Early expression of vimenti in human mammary cultures

In Vitro ◽  
1985 ◽  
Vol 21 (6) ◽  
pp. 321-327 ◽  
Author(s):  
Shahnaz Hashmi Dairkee ◽  
Carlene M. Blayney ◽  
David M. Asarnow ◽  
Helene S. Smith ◽  
Adeline J. Hackett
Keyword(s):  
Early Expression

Early expression of manual lateralization in bipedal marsupials.

2017 ◽  
Vol 131 (3) ◽  
pp. 225-230 ◽  
Author(s):  
Andrey Giljov ◽  
Karina Karenina ◽  
Janeane Ingram ◽  
Yegor Malashichev
Keyword(s):  
Early Expression

Presbyterian Preaching

2019 ◽  
pp. 506-523
Author(s):  
Thomas G. Long
Keyword(s):  
Digital Media ◽  
Emotional Experience ◽  
New School ◽  
Intellectual Content ◽  
The Bible ◽  
The Reformation ◽  
Korean Presbyterian ◽  
Early Expression ◽  
History Of ◽  
School Conflict

Presbyterian preaching grew from roots in the Reformation, particularly the Calvinist wing. The fullest early expression of the character of Presbyterian preaching is in the Westminster Standards, documents produced in England by an assembly of Calvinist clergy and laymen in the mid-seventeenth century. These documents described the key qualities of Reformed, and thus Presbyterian, preaching: sermons grounded in the Bible, containing significant doctrinal content, and aimed at teaching and edifying congregants.The authors of the Westminster Standards prescribed preaching that was substantive and lively, filled with biblical and doctrinal content, and touched the hearts of hearers. Throughout the history of Presbyterian preaching, however, these twin goals were often difficult to attain. This tension between intellectual, content-centered preaching and more emotional, experience-centered preaching among Presbyterian is evident in such events as the Old Side–New Side controversy in the mid-1700s and the Old School–New School conflict from 1837 to 1869 (both in America), in Scottish Presbyterian preaching in the early nineteenth century, and in Korean Presbyterian preaching during the end of the twentieth and the beginning of the twentieth century.Today as many Presbyterian preachers use digital media and conversational-style sermons, a strong desire continues for preaching that is clear, deeply theological and biblical, impassioned, and relevant.

Online Kernel-Based Structured Output SVM for Early Expression Detection

2019 ◽  
Vol 26 (9) ◽  
pp. 1305-1309 ◽  
Author(s):  
Liping Xie ◽  
Junsheng Zhao ◽  
Haikun Wei ◽  
Kanjian Zhang ◽  
Guochen Pang
Keyword(s):  
Early Expression ◽  
Structured Output

scholarly journals Transcriptional Pausing Caused by NELF Plays a Dual Role in Regulating Immediate-Early Expression of the junB Gene

2006 ◽  
Vol 26 (16) ◽  
pp. 6094-6104 ◽  
Author(s):  
Masatoshi Aida ◽  
Yexi Chen ◽  
Koichi Nakajima ◽  
Yuki Yamaguchi ◽  
Tadashi Wada ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Transcription Initiation ◽  
Mrna Level ◽  
Proximal Region ◽  
Negative Regulation ◽  
Immediate Early ◽  
Dual Function ◽  
Early Expression ◽  
Transcriptional Pausing ◽  
Promoter Proximal Region

ABSTRACT Human 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II (RNAPII) in vitro. However, the physiological roles of this negative regulation are not well understood. Here, by using a number of approaches to identify protein-DNA interactions in vivo, we show that DSIF- and NELF-mediated transcriptional pausing has a dual function in regulating immediate-early expression of the human junB gene. Before induction by interleukin-6, RNAPII, DSIF, and NELF accumulate in the promoter-proximal region of junB, mainly at around position +50 from the transcription initiation site. After induction, the association of these proteins with the promoter-proximal region continues whereas RNAPII and DSIF are also found in the downstream regions. Depletion of a subunit of NELF by RNA interference enhances the junB mRNA level both before and after induction, indicating that DSIF- and NELF-mediated pausing contributes to the negative regulation of junB expression, not only by inducing RNAPII pausing before induction but also by attenuating transcription after induction. These regulatory mechanisms appear to be conserved in other immediate-early genes as well.

