Studies on the serum lipid fractions in the liver diseases, diabetes mellitus and obesity analyzed with thin layer chromatography

1966 ◽  
Vol 1 (1) ◽  
pp. 37-38
Author(s):  
M. Kishikawa ◽  
H. Marino ◽  
T. Mokuno ◽  
K. Nagahara ◽  
H. Ishikawa ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Liver Diseases ◽  
Serum Lipid ◽  
Lipid Fractions ◽  
Layer Chromatography

Lipids in Sphagnum mosses of various ages

1979 ◽  
Vol 57 (12) ◽  
pp. 1335-1339 ◽  
Author(s):  
Pirjo Karunen ◽  
Heikki Mikola ◽  
Reino Linko ◽  
Erkki K. Euranto
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Lipid Content ◽  
Dry Weight ◽  
Polar Lipids ◽  
Wax Esters ◽  
Sphagnum Mosses ◽  
Lipid Fractions ◽  
Sphagnum Fuscum ◽  
Layer Chromatography

The lipid content in the annual increments of different ages (1 to 5 years) of Sphagnum fuscum (Schimp.) Klinggr., S. angustifolium (Russow) C. Jens., and S. papillosum Lindb. has been determined by thin-layer chromatography. It was highest in the youngest portion of the moss shoot, ranging from 4.8 to 5.2% of the dry weight and decreased with increasing age to about 40–60% of the original value.The main lipid fractions of the youngest section, the capitulum, of the S. fuscum shoot were steryl and wax esters, triglycerides, and more polar lipids and accounted for 0.4, 0.3, and 3.5%, respectively, of the dry weight. The amounts of these lipids were found to decrease during senescence at independent rates.

scholarly journals Antidiabetic Compounds in Syzygium cumini Decoction and Ready to Serve Herbal Drink

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
P. R. D. Perera ◽  
S. Ekanayake ◽  
K. K. D. S. Ranaweera
Keyword(s):  
Diabetes Mellitus ◽  
Thin Layer ◽  
Sequential Extraction ◽  
Gallic Acid ◽  
Thin Layer Chromatography ◽  
Ellagic Acid ◽  
Syzygium Cumini ◽  
Health Promoting ◽  
Layer Chromatography ◽  
Specific Health

Herbal beverages with desirable sensory attributes are an ideal way to offer consumers with phytochemicals having specific health promoting functionalities. Syzygium cumini bark decoction is used in treating diabetes mellitus in Ayurveda medicine. This work attempted to prove the presence of antidiabetic compounds in the S. cumini decoction and the ready to serve (RTS) herbal drink developed using the decoction. Activity guided fractionation of the decoction of the S. cumini was carried out by sequential extraction with organic solvents of different polarities. Thin Layer Chromatography (TLC) with authentic compounds and HPLC were performed for identification and confirmation of the compounds in the decoction and the RTS herbal drink. Presence of gallic and ellagic acids in the decoction and RTS herbal drink was proven and confirmed with HPLC. The LC UV-VIS spectra of the two compounds were identical with the corresponding spectra of the library. Further, gallic acid and umbelliferone were determined as the active compounds in the decoction by TLC and were confirmed by cochromatography with authentic compounds. The present investigation confirmed the presence of gallic acid, ellagic acid, and umbelliferone which are proven to have antidiabetic activities in the decoction and the RTS herbal drink prepared with the decoction.

Composition and Distribution of Lipids in Tissues of Bogue (Boops boops)

1985 ◽  
Vol 40 (7-8) ◽  
pp. 562-565 ◽  
Author(s):  
Vassihos M. Kapoulas ◽  
Sofia Miniadis-Meimaroglou
Keyword(s):  
Thin Layer ◽  
Neutral Lipid ◽  
Thin Layer Chromatography ◽  
Glyceryl Ether ◽  
Fatty Alcohols ◽  
Total Lipids ◽  
Triglyceride Fraction ◽  
Lipid Fractions ◽  
Layer Chromatography ◽  
Boops Boops

Abstract Total lipids from liver, head, skin and muscle of Bogue were separately isolated and their composition was investigated by a combination of analytical determinations, and column and thin layer chromatography. The major components of the neutral lipid fractions from all tissues studied were triglycerides, followed by cholesterol. The triglyceride fraction of skin and head contains significant amounts of glyceryl ether analogs. Low contents of free fatty alcohols were also identified, decreasing in the order: head, muscle, skin and liver. The major components of all phospholipid fractions was phosphatidylcholine (viz. 36-59% of total phospholipids) followed by phosphatidylethanolamine (viz. 23-34% of total phos­ pholipids). Low amounts of sphingomyelin and phosphatidylserine were also identified in all cases. All the tissues studied were found to contain plasmalogens, as well as glyceryl ether analogs in both, the depot fats and the phospholipid fractions.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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