Solid phase extraction and enrichment of essential fatty acid methyl esters from soy-derived biodiesel by novel π-complexing sorbents

2009 ◽  
Vol 100 (24) ◽  
pp. 6385-6390 ◽  
Author(s):  
Min Li ◽  
Patrisha J. Pham ◽  
Tao Wang ◽  
Charles U. Pittman ◽  
Tingyu Li
Keyword(s):  
Fatty Acid ◽  
Solid Phase Extraction ◽  
Essential Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Solid Phase ◽  
Methyl Esters ◽  
Phase Extraction ◽  
Acid Methyl

scholarly journals Home-made silver ion solid-phase extraction system for the analysis of trans fatty acids: comparison with commercial Discovery Ag-Ion SPE

Chemija ◽  
2018 ◽  
Vol 29 (1) ◽  
Author(s):  
Vilius Poškus ◽  
Vida Vičkačkaitė ◽  
Julita Dargytė ◽  
Gintautas Brimas
Keyword(s):  
Fatty Acids ◽  
Solid Phase Extraction ◽  
Trans Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Solid Phase ◽  
Methyl Esters ◽  
Human Adipose Tissue ◽  
Silver Ion ◽  
Phase Extraction ◽  
Acid Methyl

A home-made silica-based silver ion solid-phase extraction (Ag+-SPE) system for the fractionation and subsequent gas chromatographic analysis of trans fatty acids in human adipose tissue is developed and examined. Analytical characteristics of the home-made Ag+-SPE column were compared with those of the commercial Discovery Ag-Ion SPE column and it was demonstrated that the both columns can be applied for the fractionation of fatty acid methyl esters.

scholarly journals Identification and Quantification of Fatty Acids in T. viridissima, C. biguttulus, and C. brunneus by GC-MS

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Alexander M. Wathne ◽  
Hanne Devle ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Solid Phase Extraction ◽  
Free Fatty Acids ◽  
Dry Matter ◽  
Solid Phase ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Phase Extraction ◽  
Chorthippus Biguttulus

Fatty acid (FA) profiles of the species Tettigonia viridissima, Chorthippus biguttulus, and Chorthippus brunneus were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in T. viridissima, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from T. viridissima were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For C. brunneus and C. biguttulus, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, n-3 FAs, and n-6 FAs of each species, and the n-6/n-3 ratio, were subsequently discussed.

Simultaneous Analysis of Oil-Soluble, Basic, and Acidic Illegal Dyes in Foods Using Liquid Chromatography–Diode-Array Detection

2017 ◽  
Vol 100 (4) ◽  
pp. 1102-1109 ◽  
Author(s):  
Yoko Uematsu ◽  
Toshiko Mizumachi ◽  
Kimio Monma
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Solid Phase ◽  
Soft Drink ◽  
Methyl Esters ◽  
Water Soluble ◽  
Diode Array ◽  
Diode Array Detection ◽  
Acid Methyl ◽  
Array Detection

Abstract A method for simutaneously detecting 8 oil-soluble and 10 water-soluble (3 basic and 7 acidic) illegal dyes in foods was developed. The sample was mixed with water, followed by methanol and tetrahydrofuran. Transesterification with sodium methoxide was applied to the mixture, which allowed the triglycerides in the sample to be converted to fatty acid methyl esters. This treatment resulted in a biphasic mixture. Oil-soluble dyes and fatty acid methyl esters were deposited in the upper organic phase, which was cleaned using a silica-gel solid-phase extraction (SPE) column to remove the fatty acid methyl esters from the solution. The water-soluble dyes were deposited in the aqueous phase, and an Oasis hydrophilic–lipophilic-balanced SPE column was used to remove polar matrix components from the solution. The resulting dyes were subsequently analyzed via LC–diode-array detection using a single method. The practical LODs of the samples were defined as the lowest spiked dye concentrations at which the similarity coefficient for the spectra of the LC test solution and the corresponding reference standard solution were greater than 0.99, thus affording LODs of 0.5–1.0 µg/g. Recoveries of the dyes at a spiking level of 5.0 µg/g from soft drink, chili sauce, and mustard were generally greater than 70%. Recoveries from paprika powder were between 33 and 103%.

scholarly journals A method for separation of phosphatidylcholine, triacylglycerol, non-esterified fatty acids and cholesterol esters from plasma by solid-phase extraction

2000 ◽  
Vol 84 (5) ◽  
pp. 781-787 ◽  
Author(s):  
Graham C. Burdge ◽  
Paul Wright ◽  
Amanda E. Jones ◽  
Stephen A. Wootton
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Lipid Classes ◽  
Phase Extraction ◽  
Rapid Separation ◽  
Esterified Fatty Acids

Efficient isolation of individual lipid classes is a critical step in the analysis of plasma and lipoprotein fatty acid compositions. Whilst good separations of total lipid extracts are possible by TLC, this method is time consuming and a major rate-limiting step when processing large numbers of specimens. A method for rapid separation of phosphatidylcholine (PC), non-esterified fatty acids (NEFA), cholesterol ester (CE) and triacylglycerol (TAG) from total plasma lipid extracts by solid-phase extraction (SPE) using aminopropyl silica columns has been developed and validated. Following initial separation of polar and neutral lipids, individual classes were isolated by application of solvents with increasing polarity. Recoveries for combined plasma extraction with chloroform–methanol and SPE were (%): PC 74·2 (SD 7·5), NEFA 73·6 (sd 8·3), CE 84·9 (sd 4·9), and TAG 86·8 (sd 4·9), which were significantly greater for TAG and NEFA than by TLC (P<0·001). Both GC–flame ionisation detector and GC-MS analysis of fatty acid methyl esters demonstrated that there was no cross-contamination between lipid classes. Measurements of repeatability of fatty acid composition for TAG, PC, CE and NEFA fractions showed similar CV for each fatty acid. The magnitude of the CV appeared to be related inversely to the fractional fatty acid concentration, and was greatest at concentrations of less than 1 g/100 g total fatty acids. There was no evidence of selective elution of individual fatty acid or CE species. In conclusion, this method represents an efficient, rapid alternative to TLC for isolation of these lipid classes from plasma.

Sign in / Sign up

Export Citation Format

Share Document