Expression of the SMP antigen by oligodendrocytes in the developing avian central nervous system

Development ◽  
1989 ◽  
Vol 107 (4) ◽  
pp. 825-833
Author(s):  
P. Cameron-Curry ◽  
C. Dulac ◽  
N.M. Le Douarin
Keyword(s):  
Monoclonal Antibody ◽  
Schwann Cell ◽  
Basic Protein ◽  
Culture Conditions ◽  
Cell Marker ◽  
Cellular Expression ◽  
Early Expression ◽  
Different Parts

Expression of the avian antigen SMP (Schwann cell Myelin Protein, Mr 75-80000), first characterized in the PNS with a monoclonal antibody as an early and strictly specific Schwann cell marker, was further studied in the CNS. Comparing SMP immunoreactive areas in the different parts of the CNS with those expressing the Myelin Basic Protein (MBP), we showed a strict colocalisation of both phenotypes. In vitro, MBP+ oligodendrocytes express the surface antigen SMP as well. SMP cellular expression was followed in situ and in culture using nervous tissues from embryos at different stages. We were thus able to detect an early expression of this marker by oligodendroblasts before the first appearance of MBP immunoreactivity. We have also identified a subpopulation of SMP+/MBP- and SMP+/GC- cells, which persists under our culture conditions as precursors remaining in an immature state.

Analysis of Hox-4.5 and Hox-3.6 expression during newt limb regeneration: differential regulation of paralogous Hox genes suggest different roles for members of different Hox clusters

Development ◽  
1993 ◽  
Vol 117 (4) ◽  
pp. 1397-1407 ◽  
Author(s):  
H.G. Simon ◽  
C.J. Tabin
Keyword(s):  
Hox Genes ◽  
Limb Regeneration ◽  
Differential Regulation ◽  
Epithelial Tissue ◽  
Cdna Clones ◽  
Rnase Protection ◽  
Adult Kidney ◽  
Early Expression ◽  
Hox Clusters ◽  
Newt Limb

Adult urodele amphibians can regenerate their limbs and tail. Based on their roles in other developing systems, Hox genes are strong candidates for genes that play a role in regulating pattern formation during regeneration. There are four homologous clusters of Hox genes in vertebrate genomes. We isolated cDNA clones of two newt homeobox genes from homologous positions within two Hox clusters; Hox-4.5 and Hox-3.6. We used RNase protection on nonamputated (normal) and regenerating newt appendages and tissue to compare their transcriptional patterns. Both genes show increased expression upon amputation with similar kinetics. Hox-4.5 and Hox-3.6 transcription is limited to the mesenchymal cells in the regenerates and is not found in the epithelial tissue. In addition to regenerating appendages, both genes are transcriptionally active in adult kidney of the newt. Striking differences were found in the regulation of Hox-4.5 and Hox-3.6 when they were compared in unamputated limbs and in regenerating forelimbs versus regenerating hindlimbs. Hox-4.5 is expressed in the blastema of regenerating fore- and hindlimbs, but Hox-4.5 transcripts are not detectable in normal limbs. In contrast, Hox-3.6 transcripts are found exclusively in posterior appendages, but are present in normal as well as regenerating hindlimbs and tails. Hox-4.5 is also expressed at a higher level in proximal (mid-humerus) regenerates than in distal ones (mid-radius). When we proximalized the positional memory of a distal blastema with retinoic acid, we find that the early expression level of Hox-4.5 is also proximalized. When the expression of these genes is compared to the expression of two previously reported newt Hox genes, a consistent pattern emerges, which can be interpreted in terms of differential roles for the different Hox clusters in determining regenerative limb morphology.

Conserved segmental expression of Krox-20 in the vertebrate hindbrain and its relationship to lineage restriction

Development ◽  
1991 ◽  
Vol 113 (Supplement_2) ◽  
pp. 59-62 ◽  
Author(s):  
M. Angela Nieto ◽  
Leila C. Bradley ◽  
David G. Wilkinson
Keyword(s):  
Expression Pattern ◽  
Zinc Finger ◽  
Cell Lineage ◽  
Zinc Finger Gene ◽  
Early Stages ◽  
Cellular Events ◽  
Early Expression ◽  
Segmental Expression

The zinc-finger gene Krox-20 is expressed in two alternating segments, rhombomeres (r) 3 and 5, in the developing mouse hindbrain. This expression pattern is established prior to rhombomere formation in the mouse, but it is not known how the timing of expression relates to cellular events of segmentation, such as lineage restriction. We have cloned Krox-20 sequences from Xenopus and the chick and shown that its alternating expression pattern is conserved in these systems, suggesting that its role in hindbrain development is conserved. Analysis of the early stages of Krox-20 expression in the chick show that both domains of expression precede the restriction of cell lineage to specific rhombomeres, consistent with a role of this gene in early events of hindbrain segmentation. The finding that expression is not coincident with lineage restriction indicates that early expression may not reflect an irreversible commitment of cells to r3 and r5 and/or may be mosaic.

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