Growth-phase associated changes in the reproductive capacity, intracellular polyamines, and antilysozyme activity inEscherichia coli batch culture

Microbiology ◽  
2000 ◽  
Vol 69 (1) ◽  
pp. 113-115 ◽  
Author(s):  
O. V. Bukharin ◽  
V. A. Gritsenko ◽  
A. G. Tkachenko ◽  
O. Ya. Salakhetdinova

FEBS Letters ◽  
1998 ◽  
Vol 421 (3) ◽  
pp. 237-242 ◽  
Author(s):  
Anne-Marie K Wenthzel ◽  
Martin Stancek ◽  
Leif A Isaksson




1999 ◽  
Vol 181 (14) ◽  
pp. 4435-4436 ◽  
Author(s):  
Georgina Macintyre ◽  
Photini Pitsikas ◽  
Claire G. Cupples

ABSTRACT Using rabbit polyclonal antibodies, we have shown that the Dcm cytosine methylase of Escherichia coli is maintained at a constant level during cell growth, while Vsr endonuclease levels are growth phase dependent. Decreased production of Vsr relative to Dcm during the log phase may contribute substantially to the mutability of 5-methylcytosine.



1985 ◽  
Vol 27 (6) ◽  
pp. 887-889 ◽  
Author(s):  
R. H. Cumming ◽  
J. Tuffnell ◽  
G. Street






Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.



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