽  
Tao Wang ◽  
Charles U. Pittman ◽  
Tingyu Li




2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Alexander M. Wathne ◽  
Hanne Devle ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg

Fatty acid (FA) profiles of the species Tettigonia viridissima, Chorthippus biguttulus, and Chorthippus brunneus were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in T. viridissima, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from T. viridissima were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For C. brunneus and C. biguttulus, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, n-3 FAs, and n-6 FAs of each species, and the n-6/n-3 ratio, were subsequently discussed.



2017 ◽  
Vol 80 (11) ◽  
pp. 1651-1658 ◽  
Author(s):  
Hongliu Liu ◽  
Chengcheng Gong ◽  
Zhixin Liao ◽  
Tao Chen ◽  
Yulin Li




Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1085
Author(s):  
Marjeta Mencin ◽  
Maja Mikulic-Petkovsek ◽  
Robert Veberič ◽  
Petra Terpinc

A solid-phase extraction (SPE) technique was developed and optimised for isolation and concentration of extractable and bound phenolic acids from germinated spelt seeds, for analysis by liquid chromatography–mass spectrometry. Samples initially underwent solvent extraction under different conditions to maximise the yield of phenolic antioxidants. Optimal extraction conditions for extractable phenolics were absolute methanol as solvent, sample-to-methanol ratio 1:9, and reconstitution in non-acidified water. The bound phenolics were extracted from sample pellets using hydrolysis with 2 M NaOH, acidification of the hydrolysate with formic acid, and simultaneous isolation and purification using Strata X polymeric RP tubes. Compared to liquid–liquid extraction, this direct SPE protocol has significant advantages in terms of higher extraction efficiencies of total and individual phenolics and their antioxidant activities. These data suggest that direct SPE represents a rapid and reliable method for quantitative analysis of both the extractable and the commonly overlooked bound phenolics in Triticum spelta seeds.



2015 ◽  
Vol 64 (12) ◽  
pp. 1251-1258 ◽  
Author(s):  
Ryo Sasaki ◽  
Masatoshi Umezawa ◽  
Satoru Tsukahara ◽  
Takashi Ishiguro ◽  
Shinichi Sato ◽  
...  


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